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公开(公告)号：US20200263248A1
公开(公告)日：2020-08-20
申请号：US16822802
申请日：2020-03-18
IPC分类号： C12Q1/6874 , G01N33/487 , G01N27/447 , C07H19/10
摘要： The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5′-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.
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公开(公告)号：US20170342485A1
公开(公告)日：2017-11-30
申请号：US15604611
申请日：2017-05-24
发明人： Dmitriy Gremyachinskiy , Meng Taing , Aruna Ayer , Peter Crisalli
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C07H19/10 , G01N27/44791 , G01N33/48721 , C12Q2525/313 , C12Q2535/122 , C12Q2563/116 , C12Q2565/631
摘要： The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5′-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.
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公开(公告)号：US20240150824A1
公开(公告)日：2024-05-09
申请号：US18041348
申请日：2021-08-09
发明人： Aruna Ayer , Frank Bergmann , Peter Crisalli , Lars Hillringhaus , Omid Khakshoor , Hannes Kuchelmeister , Rongxin Nie , Marshall W. Porter , Christoph Seidel , Seong-Ho Shin , Meng Taing , Adolfo Vargas
IPC分类号： C12Q1/6869 , B01L3/00 , C12Q1/6876
CPC分类号： C12Q1/6869 , B01L3/50273 , C12Q1/6876 , B01L2200/0694 , B01L2200/16 , B01L2300/0645 , B01L2400/0418
摘要： The present disclosure relates to tagged nucleoside-5′-oligophosphates having a positively charged polymer tag structure and components thereof. Such nucleoside-5′-oligophosphates are useful, for example, in nanopore-based sequencing-by-synthesis applications. Also disclosed herein are nanopore constructs engineered to have additional negatively-charged moieties in the channel of the nanopore. Such nanopores can be useful, for example, for providing a repellant force against template and/or primer nucleic acids inserting into the pore during a nucleic sequence-by-synthesis process. The tagged nucleoside-5′-oligophosphates and nanopores disclosed herein can be used together to provide nanopore-based nucleic acid sequencing-by-synthesis systems and processes having reduced background tag levels and improved throughput.
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公开(公告)号：US20210292832A1
公开(公告)日：2021-09-23
申请号：US17181679
申请日：2021-02-22
IPC分类号： C12Q1/6874 , C07H19/10 , G01N27/447 , G01N33/487
摘要： The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5′-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.
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公开(公告)号：US10975432B2
公开(公告)日：2021-04-13
申请号：US16822802
申请日：2020-03-18
IPC分类号： C12Q1/68 , C12Q1/6874 , C07H19/10 , G01N27/447 , G01N33/487
摘要： The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5′-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.
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公开(公告)号：US11773130B2
公开(公告)日：2023-10-03
申请号：US17858099
申请日：2022-07-06
发明人： Frank Bergmann , Peter Crisalli , Dieter Heindl , Omid Khakshoor , Meng Taing
IPC分类号： C07H19/06 , C07H19/048
CPC分类号： C07H19/06 , C07H19/048
摘要： The present disclosure provides 3′ protected nucleotides, including those 3′ protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3′ protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.
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公开(公告)号：US20230159999A1
公开(公告)日：2023-05-25
申请号：US17817480
申请日：2022-08-04
发明人： Aruna Ayer , David Beyer , Peter Crisalli , Kirti Dhiman , Helen Franklin , Swathi Chakravarthy , Omid Khakshoor , Blake Langdon , Meng Taing , Adolfo Vargas
IPC分类号： C12Q1/6869
CPC分类号： C12Q1/6869
摘要： This application discloses compositions comprising primer compounds that reduce or block deleterious threading into a nanopore of nucleic acid strands displaced by a nanopore-linked polymerase, for example during the use of a nanopore device for nucleic acid sequencing. Also disclosed are methods for using the compositions to reduce deleterious threading events during nanopore-based nucleic acid detection techniques, such as nanopore sequencing.
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公开(公告)号：US20220348604A1
公开(公告)日：2022-11-03
申请号：US17858099
申请日：2022-07-06
发明人： Frank Bergmann , Peter Crisalli , Dieter Hiendl , Omid Khakshoor , Meng Taing
IPC分类号： C07H19/06 , C07H19/048
摘要： The present disclosure provides 3′ protected nucleotides, including those 3′ protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3′ protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.
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公开(公告)号：US20240010668A1
公开(公告)日：2024-01-11
申请号：US18233265
申请日：2023-08-11
发明人： Frank Bergmann , Peter Crisalli , Dieter Heindl , Omid Khakshoor , Meng Taing
IPC分类号： C07H19/06 , C07H19/048
CPC分类号： C07H19/06 , C07H19/048
摘要： The present disclosure provides 3′ protected nucleotides, including those 3′ protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3′ protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.
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公开(公告)号：US11440933B2
公开(公告)日：2022-09-13
申请号：US17416186
申请日：2019-12-16
发明人： Frank Bergmann , Peter Crisalli , Dieter Hiendl , Omid Khakshoor , Meng Taing
IPC分类号： C07H19/06 , C07H19/048
摘要： The present disclosure provides 3′ protected nucleotides, including those 3′ protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3′ protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.
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