公开(公告)号：US20050053943A1

公开(公告)日：2005-03-10

申请号：US10608388

申请日：2003-06-30

申请人： Seishi Kato ,  Shingo Sekine ,  Tomoko Yamaguchi

发明人： Seishi Kato ,  Shingo Sekine ,  Tomoko Yamaguchi

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K48/00 ,  A61P35/00 ,  A61P43/00 ,  C07K14/47 ,  C07K14/705 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12R1/19 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/74

CPC分类号： C07K14/705 ,  A61K38/00

摘要： A human membrane antigen TM4 superfamily protein existing on the osteosarcoma cell surface and a cDNA encoding this protein is provided, said protein being useful as a pharmaceutical for treatment and diagnosis of cancers and also as an antigen for preparation of an antibody against said protein.

摘要翻译： 提供存在于骨肉瘤细胞表面上的人膜抗原TM4超家族蛋白质和编码该蛋白质的cDNA，所述蛋白质可用作癌症治疗和诊断的药物，也可用作制备针对所述蛋白质的抗体的抗原。

公开(公告)号：US06280968B1

公开(公告)日：2001-08-28

申请号：US09065019

申请日：1998-04-17

申请人： Seishi Kato ,  Tomoko Yamaguchi ,  Shingo Sekine ,  Kouju Kamata

发明人： Seishi Kato ,  Tomoko Yamaguchi ,  Shingo Sekine ,  Kouju Kamata

IPC分类号： C12P2106

CPC分类号： C07K14/575 ,  A61K38/00 ,  C07K2319/00

摘要： The present invention provides a human PEC-60-like protein, a gastrointestinal hormone excreted by a stomach tissue and a cDNA encoding this protein. The protein and the gene of the present invention can provide a protein containing the amino acid sequence represented by Sequence No. 1 and a DNA encoding said protein exemplified as a cDNA containing the base sequence represented by Sequence No. 1. as well as a human cDNA encoding a human PEC-60-like protein and said protein by the expression of this human cDNA recombinant.

摘要翻译： 本发明提供人类PEC-60样蛋白质，由胃组织排出的胃肠激素和编码该蛋白质的cDNA。 本发明的蛋白质和基因可以提供含有序列号1所示的氨基酸序列的蛋白质和编码所述蛋白质的DNA，该DNA作为含有序列号1表示的碱基序列的cDNA，以及人 通过该人cDNA重组体的表达，编码人类PEC-60样蛋白质的cDNA和所述蛋白质。

公开(公告)号：US06703201B1

公开(公告)日：2004-03-09

申请号：US09671483

申请日：2000-09-27

申请人： Seishi Kato ,  Tomoko Yamaguchi ,  Shingo Sekine ,  Kouju Kamata

发明人： Seishi Kato ,  Tomoko Yamaguchi ,  Shingo Sekine ,  Kouju Kamata

IPC分类号： C12Q168

CPC分类号： C07K14/575 ,  A61K38/00

摘要： The present invention provides a human PEC-60-like protein, a gastrointestinal hormone excreted by a stomach tissue and a cDNA encoding this protein. The protein and the gene of the present invention can provide a protein containing the amino acid sequence represented by Sequence No. 1 and a DNA encoding said protein exemplified as a cDNA containing the base sequence represented by Sequence No. 1 as well as a human cDNA encoding a human PEC-60-like protein and said protein by the expression of this human cDNA recombinant.

摘要翻译： 本发明提供人类PEC-60样蛋白质，由胃组织排出的胃肠激素和编码该蛋白质的cDNA。 本发明的蛋白质和基因可以提供含有序列号1所示的氨基酸序列的蛋白质和编码所述蛋白质的DNA，作为含有序列号1表示的碱基序列的cDNA，以及人cDNA 通过该人cDNA重组体的表达编码人类PEC-60样蛋白和所述蛋白质。

公开(公告)号：US5527897A

公开(公告)日：1996-06-18

申请号：US151391

申请日：1993-11-12

申请人： Kinichiro Oda ,  Susumu Nakada ,  Eiji Hara ,  Tomoko Yamaguchi ,  Takeshi Nakamura ,  Yumiko Oka ,  Toshihiko Kishimoto

发明人： Kinichiro Oda ,  Susumu Nakada ,  Eiji Hara ,  Tomoko Yamaguchi ,  Takeshi Nakamura ,  Yumiko Oka ,  Toshihiko Kishimoto

IPC分类号： A61K31/70 ,  A61K38/00 ,  C07H21/04 ,  C07K14/00 ,  C07K14/47 ,  C12N1/21 ,  C12N15/09 ,  C12N15/12 ,  C12Q1/68 ,  C12R1/19 ,  C12N15/00

CPC分类号： C07K14/4703 ,  A61K38/00

摘要： Disclosed herein are Human Id genes (Id-1H and Id-1H') and variants thereof. Origin, preparation process, biochemical functions and the like: two synthetic oligonucleotides separately having base sequences corresponding to regions of amino acid sequence, which are almost completely conserved by three mouse Id genes (Id-1, Id-2 and HLH462) are used as probes to screen clones having respective cDNAs complementary to the probes from a cDNA library of human TIG-1 fibroblasts in accordance with the hybridization method, thereby isolating human Id genes. The expression of the human Id genes specifically varies according to the state of cell proliferation, in particular, the acquisition of cell aging and permanently proliferating ability.Expression vectors with the human Id gene or variant thereof integrated therein, transformed cell strains with the expression vector introduced therein, diagnostic probes obtained by chemically synthesizing a partial sequence of the base sequence of the human Id gene, or a variation sequence thereof, and antisense oligonucleotides composed of a base sequences complementary to the partial sequence of the base sequence of the human Id gene, or the variation sequence thereof are also disclosed.

摘要翻译： 本文公开了人Id基因（Id-1H和Id-1H'）及其变体。 原产地，制备方法，生物化学功能等：分别具有对应于三个小鼠Id基因（Id-1，Id-2和HLH462）几乎完全保守的氨基酸序列区域的碱基序列的两个合成寡核苷酸被用作 探针根据杂交方法筛选具有与人TIG-1成纤维细胞的cDNA文库互补的各cDNA的克隆，从而分离人Id基因。 人Id基因的表达特异性地根据细胞增殖的状态而变化，特别是获得细胞衰老和永久增殖能力。 其中整合了人Id基因或其变体的表达载体，其中引入了表达载体的转化细胞株，通过化学合成人Id基因的碱基序列的部分序列或其变异序列获得的诊断探针，以及反义 还公开了由与人Id基因的碱基序列的部分序列互补的碱基序列或其变异序列组成的寡核苷酸。

公开(公告)号：US06566141B2

公开(公告)日：2003-05-20

申请号：US09827908

申请日：2001-04-09

申请人： Hideshi Fujiwake ,  Tomoko Yamaguchi

发明人： Hideshi Fujiwake ,  Tomoko Yamaguchi

IPC分类号： G01N33483

CPC分类号： C12Q1/6827 ,  Y10T436/143333 ,  C12Q2537/113 ,  C12Q2527/107

摘要： A solution containing double stranded nucleic acid is heated, thereby dissociating the strands, which in turn are cooled and rebounded. A homoduplex and a heteroduplex are formed when the double stranded nucleic acid is a hetero body, while only a homoduplex is formed when the double stranded nucleic acid is a homo body. The temperature of the solution is increased while measuring ultraviolet absorption, thereby acquiring a thermal melting profile. When the heated and cooled solution contains only a homoduplex, the thermal melting profile has a single Tm temperature or two Tm temperatures close to each other. When the heated and cooled solution contains a homoduplex and a heteroduplex, the thermal melting profile has two separate Tm temperatures or further single Tm temperatures close to each other. The method therefore allows for the detection of a heteroduplex or a mutation in a sample double stranded nucleic acid.

摘要翻译： 加热含有双链核酸的溶液，从而解离链，其又被冷却和回弹。 当双链核酸是杂体时，形成同源双链和异源二聚体，而当双链核酸是同体时，仅形成同源双链体。 在测量紫外线吸收的同时，溶液的温度升高，从而获得热熔融曲线。 当加热和冷却的溶液仅含有同型双链体时，热熔融分布具有单个Tm温度或彼此接近的两个Tm温度。 当加热和冷却的溶液含有均质双链和异源双链时，热熔融分布具有彼此接近的两个单独的Tm温度或更高的单个Tm温度。 因此，该方法允许检测样品双链核酸中的异源双链体或突变。

公开(公告)号：US07020664B1

公开(公告)日：2006-03-28

申请号：US09723972

申请日：2000-11-28

申请人： Tomoko Yamaguchi ,  Toshio Mizuno ,  Motoshi Suzuki

发明人： Tomoko Yamaguchi ,  Toshio Mizuno ,  Motoshi Suzuki

IPC分类号： G06F12/00

CPC分类号： G06F17/30067

摘要： Upon receipt of a segment name obtainment request, the segment name generation unit 104 generates a segment name composed of (1) a file name of a file to which the segment belongs and (2) a number indicating a serial number of the segment in the file. Upon receipt of an access request specifying a segment name, the segment identification unit 119 refers to the segment name to detect the file name and the serial number of the segment in the detected file. The segment position detection unit 106 then refers to the file information storage unit 102 and the segment information storage unit 103 to detect a position where a segment is to be accessed.

摘要翻译： 在接收到段名称获取请求时，段名称生成单元104生成由（1）段所属的文件的文件名构成的段名，（2）表示段的名称的序号， 文件。 在接收到指定段名称的访问请求时，段识别单元119参考段名来检测检测文件中的段的文件名和序列号。 然后，段位置检测单元106参考文件信息存储单元102和段信息存储单元103以检测段被访问的位置。