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    Micro and nano scale structures disposed in a material so as to present micrometer and nanometer scale curvature and stiffness patterns for use in cell and tissue culturing and in other surface and interface applications
    2.
    发明授权
    Micro and nano scale structures disposed in a material so as to present micrometer and nanometer scale curvature and stiffness patterns for use in cell and tissue culturing and in other surface and interface applications 有权
    微型和纳米尺度结构设置在材料中，以便呈现用于细胞和组织培养以及其他表面和界面应用中的千分尺和纳米级曲率和刚度图案

    公开(公告)号：US09512397B2

    公开(公告)日：2016-12-06

    申请号：US14456833

    申请日：2014-08-11

    申请人： Shengyuan Yang

    发明人： Shengyuan Yang

    IPC分类号： C12N5/077 ,  C12N5/00

    CPC分类号： C12N5/0068 ,  C12N2533/12 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2535/10

    摘要： A structure for use in cell and tissue culturing and in other surface and interface applications. The structure comprises a first material layer defining one or more surface features therein disposed randomly or in a pattern, the one or more surface features having the same or different sizes and cross sectional shapes, a second material layer disposed in or on the one or more surface features, a microstructure disposed in or on the one or more surface features and at least partially embedded and immobile within the second material layer, the microstructure presenting a curvature and a stiffness value and protruding above an upper surface of the second material, a size of the microstructure between 1 nanometer and 10 millimeters, and the structure for use in cell and tissue culturing and in other surface and interface applications wherein a cell grows on the microstructure.

    摘要翻译： 用于细胞和组织培养以及其他表面和界面应用的结构。 该结构包括限定一个或多个表面特征的第一材料层，其中随机或以图案布置，所述一个或多个表面特征具有相同或不同的尺寸和横截面形状，第二材料层设置在一个或多个 表面特征，布置在所述一个或多个表面特征中或所述第一材料层中并且至少部分地嵌入和固定在所述第二材料层内的微结构，所述微结构呈现出曲率和刚度值并突出在所述第二材料的上表面上方的尺寸 的微结构在1纳米和10毫米之间，以及用于细胞和组织培养的结构以及其中细胞生长在微结构上的其它表面和界面应用中。

    Composition and Method for Delivery of Living Cells in a Dry Mode Having a Surface Layer
    3.
    发明申请
    Composition and Method for Delivery of Living Cells in a Dry Mode Having a Surface Layer 审中-公开
    用具有表面层的干燥模式递送活细胞的组合物和方法

    公开(公告)号：US20160194603A1

    公开(公告)日：2016-07-07

    申请号：US15066956

    申请日：2016-03-10

    申请人： Drylet LLC

    发明人： Ramiro Trevino ,  Steven R. Ellis

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0068 ,  A01N1/0231 ,  C12N5/0602 ,  C12N2533/10 ,  C12N2533/12

    摘要： The present invention generally relates to compositions and methods of delivering living cells in a dry mode, wherein the compositions include a surface layer disposed on the outer surface of the composition that is permeable to carbon dioxide and oxygen. The compositions may be used to deliver living cells to a delivery point without the use of expensive refrigerants such as dry ice or liquid nitrogen.

    摘要翻译： 本发明一般涉及以干燥方式递送活细胞的组合物和方法，其中组合物包括设置在组合物的外表面上的表面层，其可透过二氧化碳和氧气。 组合物可以用于将活细胞递送到递送点，而不使用昂贵的制冷剂，例如干冰或液氮。

    Three dimensional cell culture compositions and methods of use
    4.
    发明授权
    Three dimensional cell culture compositions and methods of use 有权
    三维细胞培养组合物和使用方法

    公开(公告)号：US09334473B2

    公开(公告)日：2016-05-10

    申请号：US13962403

    申请日：2013-08-08

    申请人： Jelena Vukasinovic

    发明人： Jelena Vukasinovic

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0062 ,  C12N5/0068 ,  C12N2533/12 ,  C12N2533/30 ,  C12N2533/90

    摘要： Described herein is a three-dimensional cell culture scaffold composition comprising an absorbent rigid (AR) component, and in some embodiments, further comprises a gel component. The absorbent rigid component preferably comprises a glass fiber material. It is a surprising finding of the present invention that an AR component having a void volume of between approximately 70% and 95% results in a three-dimensional cell culture composition that allows for robust, high-throughput screening and high-content screening accessible tissue models with preserved cell morphology, heterogeneity of cell types and cell populations, extracellular matrix constituents, functional cell-cell and cell-extracellular matrix interactions and signaling with sufficient specificities to tissue physiology and pathology.

    摘要翻译： 本文描述的是包含吸收性刚性（AR）组分的三维细胞培养支架组合物，并且在一些实施方案中还包含凝胶组分。 吸收性刚性部件优选地包括玻璃纤维材料。 本发明令人惊奇的发现是具有大约70％至95％的空隙体积的AR组分导致三维细胞培养组合物，其允许稳健，高通量筛选和高含量筛选可接近的组织 具有保留的细胞形态，细胞类型和细胞群的异质性，细胞外基质成分，功能性细胞和细胞 - 细胞外基质相互作用以及对组织生理学和病理学具有足够特异性的信号的模型。

    Generation of Dendritic Cells from Monocytic Dendritic Precursor Cells with GM-CSF in the Absence of Additional Cytokines
    5.
    发明申请
    Generation of Dendritic Cells from Monocytic Dendritic Precursor Cells with GM-CSF in the Absence of Additional Cytokines 审中-公开
    在没有其他细胞因子的情况下用GM-CSF从单核细胞树突细胞前体细胞中产生树突状细胞

    公开(公告)号：US20160024472A1

    公开(公告)日：2016-01-28

    申请号：US14814315

    申请日：2015-07-30

    申请人： Northwest Biotherapeutics, Inc.

    发明人： Benjamin A. Tjoa ,  Marnix L. Bosch

    IPC分类号： C12N5/0784

    CPC分类号： C12N5/0639 ,  C12N2500/10 ,  C12N2500/30 ,  C12N2500/72 ,  C12N2500/90 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/24 ,  C12N2502/70 ,  C12N2533/12

    摘要： The present invention it was determined that dendritic cells could be derived from various sources including peripheral blood monocytes in the presence of only GM-CSF without other cytokines if the monocytes were not activated. By preventing activation, such as by preventing binding of the cells to the surface of the culture vessel, the monocytes do not require the presence of additional cytokines, such as IL-4 or IL-13, to prevent differentiation into a non-dendritic cell lineage. The immature DCs generated and maintained in this manner were CD14 and expressed high levels of CD1a. Upon maturation by contact with an agent such as, for example, BCG and IFNγ, the cells were determined to express surface molecules typical of mature dendritic cells purified by prior methods and cultured in the presence of GM-CSF and IL-4. The mature dendritic cells produced from monocytes without activation and cultured in GM-CSF alone are suitable for use in dendritic cell-based immunotherapy methods, such as for use in the treatment of disease, including cancer.

    摘要翻译： 本发明确定如果单核细胞未被激活，则在存在只有GM-CSF的情况下，树突状细胞可以来源于各种来源，包括外周血单核细胞。 通过防止活化，例如通过防止细胞与培养容器表面的结合，单核细胞不需要另外的细胞因子如IL-4或IL-13的存在，以防止分化成非树突状细胞 血统。 以这种方式产生和维持的未成熟DCs是CD14并表达高水平的CD1a。 通过与例如BCG和IFNγ的试剂接触成熟，确定细胞表达通过现有方法纯化并在GM-CSF和IL-4存在下培养的成熟树突状细胞的典型表达分子。 由单核细胞产生的成熟树突状细胞没有活化并在单独的GM-CSF中培养，适用于树突状细胞的免疫治疗方法，例如用于治疗包括癌症在内的疾病。

    Cell culture substrate and cell culture method using same
    8.
    发明授权
    Cell culture substrate and cell culture method using same 有权
    细胞培养底物和细胞培养方法使用相同

    公开(公告)号：US09029150B2

    公开(公告)日：2015-05-12

    申请号：US13695779

    申请日：2011-05-10

    申请人： Masaya Nakatani ,  Makoto Takahashi ,  Yoshiki Yamada ,  Takuya Oka

    发明人： Masaya Nakatani ,  Makoto Takahashi ,  Yoshiki Yamada ,  Takuya Oka

    IPC分类号： C12N5/071 ,  C12M3/00 ,  C12M1/12 ,  C12N5/00

    CPC分类号： C12M25/14 ,  C12N5/0068 ,  C12N2533/12 ,  C12N2535/00

    摘要： The present invention provides a cell culture substrate capable of culturing cells efficiently. The cell culture substrate of the present invention includes a substrate, a plurality of fibrous protrusions formed on the substrate, and water-repellent film formed on a surface of each of the fibrous protrusions. The fibrous protrusions are intertwined with each other to form a matrix structure. According to such a cell culture substrate, when a culture solution containing a specimen is discharged to the water-repellent fibrous protrusions, cells can be cultured easily without contact, thus enabling cells to be cultured efficiently.

    摘要翻译： 本发明提供能够有效培养细胞的细胞培养基质。 本发明的细胞培养基材包括基材，形成在基材上的多个纤维状突起，以及形成在各纤维状突起的表面的防水膜。 纤维突起彼此缠结形成矩阵结构。 根据这样的细胞培养基材，当将含有试样的培养液排出到疏水纤维突起时，可以容易地培养细胞而不接触，从而能够有效地培养细胞。

    SILICA-MATRIX FORMING COMPOSITIONS, MATERIALS FORMED THEREFROM, AND METHODS OF USING THE SAME
    9.
    发明申请
    SILICA-MATRIX FORMING COMPOSITIONS, MATERIALS FORMED THEREFROM, AND METHODS OF USING THE SAME 有权
    二氧化硅 - 基质形成组合物，其形成的材料及其使用方法

    公开(公告)号：US20140302130A1

    公开(公告)日：2014-10-09

    申请号：US14355341

    申请日：2012-11-07

    申请人： Eduardo Reategui ,  Lisa Kasinkas ,  Alptekin Aksan

    发明人： Eduardo Reategui ,  Lisa Kasinkas ,  Alptekin Aksan

    IPC分类号： A61K9/48 ,  A61K35/00 ,  A61K35/12

    CPC分类号： A61K9/4816 ,  A61K35/12 ,  A61K35/13 ,  C12N5/0068 ,  C12N11/14 ,  C12N2533/12

    摘要： The present invention provides compositions for encapsulation of biomaterials in a silica-matrix. The present invention also provides methods of making silica-matrix encapsulated biomaterials, and to methods of using silica-matrix encapsulated biomaterials. In one embodiment, the present invention provides a method of encapsulating mammalian cells in a silica-matrix while maintaining metabolic activity. In another embodiment, the present invention provides a method of purifying cancer cells using a silica-matrix.

    摘要翻译： 本发明提供用于将生物材料包封在二氧化硅基质中的组合物。 本发明还提供制备二氧化硅基质包封的生物材料的方法，以及使用二氧化硅 - 基质包封的生物材料的方法。 在一个实施方案中，本发明提供将哺乳动物细胞包封在二氧化硅基质中同时维持代谢活性的方法。 在另一个实施方案中，本发明提供了使用二氧化硅基质纯化癌细胞的方法。

    MICRO AND NANO GLASS BALLS EMBEDDED IN A GEL PRESENTING MICROMETER AND NANOMETER SCALE CURVATURE AND STIFFNESS PATTERNS FOR USE IN CELL AND TISSUE CULTURING AND A METHOD FOR MAKING SAME
    10.
    发明申请
    MICRO AND NANO GLASS BALLS EMBEDDED IN A GEL PRESENTING MICROMETER AND NANOMETER SCALE CURVATURE AND STIFFNESS PATTERNS FOR USE IN CELL AND TISSUE CULTURING AND A METHOD FOR MAKING SAME 有权
    嵌入凝胶微胶粒和纳米尺度曲线和细胞和组织培养中使用的硬度模式的微米和纳米玻璃棒及其制备方法

    公开(公告)号：US20140147918A1

    公开(公告)日：2014-05-29

    申请号：US13656808

    申请日：2012-11-25

    申请人： Shengyuan Yang

    发明人： Shengyuan Yang

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0068 ,  C12N2533/12 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2535/10

    摘要： A substrate for use in culturing cells or tissues. The substrate comprises a gel, one or more microstructures partially embedded within a surface of the gel, the one or more microstructures presenting two different curvatures or presenting two different stiffness values or presenting a combination of different curvatures and different stiffness values, wherein the microstructures are disposed at defined locations within the surface of the gel, and wherein the cells and tissues are cultured on an exposed surface of the microstructures.

    摘要翻译： 用于培养细胞或组织的底物。 基体包括凝胶，部分地嵌入凝胶表面内的一个或多个微结构，所述一个或多个微结构具有两个不同的曲率或呈现两个不同的刚度值或呈现不同曲率和不同刚度值的组合，其中微结构是 设置在凝胶表面内的限定位置，并且其中细胞和组织在微结构的暴露表面上培养。