公开(公告)号：US20230235396A1

公开(公告)日：2023-07-27

申请号：US18156775

申请日：2023-01-19

Applicant: Shanghai Blood Center

Inventor： Luyi YE ,  Qixiu YANG ,  Ziyan ZHU

IPC: C12Q1/6876

CPC classification number: C12Q1/6876 ,  C12Q2600/156

Abstract: The present invention provides a mass spectrometry-based method and kit for erythrocyte blood group genotyping. By taking nucleic acid mass spectrometry as a platform and by designing primer combinations and improving amplification reaction conditions, 61 blood group genetic sites in 21 erythrocyte blood group systems can be simultaneously detected in one reaction, rapid typing of the 21 erythrocyte blood group systems can be realized, and identified phenotypes are all clinically significant erythrocyte antigen phenotypes. The present invention has the characteristics of high sensitivity, strong specificity, simple operation, and rapid and high throughput. The present invention can be applied to difficult blood group identification, blood matching, rare blood group screening, scientific research, routine business development and the like in clinical practice. A

公开(公告)号：US20230235390A1

公开(公告)日：2023-07-27

申请号：US18156739

申请日：2023-01-19

Applicant: Shanghai Blood Center

Inventor： Luyi YE ,  Huijun ZHU ,  Ruishu LI ,  Min FU ,  Ping LU ,  Ziyan ZHU

IPC: C12Q1/6872 ,  C12Q1/6806 ,  C12Q1/6853

CPC classification number: C12Q1/6872 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention provides a mass spectrometry-based method and a kit for genotyping of platelet and neutrophil antigens and glycoproteins, which are used for genotyping of platelet-specific antigens, platelet CD36 glycoproteins and neutrophil antigens; by designing an optimal primer combination, problems such as homologous sequences and rich GC are overcome, moreover, by improving amplification reaction conditions and using nucleic acid mass spectrometry as a platform, 35 platelet-specific antigen polymorphic sites, 10 CD36 polymorphic sites and 8 neutrophil antigen polymorphic sites can be simultaneously detected in 2 reactions. The present invention has the characteristics of high specificity and sensitivity, and fast and high throughput, and can be used in clinic, scientific research, platelet donor routine screening, etc.