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    Muteins of .beta.-galactosidase fragments having increased activity
    2.
    发明授权
    Muteins of .beta.-galactosidase fragments having increased activity 失效
    β-半乳糖苷酶片段的突变蛋白具有增加的活性

    公开(公告)号：US5492813A

    公开(公告)日：1996-02-20

    申请号：US146673

    申请日：1993-11-01

    申请人： Scott J. Eisenbeis ,  Sophie J. Boguslawski ,  Mark Krevolin ,  David J. Ledden

    发明人： Scott J. Eisenbeis ,  Sophie J. Boguslawski ,  Mark Krevolin ,  David J. Ledden

    IPC分类号： C12N9/38 ,  C12N15/09 ,  C12R1/19 ,  G01N33/535 ,  G01N33/542 ,  C12N15/56

    CPC分类号： C12Y302/01023 ,  C12N9/2471 ,  G01N33/535 ,  Y10S435/963

    摘要： Muteins of enzyme acceptor polypeptide fragments of .beta.-galactosidase are provided which exhibit substantially increased kinetic complementation activity with no significant loss in stability. A preferred enzyme acceptor fragment has an amino acid other than cysteine located at position 500 of the natural sequence. An especially preferred substitution is serine or valine. Other preferred muteins have an amino acid other than methionine located at position 443, with leucine being especially preferred, or an amino acid other than cysteine at position 76, with leucine being an especially preferred substitution. Also provided are methods for producing the novel muteins, reagent compositions comprising the novel muteins, and immunoassay methods for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active .beta.-galactosidase.

    摘要翻译： 提供了β-半乳糖苷酶的酶受体多肽片段的突变蛋白，其表现出显着增加的动力学互补活性，而没有显着的稳定性损失。 优选的酶受体片段具有位于天然序列的位置500处的半胱氨酸以外的氨基酸。 特别优选的取代是丝氨酸或缬氨酸。 其他优选的突变蛋白具有除位于第443位的甲硫氨酸以外的氨基酸，特别优选亮氨酸，或位置76处的半胱氨酸以外的氨基酸，亮氨酸是特别优选的取代基。 还提供了用于产生新型突变蛋白的方法，包含新颖突变蛋白的试剂组合物和用于测定其中新型突变蛋白与酶供体多肽片段重组以形成酶活性β-半乳糖苷酶的分析物的免疫测定方法。

    Native analyte as a reference in lateral flow assays
    4.
    发明授权
    Native analyte as a reference in lateral flow assays 有权
    天然分析物作为侧流测定中的参考

    公开(公告)号：US08101429B2

    公开(公告)日：2012-01-24

    申请号：US10557300

    申请日：2004-06-03

    申请人： Gary H. Krauth ,  David J. Ledden

    发明人： Gary H. Krauth ,  David J. Ledden

    IPC分类号： G01N33/53

    CPC分类号： G01N33/558 ,  G01N33/56911 ,  G01N33/56927 ,  G01N33/56988 ,  G01N33/571 ,  G01N33/6854 ,  G01N33/6887 ,  G01N33/689 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/81

    摘要： This invention is directed to a lateral flow assay for detecting the presence of an analyte in a liquid test sample. The lateral flow assay represents an improvement in the ability to accurately and with high fidelity to detect the presence or absence of a target analyte in a liquid sample, in part, by encompassing a reference region of immobilized, non-diffusible analyte that allows for detection of any factors that interfere with the interaction and binding of the analyte to the labeled capture reagent. Any influences on the interaction and binding of the analyte that is free in solution in the liquid test sample to its complementary labeled reagent will be encountered in parallel in the binding between the immobilized analyte in reference region to the labeled reagent as it diffuses through the reference region. In one embodiment, the lateral flow assay of the invention is a urine-based human Chorionic Gonadotropin (hCG) assay.

    摘要翻译： 本发明涉及用于检测液体测试样品中分析物的存在的侧向流动测定。 横向流量测定表示准确地并且以高保真度检测液体样品中目标分析物的存在或不存在的能力的改进，部分地通过包含允许检测的固定的不扩散分析物的参考区域 干扰分析物与标记的捕获试剂的相互作用和结合的任何因素。 在液体测试样品中溶液中游离的分析物与其互补的标记试剂之间的相互作用和结合的任何影响将在参考区域中固定化分析物与标记试剂之间的结合中并行遇到，因为它通过参考文献扩散 地区。 在一个实施方案中，本发明的侧流测定是基于尿液的人绒毛膜促性腺激素（hCG）测定。

    Automatic identification of reagent test strips using reflectance values
    5.
    发明授权
    Automatic identification of reagent test strips using reflectance values 有权
    使用反射率值自动识别试剂试纸

    公开(公告)号：US07538336B2

    公开(公告)日：2009-05-26

    申请号：US10556097

    申请日：2004-06-03

    申请人： David A. Brock ,  Chris T. Zimmerle ,  David J. Ledden

    发明人： David A. Brock ,  Chris T. Zimmerle ,  David J. Ledden

    IPC分类号： G01N21/86

    CPC分类号： G01N21/251 ,  G01N21/64 ,  G01N21/8483

    摘要： Identifying a test product having a test region includes impinging on the test region a set of test signals at known test wavelengths. An image of the test region is generated from the reflected signals. The image is comprised of a set of reflectance values that are compared against reference reflectance values at the test signal wavelengths to determine the test product type.

    摘要翻译： 识别具有测试区域的测试产品包括在测试区域上照射已知测试波长的一组测试信号。 从反射信号产生测试区域的图像。 该图像由与测试信号波长的参考反射率值进行比较以反映测试产品类型的一组反射率值组成。

    Readhead for optical inspection apparatus
    6.
    发明授权
    Readhead for optical inspection apparatus 有权
    光学检测仪器的读数头

    公开(公告)号：US07499154B2

    公开(公告)日：2009-03-03

    申请号：US10556299

    申请日：2004-06-03

    申请人： Nicholas T. Stock ,  Gary D. Ross ,  David J. Ledden

    发明人： Nicholas T. Stock ,  Gary D. Ross ,  David J. Ledden

    IPC分类号： G01N21/00

    CPC分类号： G01N21/251 ,  G01N21/64 ,  G01N21/8483

    摘要： A readhead for illuminating a sample carrier and receiving light from the sample carrier, including a housing for receiving a sample carrier, an array of light sources mounted within the housing in a fixed position relative to the sample carrier, and including first and second light-emitting diodes for emitting substantially monochromatic light of a two different wavelengths, a light guide mounted in the housing between the light-emitting diodes and the sample carrier, and a light detector coupled to receive light from the sample carrier. The readhead also includes a light source for directing excitation light of a predetermined wavelength to the sample carrier, and a light filter positioned between the sample carrier and the light detector and adapted to prevent passage therethrough of the excitation light. The readhead allows both fluorescence spectroscopy and reflectance spectroscopy to be conducted on the sample carrier.

    摘要翻译： 一种用于照亮样品载体并从样品载体接收光的读取头，包括用于接收样品载体的壳体，安装在壳体内相对于样品载体固定位置的光源阵列，并且包括第一和第二发光体， 用于发射两个不同波长的基本单色光的发光二极管，安装在发光二极管和样品载体之间的壳体中的光导，以及耦合以接收来自样品载体的光的光检测器。 读取头还包括用于将预定波长的激发光引导到样品载体的光源，以及位于样品载体和光检测器之间并适于防止激发光通过的滤光器。 读数头允许在样品载体上进行荧光光谱和反射光谱。

    Specific binding assays employing label analog to reduce sample interferences
    8.
    发明授权
    Specific binding assays employing label analog to reduce sample interferences 失效
    使用标记类似物进行特异性结合测定以减少样本干扰

    公开(公告)号：US4810635A

    公开(公告)日：1989-03-07

    申请号：US852418

    申请日：1986-04-16

    申请人： David J. Ledden ,  Dwight E. Schroedter

    发明人： David J. Ledden ,  Dwight E. Schroedter

    IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N33/58 ,  G01N33/94 ,  C07H17/00 ,  C07H19/00 ,  C07H21/00

    CPC分类号： G01N33/542 ,  G01N33/5306 ,  G01N33/581 ,  G01N33/9453 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S436/825

    摘要： A specific binding assay, e.g., immunoassay, method and reagent system wherein a structural analog of the label portion of the labeled reagent is included in the reaction mixture to interact with potentially interfering substances in the test sample. The label analog is selected or designed to be substantially inactive in the assay detection system. The invention improves the precision of the assays, particularly homogeneous immunoassays, by significantly reducing or eliminating the variable interaction, e.g., binding, of the labeled reagent by interfering substances in the test sample.

    摘要翻译： 特异性结合测定，例如免疫测定，方法和试剂系统，其中标记试剂的标记部分的结构类似物包含在反应混合物中以与测试样品中的潜在干扰物质相互作用。 在测定检测系统中选择或设计标记类似物基本上是无活性的。 本发明通过显着减少或消除由测试样品中的干扰物质引起的标记试剂的可变相互作用（例如结合），提高了测定的精度，特别是均一的免疫测定。