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    Multiplexed Analyses of Test Samples
    1.
    发明申请
    Multiplexed Analyses of Test Samples 审中-公开
    测试样本的多路复用分析

    公开(公告)号：US20150168388A1

    公开(公告)日：2015-06-18

    申请号：US14569241

    申请日：2014-12-12

    申请人： SomaLogic, Inc.

    发明人： James R. Heil ,  Daniel J. Schneider ,  Daniel T. Nieuwlandt ,  Sheri K. Wilcox ,  Dominic Zichi ,  Todd Gander ,  Bruce Eaton ,  Larry Gold

    IPC分类号： G01N33/543

    摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).

    摘要翻译： 本公开描述了用于检测可能存在于测试样品中的一种或多种靶分子的方法，装置，试剂和试剂盒。 在一个实施方案中，将测试样品与包含标签的适体接触并对靶分子具有特异性亲和力。 允许形成包含与其靶分子结合的适体的适体亲和复合体。 如果测试样品含有目标分子，通常将在测试样品中形成适体亲和复合体。 适体亲和复合物任选地转化为适体共价复合物，其包括与其靶分子共价结合的适体。 然后可以使用本领域技术人员已知的各种方法中的任一种检测和/或定量适体亲和复合物（或任选的适体共价复合物），包括使用固体支持物，使用质谱法和使用定量聚合酶链反应 （Q-PCR）。

    Method for Generating Aptamers with Improved Off-Rates
    6.
    发明申请
    Method for Generating Aptamers with Improved Off-Rates 有权
    用改进的脱离率产生适配器的方法

    公开(公告)号：US20150148237A1

    公开(公告)日：2015-05-28

    申请号：US14606134

    申请日：2015-01-27

    申请人： SomaLogic, Inc.

    发明人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold ,  Thale C. Jarvis ,  Jeffrey D. Carter

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1048 ,  C12N15/115 ,  C12N2310/16 ,  C12Q1/6811 ,  G01N33/5308 ,  C12Q2525/117 ,  C12Q2525/205 ,  C12Q2541/101

    摘要： The present disclosure describes the identification and use of aptamers and photoaptamers having slower dissociation rate constants than those obtained using previously described methods. Specifically, the present disclosure describes methods for the identification and use of aptamers to one or more targets within a histological or cytological sample, which have slow rates of dissociation. The aptamers may be used to assess localization, relative density, and presence or absence of one or more targets in cytological and histological samples. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected. The aptamers may also be used to introduce target specific signal moieties. In addition to target identification, the aptamers may be used to amplify signal generation through a variety of methods.

    摘要翻译： 本公开描述了具有比使用先前描述的方法获得的那些更快的解离速率常数的适配体和光致抗体的鉴定和使用。 具体地，本公开描述了用于鉴定和使用适配体到组织学或细胞学样品中的一种或多种目标的方法，其具有缓慢的解离速率。 适配体可用于评估细胞学和组织学样品中的一种或多种靶标的定位，相对密度和存在或不存在。 可以选择对于样品收集的给定疾病状态的特异性和诊断性的靶标。 适体也可以用于引入目标特异性信号部分。 除目标识别之外，适体可用于通过各种方法放大信号产生。

    Multiplexed analyses of test samples
    7.
    发明授权
    Multiplexed analyses of test samples 有权

    公开(公告)号：US10648972B2

    公开(公告)日：2020-05-12

    申请号：US15878021

    申请日：2018-01-23

    申请人： SomaLogic, Inc.

    发明人： James R. Heil ,  Daniel J. Schneider ,  Daniel T. Nieuwlandt ,  Sheri K. Wilcox ,  Dominic Zichi ,  Todd Gander ,  Bruce Eaton ,  Larry Gold

    IPC分类号： G01N33/543 ,  G01N33/58 ,  C40B40/00 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6832

    摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).

    Multiplexed analyses of test samples
    10.
    发明授权
    Multiplexed analyses of test samples 有权
    测试样品的多重分析

    公开(公告)号：US09404919B2

    公开(公告)日：2016-08-02

    申请号：US14176933

    申请日：2014-02-10

    申请人： SomaLogic, Inc.

    发明人： Daniel J. Schneider ,  Dan Nieuwlandt ,  Bruce Eaton ,  Martin Stanton ,  Shashi Gupta ,  Stephan Kraemer ,  Dominic Zichi ,  Larry Gold

    IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/74

    CPC分类号： G01N33/5308 ,  G01N33/54306 ,  G01N33/6863 ,  G01N33/74

    摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

    摘要翻译： 本公开描述了用于检测可能存在于测试样品中的一种或多种靶分子的方法，装置，试剂和试剂盒。 所描述的方法，装置，试剂盒和试剂通过检测和定量核酸（即，适体）促进了测试样品中非核酸靶标（例如，蛋白质靶标）的检测和定量。 所描述的方法为非核酸靶产生了核酸替代物，从而允许将广泛种类的核酸技术（包括扩增）应用于更广泛的期望目标，特别是蛋白质靶标。 本公开进一步描述了适于在各种分析检测应用中促进适配体使用的适体构建体。