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公开(公告)号：US06747056B2
公开(公告)日：2004-06-08
申请号：US10359145
申请日：2003-02-06
IPC分类号： A61K3140
CPC分类号： C07D207/32
摘要： The present invention comprising culturing a microorganism which belongs to genus Streptomyces and has ability to produce K99-5041-C1x substance and/or K99-5041-C2x substance in a medium, accumulating K99-5041-C1x substance and/or K99-5041-C2x substance in the culture fluid, and isolating K99-5041-C1x substance and/or K99-5041-C2x substance form the cultured mass. Since the obtained K99-5041-C1x substance and/or K99-5041-C2x substance exhibit inhibitory activity against lanosterol synthase, these substances are expected to apply clinically for preventive and therapeutic agents caused by accumulation of cholesterol in humans and antifungal agents.
摘要翻译： 本发明包括培养属于链霉菌属的微生物，并且能够在培养基中产生K99-5041-C1x物质和/或K99-5041-C2x物质，积累K99-5041-C1x物质和/或K99-5041- 培养液中的C2x物质，并从培养物质中分离K99-5041-C1x物质和/或K99-5041-C2x物质。 由于所获得的K99-5041-C1x物质和/或K99-5041-C2x物质对羊毛甾醇合酶表现出抑制活性，所以预期这些物质将临床应用于人类和抗真菌剂中胆固醇积聚引起的预防和治疗剂。
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公开(公告)号：US07575892B2
公开(公告)日：2009-08-18
申请号：US10590661
申请日：2005-02-25
CPC分类号： C12N9/0004 , C12N9/0083 , C12P33/06
摘要： Soyasapogenol B is biosynthesized via two steps of hydroxylation reaction of its precursor β-amyrin. However, the gene of the hydroxylase concerned in this reaction has not been revealed. Therefore, it was impossible to apply a genetic engineering technique on the hydroxylase. The present inventors reveals that a sequence which corresponds to a soybean-derived cytochrome P-450 gene CYP93E1 encodes an enzyme protein that carries out hydroxylation of the 24-position of an oleanane type triterpene, and also provides a method for applying said gene making use of a genetic engineering technique.
摘要翻译： 通过其前体β-二氨基吡啶的两步羟基化反应生物合成大豆皂甙B. 然而，该反应中涉及的羟化酶的基因尚未被揭示。 因此，不可能在羟化酶上应用遗传工程技术。 本发明人发现，与大豆来源的细胞色素P-450基因CYP93E1相对应的序列编码进行炔丙醇型三萜烯的24位的羟基化的酶蛋白，并且还提供了使用所述基因使用的方法 的遗传工程技术。
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公开(公告)号：US20080003639A1
公开(公告)日：2008-01-03
申请号：US10590661
申请日：2005-02-25
CPC分类号： C12N9/0004 , C12N9/0083 , C12P33/06
摘要： Soyasapogenol B is biosynthesized via two steps of hydroxylation reaction of its precursor β-amyrin. However, the gene of the hydroxylase concerned in this reaction has not been revealed. Therefore, it was impossible to apply a genetic engineering technique on the hydroxylase. The present inventors reveals that a sequence which corresponds to a soybean-derived cytochrome P-450 gene CYP93E1 encodes an enzyme protein that carries out hydroxylation of the 24-position of an oleanane type triterpene, and also provides a method for applying said gene making use of a genetic engineering technique.
摘要翻译： 通过其前体β-二氨基吡啶的两步羟基化反应生物合成大豆皂甙B. 然而，该反应中涉及的羟化酶的基因尚未被揭示。 因此，不可能在羟化酶上应用遗传工程技术。 本发明人发现，与大豆来源的细胞色素P-450基因CYP93E1相对应的序列编码进行炔丙醇型三萜烯的24位的羟基化的酶蛋白，并且还提供了使用所述基因使用的方法 的遗传工程技术。
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公开(公告)号：US20110171698A1
公开(公告)日：2011-07-14
申请号：US12690732
申请日：2010-01-20
申请人： Yutaka Ebizuka , Masaaki Shibuya , Eriko Wakita
发明人： Yutaka Ebizuka , Masaaki Shibuya , Eriko Wakita
CPC分类号： C12P33/06 , C12N9/0077
摘要： β-Amyrin, a precursor in biosynthesis of soyasapogenol B, is biosynthesized by cyclization of 2,3-oxidosqualene which is generated by the mevalonate pathway, and soyasapogenol B is biosynthesized by two hydroxylations of β-amyrin. However, a gene of 22-hydroxylase involved in the sequence of reactions has not been identified. The present inventors identified a gene encoding the hydroxylase for oleanene triterpenes at C-22, and found that oleanene triterpenes could be hydroxylated at C-22 by co-expressing this gene together with one or more specific genes. Further, the present inventors found that soyasapogenol B could be efficiently produced in large quantities by co-expressing this gene for 22-hydroxylase with a gene for 24-hydroxylase.
摘要翻译： 大豆皂甙B的生物合成中的前体-Amyrin是通过由甲羟戊酸途径产生的2,3-氧化山梨醇环化而生物合成的，而大豆皂甙元B通过二元羟基化生物合成。 然而，涉及反应顺序的22-羟化酶的基因尚未确定。 本发明人鉴定了C-22中编码烯烃三萜类羟基化酶的基因，发现通过与一个或多个特定基因共表达该基因，可以在C-22上将含烯烃三萜类化合物羟基化。 此外，本发明人发现通过用24-羟化酶的基因共表达22-羟化酶的基因可以大量高效地生产大豆皂草酚B。
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