-
公开(公告)号：US10167497B2
公开(公告)日：2019-01-01
申请号：US15214494
申请日：2016-07-20
IPC分类号： C12Q1/32
摘要： The present invention provides for stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives of formula (I), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits.
-
公开(公告)号：US09926536B2
公开(公告)日：2018-03-27
申请号：US14810070
申请日：2015-07-27
发明人： Marco Bocola , Hartmut Duefel , Erik Uwe Arango Gutierrez , Dieter Heindl , Thomas Meier , Hemanshu Mundhada , Ulrich Schwaneberg , Michael Tacke
CPC分类号： C12N9/0006 , C12N9/54 , C12Q1/54 , C12Y101/03004 , G01N2333/904
摘要： Novel glucose oxidase (GOx) variants are disclosed that have the substitutions of T30V and I94V set forth in SEQ ID NO:1, and additionally at least one further amino acid substitution in the enzyme sequence in any of the positions S53; A137; A173; A332; F414 and V560. The GOx variants herein exhibit specificity for glucose and significantly reduced oxygen consumption rates and/or increased enzyme activity for electron mediators other than oxygen. Also provided are assay devices incorporating at least one of the GOx variants herein for improved blood glucose measurements.
-
公开(公告)号：US09399790B2
公开(公告)日：2016-07-26
申请号：US14222645
申请日：2014-03-23
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32
摘要： The present invention provides for stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives of formula (I), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits.
摘要翻译： 本发明提供式（I）的稳定的烟酰胺腺嘌呤二核苷酸（NAD / NADH）和烟酰胺腺嘌呤二核苷酸磷酸（NADP / NADPH）衍生物，这些衍生物的酶复合物及其在生物化学检测方法和试剂盒中的用途。
-
公开(公告)号：US11220674B2
公开(公告)日：2022-01-11
申请号：US15868055
申请日：2018-01-11
摘要： The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes.
-
公开(公告)号：US09840729B2
公开(公告)日：2017-12-12
申请号：US14226884
申请日：2014-03-27
发明人： Stacy H. Duvall , Peter Gebauer , Dieter Heindl , Carina Horn , Peter Kratzsch , Michael Marquant , Thomas Meier
IPC分类号： C12Q1/54 , C07D295/215 , C07C281/20
CPC分类号： C12Q1/54 , C07C281/20 , C07D295/215
摘要： Azo compounds are disclosed that do not form azoxy dimers and that do not have any reactive nitroso groups. Also disclosed are use of the azo compounds as mediators in optical and electrochemical diagnostic methods, as well as detection reagents, kits and test elements that include such azo compounds and that can be used in the diagnostic methods.
-
公开(公告)号：US20160326567A1
公开(公告)日：2016-11-10
申请号：US15214494
申请日：2016-07-20
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32
摘要： The present invention provides for stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives of formula (I), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits.
摘要翻译： 本发明提供式（I）的稳定的烟酰胺腺嘌呤二核苷酸（NAD / NADH）和烟酰胺腺嘌呤二核苷酸磷酸（NADP / NADPH）衍生物，这些衍生物的酶复合物及其在生物化学检测方法和试剂盒中的用途。
-
公开(公告)号：US20180265849A1
公开(公告)日：2018-09-20
申请号：US15868055
申请日：2018-01-11
CPC分类号： C12N9/0006 , C12N9/96
摘要： The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes.
-
公开(公告)号：US20170073661A1
公开(公告)日：2017-03-16
申请号：US15363038
申请日：2016-11-29
发明人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
CPC分类号： C12N9/96 , C12N9/0006 , C12N9/0036 , C12Q1/26 , C12Q1/32 , C12Y101/01001 , C12Y101/01002 , C12Y101/01047 , C12Y101/01049 , C12Y106/05002 , C12Y106/99
摘要： Methods are provided for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme. In addition, enzymes are provided that are stabilized with a stabilized coenzyme, as well as the use thereof in test elements for detecting analytes. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
摘要翻译： 提供了通过在稳定的辅酶的存在下储存酶来稳定酶的方法。 此外，提供了用稳定化的辅酶稳定的酶以及其用于检测分析物的测试元件中的用途。 其他方面包括独特的组合物，方法，技术，涉及酶稳定化的系统和装置。
-
9.发明申请Glucose Oxidase Variants, As Well As Compositions, Devices, Kits and Uses Thereof 有权葡萄糖氧化酶变体，以及组合物，设备，试剂盒及其用途公开(公告)号：US20160002609A1
公开(公告)日：2016-01-07
申请号：US14810070
申请日：2015-07-27
发明人： Marco Bocola , Hartmut Duefel , Erik Uwe Gutierrez , Dieter Heindl , Thomas Meier , Hemanshu Mundhada , Ulrich Schwaneberg , Michael Tacke
CPC分类号： C12N9/0006 , C12N9/54 , C12Q1/54 , C12Y101/03004 , G01N2333/904
摘要： Novel glucose oxidase variants are disclosed that have besides the two substitutions T30V and I94V according to SEQ ID NO:1, additionally at least one further amino acid substitution in the polypeptide sequence in any of the positions S53; A137; A173; A332; F414 and V560. The glucose oxidase variants herein exhibit specificity for glucose and significantly reduced oxygen consumption rates and/or increased enzyme activity for electron mediators other than oxygen. Also provided are assay devices incorporating at least one of the GOx variants herein for improved blood glucose measurements.
摘要翻译： 公开了新的葡萄糖氧化酶变体，除了根据SEQ ID NO：1的两个取代T30V和I94V之外，另外在任何位置S53中的多肽序列中至少另外一个氨基酸取代; A137; A173; A332; F414和V560。 本文中的葡萄糖氧化酶变体表现出对于除氧之外的电子介体对葡萄糖的特异性和显着降低的氧消耗速率和/或增加的酶活性。 还提供了结合至少一种GOx变体的测定装置用于改善血糖测量。
-
-
-
-
-
-
-
-
搜索结果
9 条记录 (耗时 0.017 秒)
