Human PEC-60-like protein and DNA encoding the same
    1.
    发明授权
    Human PEC-60-like protein and DNA encoding the same 失效
    人类PEC-60样蛋白和编码相同的DNA

    公开(公告)号:US06280968B1

    公开(公告)日:2001-08-28

    申请号:US09065019

    申请日:1998-04-17

    IPC分类号: C12P2106

    摘要: The present invention provides a human PEC-60-like protein, a gastrointestinal hormone excreted by a stomach tissue and a cDNA encoding this protein. The protein and the gene of the present invention can provide a protein containing the amino acid sequence represented by Sequence No. 1 and a DNA encoding said protein exemplified as a cDNA containing the base sequence represented by Sequence No. 1. as well as a human cDNA encoding a human PEC-60-like protein and said protein by the expression of this human cDNA recombinant.

    摘要翻译: 本发明提供人类PEC-60样蛋白质,由胃组织排出的胃肠激素和编码该蛋白质的cDNA。 本发明的蛋白质和基因可以提供含有序列号1所示的氨基酸序列的蛋白质和编码所述蛋白质的DNA,该DNA作为含有序列号1表示的碱基序列的cDNA,以及人 通过该人cDNA重组体的表达,编码人类PEC-60样蛋白质的cDNA和所述蛋白质。

    Human PEC-60-like protein and DNA encoding this protein
    2.
    发明授权
    Human PEC-60-like protein and DNA encoding this protein 有权
    人类PEC-60样蛋白和编码该蛋白的DNA

    公开(公告)号:US06703201B1

    公开(公告)日:2004-03-09

    申请号:US09671483

    申请日:2000-09-27

    IPC分类号: C12Q168

    CPC分类号: C07K14/575 A61K38/00

    摘要: The present invention provides a human PEC-60-like protein, a gastrointestinal hormone excreted by a stomach tissue and a cDNA encoding this protein. The protein and the gene of the present invention can provide a protein containing the amino acid sequence represented by Sequence No. 1 and a DNA encoding said protein exemplified as a cDNA containing the base sequence represented by Sequence No. 1 as well as a human cDNA encoding a human PEC-60-like protein and said protein by the expression of this human cDNA recombinant.

    摘要翻译: 本发明提供人类PEC-60样蛋白质,由胃组织排出的胃肠激素和编码该蛋白质的cDNA。 本发明的蛋白质和基因可以提供含有序列号1所示的氨基酸序列的蛋白质和编码所述蛋白质的DNA,作为含有序列号1表示的碱基序列的cDNA,以及人cDNA 通过该人cDNA重组体的表达编码人类PEC-60样蛋白和所述蛋白质。

    cDNAs coding for human proteins having transmembrane domains
    4.
    发明授权
    cDNAs coding for human proteins having transmembrane domains 失效
    编码具有跨膜结构域的人蛋白质的cDNA

    公开(公告)号:US06500939B1

    公开(公告)日:2002-12-31

    申请号:US09529157

    申请日:2000-08-21

    IPC分类号: C07H2102

    CPC分类号: C07K14/705

    摘要: The invention provides cDNAs coding for human proteins having transmembrane domains and eucaryotic cells expressing said cDNAs. The cDNAs of the invention can be utilized as probes for the gene diagnosis and gene sources for the gene therapy. Furthermore, the cDNAs can be utilized for large-scale expression of said proteins. Cells, wherein these membrane protein genes are introduced and membrane proteins are expressed in large amounts, can be utilized for detection of the corresponding ligands, screening of novel low-molecular pharmaceuticals, and so on.

    摘要翻译: 本发明提供了编码具有跨膜结构域的人蛋白质和表达所述cDNA的真核细胞的cDNA。 本发明的cDNA可用作基因诊断的探针和基因治疗的基因来源。 此外,cDNA可用于大规模表达所述蛋白质。 其中引入这些膜蛋白基因和大量表达膜蛋白的细胞可用于检测相应的配体,筛选新的低分子药物等。

    Process for producing cDNAs with complete length, process for producing
intermediates thereof and process for producing vectors containing
cDNAs with complete lengths
    6.
    发明授权
    Process for producing cDNAs with complete length, process for producing intermediates thereof and process for producing vectors containing cDNAs with complete lengths 失效
    用于生产具有完整长度的cDNA的方法,其制备中间体的方法和用于生产含有完整长度的cDNA的载体的方法

    公开(公告)号:US5597713A

    公开(公告)日:1997-01-28

    申请号:US244188

    申请日:1994-07-25

    CPC分类号: C12N15/1096 C12N15/66

    摘要: A process for providing cDNAs containing full primary structure information of proteins by selectively synthesizing full-length cDNAs containing sequences starting from the capped region of mRNAs is disclosed. The invention provides a process for producing an intermediate for the synthesis of a full-length cDNA, which comprises the steps of treating mRNA extracted from cells to eliminate the phosphate group from the 5' end of an uncapped degraded mRNA; decapping from the 5' end of a capped intact mRNA; and ligating either a DNA oligonucleotide or a DNA-RNA chimeric oligonucleotide represented by the following general formula [I] to the 5' end phosphate group formed in the above step by the action of T4 RNA ligase, thereby selectively adding either the DNA oligonucleotide or the DNA-RNA chimeric oligonucleotide having an arbitrary sequence to the 5' end of the intact mRNA.5'-dN1-dN2- . . . -dNm-N1-N2- . . . -Nn-3' [I]The present invention also provides a process for synthesizing a full-length cDNA, which comprises linking a double-stranded DNA primer having a dT tail by annealing to a poly(A) tail of the 3' end of the intact mRNA having the DNA oligonucleotide or the DNA-RNA chimeric nucleotide added at the 5' end produced by the above-mentioned process, and then synthesizing the first strand cDNA complementary to the intact mRNA by a reverse transcriptase.

    摘要翻译: PCT No.PCT / JP93 / 01359 Sec。 371日期:1994年7月25日 102(e)日期1994年7月25日PCT提交1993年9月22日PCT公布。 第WO94 / 08001号公报 日期1994年04月14日公开了通过选择性地合成含有从mRNA封端区开始的序列的全长cDNA提供含有蛋白质全部一级结构信息的cDNA的方法。 本发明提供了一种制备用于合成全长cDNA的中间体的方法,其包括以下步骤:处理从细胞中提取的mRNA,以从未封端的降解的mRNA的5'末端消除磷酸基团; 从覆盖的完整mRNA的5'端开始切割; 并通过T4 RNA连接酶的作用将由以下通式[I]表示的DNA寡核苷酸或DNA-RNA嵌合寡核苷酸与上述步骤中形成的5'端磷酸基结合,从而选择性地添加DNA寡核苷酸或 具有与完整mRNA的5'末端任意序列的DNA-RNA嵌合寡核苷酸。 5'-dN1-dN2-。 。 。 -dNm-N1-N2-。 。 。 -Nn-3'[I]本发明还提供了一种合成全长cDNA的方法,其包括将具有dT尾的双链DNA引物与3'端的poly(A)末端退火连接 的具有通过上述方法制备的5'端加入的DNA寡核苷酸或DNA-RNA嵌合核苷酸的完整mRNA,然后通过逆转录酶合成与完整mRNA互补的第一链cDNA。

    Method of synthesizing cDNA
    9.
    发明授权
    Method of synthesizing cDNA 有权
    cDNA合成方法

    公开(公告)号:US08067205B2

    公开(公告)日:2011-11-29

    申请号:US10550788

    申请日:2004-03-29

    IPC分类号: C12P19/34

    CPC分类号: C12N15/1096

    摘要: A method for synthesizing cDNA possessing a consecutive sequence starting with a nucleotide adjacent to a cap structure of mRNA, which comprises (i) a process for annealing a double-stranded DNA primer and an RNA mixture containing mRNA possessing a cap structure, (ii) a process for preparing a conjugate of an mRNA/cDNA heteroduplex and a double-stranded DNA primer by synthesizing the first-strand cDNA primed with the double-stranded DNA primer using reverse transcriptase, and (iii) a process for circularizing the conjugate of the mRNA/cDNA heteroduplex and the double-stranded DNA primer by joining the 3′ and 5′ ends of the DNA strand containing cDNA using ligase. This method enables us to efficiently synthesize a full-length cDNA possessing a consecutive sequence starting with a transcription-start-site nucleotide from a small amount of RNA by small processes.

    摘要翻译: 一种合成cDNA的方法,该方法具有从与mRNA结构相邻的核苷酸开始的连续序列,其包括(i)退火双链DNA引物的方法和含有具有帽结构的mRNA的RNA混合物,(ii) 通过使用逆转录酶合成用双链DNA引物引发的第一链cDNA来制备mRNA / cDNA异源双链和双链DNA引物的缀合物的方法,和(iii)使 通过使用连接酶连接含有cDNA的DNA链的3'和5'末端,mRNA / cDNA异源双链和双链DNA引物。 该方法使得我们能够通过小过程有效地合成具有从少量RNA转录起始位点核苷酸开始的连续序列的全长cDNA。

    Human nuclear protein having WW domain and polynucleotide encoding the same
    10.
    发明授权
    Human nuclear protein having WW domain and polynucleotide encoding the same 失效
    具有WW结构域的人核蛋白和编码其的多核苷酸

    公开(公告)号:US07067642B1

    公开(公告)日:2006-06-27

    申请号:US09889722

    申请日:2000-11-22

    摘要: An isolated and purified human nucleoprotein containing the amino acid sequence of SEQ ID NO:1; a polynucleotide encoding this protein; and an antibody against this protein. The above protein and antibody are useful in diagnosing and treating the pathogenic conditions of cancer, etc. The above polynucleotide is usable in acquiring the protein in a large amount. By screening a low-molecular weight compound binding to this protein, drugs of a novel type (antitumor agents, etc.) can be searched for.

    摘要翻译: 包含SEQ ID NO:1的氨基酸序列的分离和纯化的人核蛋白; 编码该蛋白质的多核苷酸; 和针对该蛋白质的抗体。 上述蛋白质和抗体可用于诊断和治疗癌症等病原性病症。上述多核苷酸可用于大量获取蛋白质。 通过筛选与该蛋白质结合的低分子量化合物,可以搜索新型的药物(抗肿瘤剂等)。