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113 条记录 (耗时 0.071 秒)

    Naturally occurring gangliosides containing de-N-acetyl-sialic acid and their applications as modifiers of cell physiology
    1.
    发明授权
    Naturally occurring gangliosides containing de-N-acetyl-sialic acid and their applications as modifiers of cell physiology 失效
    含有脱-N-乙酰唾液酸的天然存在的神经节苷脂及其作为细胞生理学改性剂的应用

    公开(公告)号：US5272138A

    公开(公告)日：1993-12-21

    申请号：US467458

    申请日：1990-01-19

    申请人： Sen-itiroh Hakomori ,  Gustavo A. Nores ,  Nobuo Hanai ,  Taeko Dohi ,  Steven B. Levery ,  Mary Ellen K. Salyan ,  Hisao Nojiri

    发明人： Sen-itiroh Hakomori ,  Gustavo A. Nores ,  Nobuo Hanai ,  Taeko Dohi ,  Steven B. Levery ,  Mary Ellen K. Salyan ,  Hisao Nojiri

    IPC分类号： A61K31/70 ,  C07H15/10 ,  A61K31/725 ,  C07H5/06 ,  C07H13/06

    CPC分类号： C07H15/10 ,  A61K31/70

    摘要： A method for preparing de-N-acylated forms of an N-acyl sugar-containing glycosphingolipid and lyso forms of glycosphingolipid comprising hydrolyzing the glycosphingolipids under mild alkaline conditions such that the N-acyl group of the sugar moiety is preferentially hydrolyzed. Substantially pure gangliosides containing de-N-acetyl-sialic acid isolated from natural sources. A culture medium for stimulating growth of human and animal cells comprising: essential nutrients for cell growth, and a cell growth stimulatory amount of one or more gangliosides containing de-N-acetyl-sialic acid. A method for stimulating growth of human and animal cells cultured in vitro with a cell growth stimulatory amount of one or more gangliosides containing de-N-acetyl-sialic acid. A medicament and method for stimulating or inhibiting growth of human and animal cells by stimulating or inhibiting the production of growth stimulators selected from the group consisting of gangliosides containing de-N-acetyl-sialic acid, substances that promote synthesis of gangliosides containing de-N-acetyl-sialic acid and pharmaceutically acceptable salts thereof.

    摘要翻译： 一种制备N-酰基糖型鞘糖脂的N-脱酰基形式的方法，其包括在温和碱性条件下水解糖鞘脂，使糖部分的N-酰基优先水解。 含有从天然来源分离的脱-N-乙酰唾液酸的纯粹神经节苷脂。 用于刺激人和动物细胞生长的培养基包括：用于细胞生长的必需营养物和含有脱-N-乙酰唾液酸的一种或多种神经节苷脂的细胞生长刺激量。 用于刺激体外培养的细胞生长刺激量的含有脱-N-乙酰唾液酸的一种或多种神经节苷脂的人和动物细胞生长的方法。 通过刺激或抑制选自包含脱-N-乙酰唾液酸的神经节苷脂的生长刺激剂的生成来刺激或抑制人和动物细胞生长的药物和方法，促进含有脱-N-乙酰唾液酸的神经节苷脂合成的物质 乙酰唾液酸及其药学上可接受的盐。

    ALPHA-1,3-FUCOSYLTRANSFERASE
    7.
    发明申请
    ALPHA-1,3-FUCOSYLTRANSFERASE 失效

    公开(公告)号：US20060246468A1

    公开(公告)日：2006-11-02

    申请号：US11276029

    申请日：2006-02-10

    申请人： Katsutoshi Sasaki ,  Kazumi Miura ,  Nobuo Hanai ,  Tatsunari Nishi

    发明人： Katsutoshi Sasaki ,  Kazumi Miura ,  Nobuo Hanai ,  Tatsunari Nishi

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C08B37/00 ,  C12P21/06 ,  C12N9/10 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/1051

    摘要： The invention provides a novel species of α-1,3-fucosyltransferase expressed by a gene cloned from animal cells, a cDNA coding for the α-1,3-fucosyltransferase, a method of detecting, or inhibiting the production of, the α-1,3-fucosyltransferase which involves the use of the cDNA, a recombinant vector containing the DNA as an insert, a cell harboring the recombinant vector, and a method of producing same. The α-1,3-fucosyltransferase of the invention is useful in the production of carbohydrate chains having useful physiological activity, for example sialyl Lewis x, and modifications thereof.

    摘要翻译： 本发明提供由从动物细胞克隆的基因表达的α-1,3-岩藻糖基转移酶的新型，编码α-1,3-岩藻糖基转移酶的cDNA，检测或抑制α-1,3-岩藻糖基转移酶的产生的方法， 涉及使用cDNA的1,3-岩藻糖基转移酶，含有DNA作为插入片段的重组载体，携带重组载体的细胞及其生产方法。 本发明的α-1,3-岩藻糖基转移酶可用于生产具有有用生理活性的碳水化合物链，例如唾液酸基路易斯x及其修饰。