公开(公告)号：US08900830B2

公开(公告)日：2014-12-02

申请号：US13812782

申请日：2011-07-28

申请人： Shinji Kita ,  Kazuya Tsuzaki ,  Eitora Yamamura

发明人： Shinji Kita ,  Kazuya Tsuzaki ,  Eitora Yamamura

IPC分类号： C12P13/04 ,  C12P17/18 ,  C12P17/10 ,  C12P7/62 ,  C12N9/16 ,  C12N9/18 ,  C07H21/04 ,  C07K1/00 ,  C07D207/416 ,  C12P41/00 ,  C07D487/20 ,  C12P7/46

CPC分类号： C12P17/10 ,  C07D207/416 ,  C07D487/20 ,  C12N9/18 ,  C12P7/46 ,  C12P41/005

摘要： A process for efficiently producing optically active succinimide derivatives as key intermediates of (3R)-2′-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4′(1′H)-pyrrolo[1,2-a]pyrazine}-1′,2,3′,5(2′H)-tetraone, which comprises the following reaction steps, and the step 2 is performed by using a non-animal-derived enzyme.

摘要翻译： 作为（3R）-2' - （4-溴-2-氟苄基）螺（吡咯烷-3,4'（1'H） - 吡咯并[1,2-a ]吡嗪} -1'，2,3'，5（2'H） - 四酮，其包括以下反应步骤，并且通过使用非动物来源的酶进行步骤2。

公开(公告)号：US20130196388A1

公开(公告)日：2013-08-01

申请号：US13812782

申请日：2011-07-28

申请人： Shinji Kita ,  Kazuya Tsuzaki ,  Eitora Yamamura

发明人： Shinji Kita ,  Kazuya Tsuzaki ,  Eitora Yamamura

IPC分类号： C12P17/10

CPC分类号： C12P17/10 ,  C07D207/416 ,  C07D487/20 ,  C12N9/18 ,  C12P7/46 ,  C12P41/005

摘要： A process for efficiently producing optically active succinimide derivatives as key intermediates of (3R)-2′-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4′(1′H)-pyrrolo[1,2-a]pyrazine}-1′,2,3′,5(2′H)-tetraone, which comprises the following reaction steps, and the step 2 is performed by using a non-animal-derived enzyme.

摘要翻译： 作为（3R）-2' - （4-溴-2-氟苄基）螺（吡咯烷-3,4'（1'H） - 吡咯并[1,2-a ]吡嗪} -1'，2,3'，5（2'H） - 四酮，其包括以下反应步骤，并且通过使用非动物来源的酶进行步骤2。

公开(公告)号：US20090042275A1

公开(公告)日：2009-02-12

申请号：US12208511

申请日：2008-09-11

申请人： Eitora Yamamura ,  Noboru Fujimoto

发明人： Eitora Yamamura ,  Noboru Fujimoto

IPC分类号： C12N1/21 ,  C07H21/00 ,  C12N15/64

CPC分类号： C12P13/008 ,  C12N9/0006 ,  C12N15/70 ,  C12N15/74 ,  C12P13/001

摘要： A shuttle vector is constructed by preparing a DNA region replicable in bacteria belonging to the genus Rhodococcus from a Rhodococcus-derived plasmid having the nucleotide sequence set forth as SEQ ID NO: 73 and a plasmid or its DNA fragment having the nucleotide sequence set forth as SEQ ID NO: 74, and a DNA region replicable in E. coli from an E. coli-derived plasmid or its DNA fragment. An aminoketone asymmetric reductase gene is inserted into the shuttle vector, transformants containing the vector are created, and the aminoketone asymmetric reductase and optically active aminoalcohols are produced.

摘要翻译： 构建穿梭载体，其通过制备可从具有SEQ ID NO：73所示核苷酸序列的红球菌衍生的质粒中的属于红球菌属的细菌中可复制的DNA区域，并且将具有核苷酸序列的质粒或其DNA片段列为 SEQ ID NO：74，以及可从大肠杆菌衍生的质粒或其DNA片段在大肠杆菌中可复制的DNA区域。 将氨基酮不对称还原酶基因插入穿梭载体中，产生含有载体的转化体，产生氨基酮不对称还原酶和光学活性氨基醇。

公开(公告)号：US07446187B2

公开(公告)日：2008-11-04

申请号：US10577296

申请日：2004-10-29

申请人： Eitora Yamamura ,  Noboru Fujimoto

发明人： Eitora Yamamura ,  Noboru Fujimoto

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/63 ,  C12P13/00

CPC分类号： C12P13/008 ,  C12N9/0006 ,  C12N15/70 ,  C12N15/74 ,  C12P13/001

摘要： A shuttle vector is constructed by preparing a DNA region replicable in bacteria belonging to the genus Rhodococcus from a Rhodococcus-derived plasmid having the nucleotide sequence set forth as SEQ ID NO: 73 and a plasmid or its DNA fragment having the nucleotide sequence set forth as SEQ ID NO: 74, and a DNA region replicable in E. coli from an E. coli-derived plasmid or its DNA fragment. An aminoketone asymmetric reductase gene is inserted into the shuttle vector, transformants containing the vector are created, and the aminoketone asymmetric reductase and optically active aminoalcohols are produced.

摘要翻译： 构建穿梭载体，其通过制备可从具有SEQ ID NO：73所示核苷酸序列的红球菌衍生的质粒中的属于红球菌属的细菌中可复制的DNA区域，并且将具有核苷酸序列的质粒或其DNA片段列为 SEQ ID NO：74，以及可从大肠杆菌衍生的质粒或其DNA片段在大肠杆菌中可复制的DNA区域。 将氨基酮不对称还原酶基因插入穿梭载体中，产生含有载体的转化体，产生氨基酮不对称还原酶和光学活性氨基醇。

公开(公告)号：US20070031948A1

公开(公告)日：2007-02-08

申请号：US10577296

申请日：2004-10-29

申请人： Eitora Yamamura ,  Noboru Fujimoto

发明人： Eitora Yamamura ,  Noboru Fujimoto

IPC分类号： C12P13/00 ,  C07H21/04 ,  C12N9/06 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12P13/008 ,  C12N9/0006 ,  C12N15/70 ,  C12N15/74 ,  C12P13/001

摘要： A shuttle vector is constructed by preparing a DNA region replicable in bacteria belonging to the genus Rhodococcus from a Rhodococcus-derived plasmid having the nucleotide sequence set forth as SEQ ID NO: 73 and a plasmid or its DNA fragment having the nucleotide sequence set forth as SEQ ID NO: 74, and a DNA region replicable in E. coli from an E. coli-derived plasmid or its DNA fragment. An aminoketone asymmetric reductase gene is inserted into the shuttle vector, transformants containing the vector are created, and the aminoketone asymmetric reductase and optically active aminoalcohols are produced.

摘要翻译： 构建穿梭载体，其通过制备可从具有SEQ ID NO：73所示核苷酸序列的红球菌衍生的质粒中的属于红球菌属的细菌中可复制的DNA区域，并且将具有核苷酸序列的质粒或其DNA片段列为 SEQ ID NO：74，以及可从大肠杆菌衍生的质粒或其DNA片段在大肠杆菌中可复制的DNA区域。 将氨基酮不对称还原酶基因插入穿梭载体中，产生含有载体的转化体，产生氨基酮不对称还原酶和光学活性氨基醇。