公开(公告)号：US20050153447A1

公开(公告)日：2005-07-14

申请号：US11001921

申请日：2004-12-02

申请人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Chana

发明人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Chana

IPC分类号： C12M1/00 ,  A61K35/12 ,  A61K39/00 ,  A61L27/38 ,  C07K16/28 ,  C12M3/00 ,  C12N5/0781 ,  C12N5/0783 ,  C12N5/0789 ,  C12N5/08

CPC分类号： A61L27/3804 ,  A61K2035/124 ,  A61K2039/5158 ,  A61K2039/57 ,  C07K16/2803 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2821 ,  C07K16/2827 ,  C07K16/2833 ,  C07K16/2845 ,  C07K16/2854 ,  C07K16/2866 ,  C07K16/2875 ,  C07K16/2878 ,  C07K16/289 ,  C07K16/2896 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0647 ,  C12N2500/32 ,  C12N2501/23 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/53 ,  C12N2501/59 ,  C12N2533/12 ,  C12N2533/14 ,  C12N2533/18 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/90

摘要： The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

公开(公告)号：US20050214942A1

公开(公告)日：2005-09-29

申请号：US10984351

申请日：2004-11-08

申请人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Chana

发明人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Chana

IPC分类号： C12M1/00 ,  A61K35/12 ,  A61K39/00 ,  A61L27/38 ,  C07K16/28 ,  C12M3/00 ,  C12N5/0781 ,  C12N5/0783 ,  C12N5/0789 ,  C12N5/08

CPC分类号： A61L27/3804 ,  A61K2035/124 ,  A61K2039/5158 ,  A61K2039/57 ,  C07K16/2803 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2821 ,  C07K16/2827 ,  C07K16/2833 ,  C07K16/2845 ,  C07K16/2854 ,  C07K16/2866 ,  C07K16/2875 ,  C07K16/2878 ,  C07K16/289 ,  C07K16/2896 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0647 ,  C12N2500/32 ,  C12N2501/23 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/53 ,  C12N2501/59 ,  C12N2533/12 ,  C12N2533/14 ,  C12N2533/18 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/90

摘要： The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

摘要翻译： 本发明一般涉及用于激活和扩增细胞的方法，更具体地，涉及一种激活和/或刺激细胞的新方法，其使这种细胞的扩增最大化以达到显着高的密度。 在各种实施例中，细胞被激活并在短时间内扩展到非常高的密度。 在某些实施方案中，细胞在短时间内被激活并扩增至非常高数量的细胞。 进一步提供通过本文方法激活和扩增的细胞的组成。

公开(公告)号：US06867041B2

公开(公告)日：2005-03-15

申请号：US10133236

申请日：2002-04-26

申请人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen

发明人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen

IPC分类号： A61K35/12 ,  A61K39/00 ,  A61L27/38 ,  C07K16/28 ,  C12N5/0781 ,  C12N5/0783 ,  C12N5/0789 ,  C12N5/00

CPC分类号： C12N5/0647 ,  A61K2035/124 ,  A61K2039/57 ,  A61L27/38 ,  C07K16/2803 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2821 ,  C07K16/2827 ,  C07K16/2833 ,  C07K16/2845 ,  C07K16/2854 ,  C07K16/2866 ,  C07K16/2875 ,  C07K16/2878 ,  C07K16/289 ,  C07K16/2896 ,  C12N5/0635 ,  C12N5/0636 ,  C12N2500/32 ,  C12N2501/23 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/53 ,  C12N2533/12 ,  C12N2533/14 ,  C12N2533/18 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/90

摘要： The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and/or stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

摘要翻译： 本发明一般涉及刺激细胞的方法，更具体地，涉及一种浓缩和/或刺激使这种细胞的刺激和/或增殖最大化的细胞的新方法。 在各种实施方案中，刺激细胞并用表面产生增强的增殖，细胞信号转导和/或细胞表面部分聚集来浓缩细胞。 在某些方面，通过使细胞群与表面连接，通过同时浓缩和细胞表面部分连接来刺激诸如T细胞的细胞群体的方法，其中连接有一种或多种连接细胞表面部分的试剂 并施加主要驱动细胞浓度和细胞表面部分连接的力，从而诱导细胞刺激，细胞表面部分聚集和/或受体信号增强。 还提供了用于产生表型定制细胞的方法，包括用于诊断，药物发现和治疗各种适应症（包括癌症，病毒感染和免疫相关疾病）的T细胞。 还提供了由这些方法产生的具有特定表现性质的细胞的组成。

公开(公告)号：US06905874B2

公开(公告)日：2005-06-14

申请号：US09794230

申请日：2001-02-26

申请人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen

发明人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen

IPC分类号： G01N33/53 ,  A61K35/12 ,  A61K35/14 ,  A61K39/00 ,  A61L27/38 ,  A61P35/00 ,  C07K16/28 ,  C12N5/0781 ,  C12N5/0783 ,  C12N5/0789 ,  C12N5/00

CPC分类号： A61L27/3804 ,  A61K2035/124 ,  A61K2039/57 ,  A61L27/3895 ,  C07K16/2803 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2821 ,  C07K16/2827 ,  C07K16/2833 ,  C07K16/2845 ,  C07K16/2854 ,  C07K16/2866 ,  C07K16/2875 ,  C07K16/2878 ,  C07K16/289 ,  C07K16/2896 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0647 ,  C12N2500/32 ,  C12N2501/23 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/53 ,  C12N2533/12 ,  C12N2533/14 ,  C12N2533/18 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/90

摘要： The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

摘要翻译： 本发明一般涉及刺激细胞的方法，更具体地，涉及一种浓缩和刺激使这种细胞的刺激和/或增殖最大化的细胞的新方法。 在各种实施方案中，刺激细胞并用表面产生增强的增殖，细胞信号转导和/或细胞表面部分聚集来浓缩细胞。 在某些方面，通过使细胞群与表面连接，通过同时浓缩和细胞表面部分连接来刺激诸如T细胞的细胞群体的方法，其中附着有一种或多种连接细胞表面部分的试剂 并施加主要驱动细胞浓度和细胞表面部分连接的力，从而诱导细胞刺激，细胞表面部分聚集和/或受体信号增强。 还提供了用于产生表型定制细胞的方法，包括用于诊断，药物发现和治疗各种适应症（包括癌症，病毒感染和免疫相关疾病）的T细胞。 还提供了由这些方法产生的具有特定表现性质的细胞的组成。

公开(公告)号：US20060121005A1

公开(公告)日：2006-06-08

申请号：US11070841

申请日：2005-03-02

申请人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Zahbea Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Chana

发明人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Zahbea Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Chana

IPC分类号： A61K39/395 ,  C12N5/08 ,  A61K35/14

CPC分类号： C12N5/0636 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2533/00

摘要： The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

公开(公告)号：US06797514B2

公开(公告)日：2004-09-28

申请号：US09960264

申请日：2001-09-20

申请人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen

发明人： Ronald Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen

IPC分类号： C12N500

CPC分类号： A61L27/3804 ,  A61K2035/124 ,  A61K2039/57 ,  A61L27/3895 ,  C07K16/2803 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2821 ,  C07K16/2827 ,  C07K16/2833 ,  C07K16/2845 ,  C07K16/2854 ,  C07K16/2866 ,  C07K16/2875 ,  C07K16/2878 ,  C07K16/289 ,  C07K16/2896 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0647 ,  C12N2500/32 ,  C12N2501/23 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/53 ,  C12N2533/12 ,  C12N2533/14 ,  C12N2533/18 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/90

摘要： The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

摘要翻译： 本发明一般涉及刺激细胞的方法，更具体地，涉及一种浓缩和刺激使这种细胞的刺激和/或增殖最大化的细胞的新方法。 在各种实施方案中，刺激细胞并用表面产生增强的增殖，细胞信号转导和/或细胞表面部分聚集来浓缩细胞。 在某些方面，通过使细胞群与表面连接，通过同时浓缩和细胞表面部分连接来刺激诸如T细胞的细胞群体的方法，其中连接有一种或多种连接细胞表面部分的试剂 并施加主要驱动细胞浓度和细胞表面部分连接的力，从而诱导细胞刺激，细胞表面部分聚集和/或受体信号增强。 还提供了用于产生表型定制细胞的方法，包括用于诊断，药物发现和治疗各种适应症（包括癌症，病毒感染和免疫相关疾病）的T细胞。 还提供了由这些方法产生的具有特定表现性质的细胞的组成。

公开(公告)号：US07541184B2

公开(公告)日：2009-06-02

申请号：US10762210

申请日：2004-01-20

申请人： Ronald J. Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Singh Chana

发明人： Ronald J. Berenson ,  Che Law ,  Mark Bonyhadi ,  Narinder Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Singh Chana

IPC分类号： C12N5/00

CPC分类号： A61L27/3804 ,  A61K2035/124 ,  A61K2039/5158 ,  A61K2039/57 ,  A61L27/3895 ,  C07K16/2803 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2821 ,  C07K16/2827 ,  C07K16/2833 ,  C07K16/2845 ,  C07K16/2854 ,  C07K16/2866 ,  C07K16/2875 ,  C07K16/2878 ,  C07K16/289 ,  C07K16/2896 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0647 ,  C12N2500/32 ,  C12N2501/23 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/53 ,  C12N2501/59 ,  C12N2533/12 ,  C12N2533/14 ,  C12N2533/18 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/90

摘要： The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

摘要翻译： 本发明一般涉及用于激活和扩增细胞的方法，更具体地，涉及一种激活和/或刺激细胞的新方法，其使这种细胞的扩增最大化以达到显着高的密度。 在各种实施例中，细胞被激活并在短时间内扩展到非常高的密度。 在某些实施方案中，细胞在短时间内被激活并扩增至非常高数量的细胞。 进一步提供通过本文方法激活和扩增的细胞的组成。

公开(公告)号：US07572631B2

公开(公告)日：2009-08-11

申请号：US11070841

申请日：2005-03-02

申请人： Ronald J Berenson ,  Che Law ,  Mark Bonyhadi ,  Zahbea Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Singh Chana

发明人： Ronald J Berenson ,  Che Law ,  Mark Bonyhadi ,  Zahbea Saund ,  Stewart Craig ,  Alan Hardwick ,  Dale Kalamasz ,  David McMillen ,  Harjinder Singh Chana

IPC分类号： C12N5/00

CPC分类号： C12N5/0636 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2533/00

摘要： The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

摘要翻译： 本发明一般涉及用于激活和扩增细胞的方法，更具体地，涉及一种激活和/或刺激细胞的新方法，其使这种细胞的扩增最大化以达到显着高的密度。 在各种实施例中，细胞被激活并在短时间内扩展到非常高的密度。 在某些实施方案中，细胞在短时间内被激活并扩增至非常高数量的细胞。 进一步提供通过本文方法激活和扩增的细胞的组成。

公开(公告)号：US20050084967A1

公开(公告)日：2005-04-21

申请号：US10900046

申请日：2004-07-27

申请人： Ronald Berenson ,  Mark Bonyhadi ,  Dale Kalamasz

发明人： Ronald Berenson ,  Mark Bonyhadi ,  Dale Kalamasz

IPC分类号： A61K35/14 ,  A61K35/17 ,  C12N5/00 ,  C12N5/0783 ,  C12Q1/68 ,  C12N5/08

CPC分类号： C12N5/0087 ,  A61K35/17 ,  C12N5/0636 ,  C12N2501/48 ,  C12N2502/1114 ,  Y02A50/475

摘要： The present invention relates generally to methods for stimulating T cells, and more particularly, to methods to eliminate undesired (e.g. autoreactive, alloreactive, pathogenic) subpopulations of T cells from a mixed population of T cells, thereby restoring the normal immune repertoire of said T cells. The present invention also relates to compositions of cells, including stimulated T cells having restored immune repertoire and uses thereof.

摘要翻译： 本发明一般涉及用于刺激T细胞的方法，更具体地，涉及从T细胞的混合群体中消除T细胞的不需要的（例如自身反应，同种异体反应，致病）亚群的方法，从而恢复所述T细胞的正常免疫谱 细胞。 本发明还涉及细胞的组合物，包括具有恢复的免疫谱的刺激的T细胞及其用途。

公开(公告)号：US07977095B2

公开(公告)日：2011-07-12

申请号：US12251224

申请日：2008-10-14

申请人： Mark Bonyhadi ,  Dale Kalamasz

发明人： Mark Bonyhadi ,  Dale Kalamasz

IPC分类号： C12N5/08 ,  C12N5/00

CPC分类号： C12N5/0636 ,  A61K2035/122 ,  A61K2035/124 ,  A61K2039/515 ,  A61K2039/5158 ,  C12N2501/515 ,  C12N2501/58 ,  C12N2501/599

摘要： The present invention relates generally to methods for generating, expanding, and isolating antigen-specific T cells. Compositions of antigen-specific T cells activated and expanded by the methods herein are further provided.

摘要翻译： 本发明一般涉及产生，扩增和分离抗原特异性T细胞的方法。 进一步提供通过本文方法活化和扩增的抗原特异性T细胞的组合物。