-
公开(公告)号：US5194601A
公开(公告)日：1993-03-16
申请号：US409843
申请日：1989-09-18
IPC分类号： C12N15/38 , C12N15/869
CPC分类号： C12N15/86 , C12N2710/16243
摘要： The invention provides lymphotrophic herpes virus (preferably EBV) recombinants. The portions of the virus that are responsible for the packaging and lytic phase replication have been isolated and cloned. When used with vectors and hosts containing a segment that controls plasmid replication, they provide a means of carrying foreign genes into B-lymphocytes.
摘要翻译： 本发明提供淋巴细胞性疱疹病毒（优选EBV）重组体。 负责包装和裂解相复制的病毒部分已被分离并克隆。 当与含有控制质粒复制的区段的载体和宿主一起使用时，它们提供将外来基因携带到B淋巴细胞中的方法。
-
公开(公告)号：US4686186A
公开(公告)日：1987-08-11
申请号：US654780
申请日：1984-09-26
申请人： William M. Sugden
发明人： William M. Sugden
IPC分类号： C12N9/72 , C12N15/869 , C12P21/00 , C12N7/00 , C12N15/00
CPC分类号： C12N15/85 , C12N9/6456 , C12N2710/16222 , C12N2710/16243 , C12N2800/108
摘要： A recombinant vector and a eukaryotic host transformed thereby are disclosed. In one embodiment, there is a eukaryotic host that has a recombinant plasmid comprising a plasmid backbone, a foreign eukaryotic gene component, and a first gene segment from a lymphotrophic herpes virus. There is also provided in the host a second gene segment from a lymphotrophic herpes virus. The first and second gene segments interact to assist the plasmid in maintaining itself as a plasmid when inserted into a eukaryotic host, and permit stable replication of the host. The preferred virus is the Epstein-Barr virus, and the two preferred viral segments are the EBV origin of replication and the segment coding for EBNA protein.
摘要翻译： 公开了由此转化的重组载体和真核宿主。 在一个实施方案中，存在具有包含质粒骨架，外源真核基因组分和来自淋巴管疱疹病毒的第一基因区段的重组质粒的真核宿主。 在宿主中还提供了来自淋巴细胞性疱疹病毒的第二基因片段。 当插入到真核宿主中时，第一和第二基因片段相互作用以帮助质粒保持其自身为质粒，并允许宿主的稳定复制。 优选的病毒是爱泼斯坦 - 巴尔病毒，并且两个优选的病毒片段是EBV复制起点和编码EBNA蛋白的片段。
-
公开(公告)号：US09206439B2
公开(公告)日：2015-12-08
申请号：US12353835
申请日：2009-01-14
CPC分类号： C12N15/85 , C12N2710/16222 , C12N2820/60
摘要： The invention provides a recombinant vector comprising a DNA segment having a synthetic origin of DNA synthesis that binds EBNA-1 and is capable of initiating DNA synthesis of sequences linked to the synthetic origin of DNA synthesis and maintaining the linked sequences when in the presence of EBNA-1. The synthetic origin of DNA synthesis comprises at least two binding sites for EBNA-1, wherein the two EBNA-1 binding sites are flanked by at least two half-binding sites for TRF2 or at least two binding sites for a protein that enhances the affinity of EBNA-1 for the synthetic origin of DNA synthesis. Further provided are host cells with the vector and methods of using the vector, for instance, ex vivo or in vivo.
摘要翻译： 本发明提供一种重组载体，其包含具有结合EBNA-1的合成DNA合成起点的DNA区段，并且能够启动与合成DNA合成起始点连接的序列的DNA合成并在EBNA存在时维持连接序列 -1。 DNA合成的合成起点包含至少两个EBNA-1的结合位点，其中两个EBNA-1结合位点侧接于TRF2的至少两个半结合位点或增强亲和力的蛋白质的至少两个结合位点 的EBNA-1用于DNA合成的合成来源。 还提供了具有载体的宿主细胞和使用载体的方法，例如离体或体内。
-
公开(公告)号：US07465580B2
公开(公告)日：2008-12-16
申请号：US10848976
申请日：2004-05-19
IPC分类号： C12N15/00 , C12N7/08 , C12N5/06 , A61K39/395 , A61K39/245
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N2710/16222 , C12N2710/16243 , C12N2840/203
摘要： The invention provides a vector encoding a derivative of EBNA-1 that is not cytotoxic when expressed efficiently in cells, which supports extrachromosomal replication, maintenance and transcription from extrachromosomal oriP containing vectors but does not substantially activate transcription from host cell genes. Also provided is a vector having oriP and encoding a derivative of EBNA-1. The vectors of the invention may be employed in vitro and in gene therapy.
摘要翻译： 本发明提供编码EBNA-1衍生物的载体，当在细胞中有效表达时，其不具有细胞毒性，其支持来自染色体外含oriP的载体的染色体外复制，维持和转录，但基本上不激活宿主细胞基因的转录。 还提供了具有oriP并编码EBNA-1的衍生物的载体。 本发明的载体可以在体外和基因治疗中使用。
-
公开(公告)号：US20090203545A1
公开(公告)日：2009-08-13
申请号：US12353835
申请日：2009-01-14
CPC分类号： C12N15/85 , C12N2710/16222 , C12N2820/60
摘要： The invention provides a recombinant vector comprising a DNA segment having a synthetic origin of DNA synthesis that binds EBNA-1 and is capable of initiating DNA synthesis of sequences linked to the synthetic origin of DNA synthesis and maintaining the linked sequences when in the presence of EBNA-1. The synthetic origin of DNA synthesis comprises at least two binding sites for EBNA-1, wherein the two EBNA-1 binding sites are flanked by at least two half-binding sites for TRF2 or at least two binding sites for a protein that enhances the affinity of EBNA-1 for the synthetic origin of DNA synthesis. Further provided are host cells with the vector and methods of using the vector, for instance, ex vivo or in vivo.
摘要翻译： 本发明提供一种重组载体，其包含具有结合EBNA-1的合成DNA合成起点的DNA区段，并且能够启动与合成DNA合成起始点连接的序列的DNA合成并在EBNA存在时维持连接序列 -1。 DNA合成的合成起点包含至少两个EBNA-1的结合位点，其中两个EBNA-1结合位点侧接于TRF2的至少两个半结合位点或增强亲和力的蛋白质的至少两个结合位点 的EBNA-1用于DNA合成的合成来源。 还提供了具有载体的宿主细胞和使用载体的方法，例如离体或体内。
-
公开(公告)号：US06960429B2
公开(公告)日：2005-11-01
申请号：US09808517
申请日：2001-03-14
申请人： William M. Sugden , David M. Mackey
发明人： William M. Sugden , David M. Mackey
CPC分类号： G01N33/502 , A61K38/00 , C07K14/005 , C12N2710/16222 , G01N33/5008 , G01N2333/05
摘要： A method of inhibiting viral gene activities (and cellular gene activities) is disclosed. In one embodiment, the method comprises the step of delivering an effective amount of an inhibitor of a viral looping/linking factor to an infected patient.
摘要翻译： 公开了抑制病毒基因活性（和细胞基因活性）的方法。 在一个实施方案中，该方法包括将有效量的病毒环化/连接因子抑制剂递送至感染患者的步骤。
-
公开(公告)号：US06811983B2
公开(公告)日：2004-11-02
申请号：US10201887
申请日：2002-07-24
IPC分类号： C12Q168
CPC分类号： C12Q1/18 , G01N2333/05 , G01N2500/04 , G01N2500/10 , G01N2510/00
摘要： A method of assaying potential inhibitors of EBNA-1 is disclosed. In one aspect, this invention comprises the steps of (a) obtaining an EBV-positive cell line and an EBV-negative cell line; (b) exposing the cell lines to a test compound, and (c) observing the effect of the compound on induction of apoptosis in the cell lines, wherein significant apoptosis in the EBV-positive cell line and lack of significant apoptosis in the EBV-negative cell line indicates a compound that specifically inhibits EBNA-1.
摘要翻译： 公开了一种测定EBNA-1的潜在抑制剂的方法。 一方面，本发明包括以下步骤：（a）获得EBV阳性细胞系和EBV阴性细胞系; （b）将细胞系暴露于测试化合物，（c）观察化合物对细胞系凋亡诱导的作用，其中EBV阳性细胞系中的细胞凋亡和EBV- 阴性细胞系表示特异性抑制EBNA-1的化合物。
-
-
-
-
-
-
搜索结果
7 条记录 (耗时 0.019 秒)
