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    Recombinant polymerases for improved single molecule sequencing
    1.
    发明授权
    Recombinant polymerases for improved single molecule sequencing 有权
    用于改进单分子测序的重组聚合酶

    公开(公告)号：US08999676B2

    公开(公告)日：2015-04-07

    申请号：US13176697

    申请日：2011-07-05

    申请人： Robin Emig ,  Lei Jia ,  Satwik Kamtekar ,  Erik Miller ,  Colleen Cutcliffe ,  Walter Lee

    发明人： Robin Emig ,  Lei Jia ,  Satwik Kamtekar ,  Erik Miller ,  Colleen Cutcliffe ,  Walter Lee

    IPC分类号： C12N15/54 ,  C12N9/00 ,  C12N9/12 ,  C12Q1/68

    CPC分类号： C12N9/1252 ,  C12N9/1241 ,  C12P19/34 ,  C12Q1/6869

    摘要： Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

    摘要翻译： 提供了包含重组DNA聚合酶的组合物，其包括赋予聚合酶修饰性质以增强单分子测序的氨基酸取代，插入，缺失和/或外源特征。 这种性质可以包括增强的金属离子配位，降低的核酸外切酶活性，在聚合酶动力学循环的一个或多个步骤中降低的反应速率，降低的支化部分，改变的辅因子选择性，增加的产率，增加的热稳定性，增加的准确度，增加的速度，增加的读长度， 等等。 还提供了编码具有上述表型的聚合酶的核酸，以及使用这种聚合酶制备DNA或顺序DNA模板的方法。

    DISPENSER CONTROL SYSTEMS AND METHODS
    3.
    发明申请
    DISPENSER CONTROL SYSTEMS AND METHODS 审中-公开
    分配器控制系统和方法

    公开(公告)号：US20090317311A1

    公开(公告)日：2009-12-24

    申请号：US12524052

    申请日：2008-01-31

    申请人： Andrew J. Cocking ,  Michael A. Steed ,  Erik Miller

    发明人： Andrew J. Cocking ,  Michael A. Steed ,  Erik Miller

    IPC分类号： B01D11/02

    CPC分类号： D06F39/026 ,  A47L15/0049 ,  A47L15/0055 ,  A47L15/4436 ,  A47L15/4463 ,  A47L2401/30 ,  A47L2501/07 ,  A47L2501/26 ,  D06F33/02 ,  D06F2202/12 ,  D06F2204/02

    摘要： A method of operating a dispensing system having a material delivery cycle. In some embodiments, the material delivery cycle includes supplying water to a receptacle, performing an operation intended to release a material into the water, and delivering the material to a downstream component. The first step of the method is to initiate the material delivery cycle. Next, a conductivity proximate to the receptacle is monitored. Additionally, one or more error conditions are identified during the material delivery cycle based at least partially on the monitored conductivity.

    摘要翻译： 一种操作具有材料输送循环的分配系统的方法。 在一些实施例中，材料递送循环包括向容器供水，执行旨在将材料释放到水中的操作，以及将材料输送到下游部件。 该方法的第一步是启动物料输送周期。 接下来，监测靠近容器的电导率。 另外，至少部分地基于所监测的电导率，在材料递送周期期间识别一个或多个错误条件。