Engineering enzymatically susceptible proteins
    8.
    发明授权
    Engineering enzymatically susceptible proteins 有权
    工程酶易感蛋白

    公开(公告)号:US08497127B2

    公开(公告)日:2013-07-30

    申请号:US12743418

    申请日:2008-11-21

    IPC分类号: C12N15/00

    CPC分类号: A23K20/189 A23K50/75 C12N9/16

    摘要: The invention provides a synthetic phytase polypeptide which encodes an enzymatically susceptible phytase. Also provided are feed or food products comprising an enzymatically susceptible phytase, and transgenic plants which express the enzymatically susceptible phytase. Further provided are methods for making and using enzymatically susceptible phytases, e.g., a method of using an enzymatically susceptible phytase in feed and food processing.

    摘要翻译: 本发明提供编码酶易感的植酸酶的合成植酸酶多肽。 还提供了包含酶易感的植酸酶的饲料或食品,以及表达酶易感的植酸酶的转基因植物。 进一步提供了制备和使用酶敏感性植酸酶的方法,例如在饲料和食品加工中使用酶促敏感的植酸酶的方法。

    Compositions and Methods for Protein Production
    9.
    发明申请
    Compositions and Methods for Protein Production 有权
    蛋白质生产的组成和方法

    公开(公告)号:US20130084641A1

    公开(公告)日:2013-04-04

    申请号:US13708479

    申请日:2012-12-07

    IPC分类号: C12N15/10

    CPC分类号: C12N15/1089 C12N15/1034

    摘要: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.

    摘要翻译: 本发明提供了选择产生感兴趣多肽的mRNA的方法,包括:a)产生包含编码目的多肽的不同核苷酸序列的单个mRNA序列的阵列; b)确定(a)的每个单独mRNA序列的一个或多个或两个或更多个以下参数:(i)最小自由能(MFE)RNA二级结构; (ii)合奏自由能(EFE); (iii)热力学集合中最小自由能(FMFE)RNA二级结构的频率; 和(iv)集体多样性(ED); c)根据步骤(b)中确定的参数对阵列的各个mRNA序列进行排序; 和d)从步骤(c)的排列阵列中选择mRNA序列,其中所选择的mRNA产生感兴趣的多肽。 本发明还提供了选择用于增强和减少感兴趣的多肽的产生的mRNA的方法。

    METHODS OF DETERMINING PLANT ZYGOSITY USING MASS SPECTROMETRY
    10.
    发明申请
    METHODS OF DETERMINING PLANT ZYGOSITY USING MASS SPECTROMETRY 审中-公开
    使用质谱分析方法确定植物的ZYGOSITY

    公开(公告)号:US20120080590A1

    公开(公告)日:2012-04-05

    申请号:US12897273

    申请日:2010-10-04

    申请人: Shib Sankar Basu

    发明人: Shib Sankar Basu

    IPC分类号: H01J49/00

    摘要: The present invention relates to methods and systems for identifying seeds that are homozygous for an allele of interest and/or for identifying seeds that are likely to produce plants possessing a desired trait. Methods of producing plants that are homozygous for an allele of interest and/or that possess a desired trait are also provided.

    摘要翻译: 本发明涉及用于鉴定对于感兴趣的等位基因是纯合的种子和/或鉴定可能产生具有所需性状的植物的种子的方法和系统。 还提供了产生对目的等位基因是纯合的植物和/或具有所需性状的植物的方法。