公开(公告)号：US20230235390A1

公开(公告)日：2023-07-27

申请号：US18156739

申请日：2023-01-19

Applicant: Shanghai Blood Center

Inventor： Luyi YE ,  Huijun ZHU ,  Ruishu LI ,  Min FU ,  Ping LU ,  Ziyan ZHU

IPC: C12Q1/6872 ,  C12Q1/6806 ,  C12Q1/6853

CPC classification number: C12Q1/6872 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention provides a mass spectrometry-based method and a kit for genotyping of platelet and neutrophil antigens and glycoproteins, which are used for genotyping of platelet-specific antigens, platelet CD36 glycoproteins and neutrophil antigens; by designing an optimal primer combination, problems such as homologous sequences and rich GC are overcome, moreover, by improving amplification reaction conditions and using nucleic acid mass spectrometry as a platform, 35 platelet-specific antigen polymorphic sites, 10 CD36 polymorphic sites and 8 neutrophil antigen polymorphic sites can be simultaneously detected in 2 reactions. The present invention has the characteristics of high specificity and sensitivity, and fast and high throughput, and can be used in clinic, scientific research, platelet donor routine screening, etc.