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公开(公告)号：US4231403A
公开(公告)日：1980-11-04
申请号：US973116
申请日：1978-12-26
CPC分类号： C12M33/04 , Y10T74/1424
摘要： A liquid feeder comprises a motor which drives a cam causing a vertical motion of a drive shaft which is also angularly driven by a cam groove, the drive shaft being effective to cause a three dimensional motion of the discharge port of a single liquid supply tube for injection into a pair of vessels.
摘要翻译： 液体供给器包括驱动凸轮的马达，所述凸轮引起驱动轴的垂直运动，所述驱动轴也由凸轮槽角度地驱动，所述驱动轴有效地引起单个液体供应管的排出口的三维运动 注入一对血管。
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公开(公告)号：US4234023A
公开(公告)日：1980-11-18
申请号：US973117
申请日：1978-12-26
CPC分类号： C12M33/04 , Y10T74/1424
摘要： A liquid feeder comprises a liquid suction and delivery pump which withdraws a liquid such as a culture solution, buffer solution, enzyme solution or the like which is retained in a liquid storage container as refrigerated or at a reduced temperature and delivers it through a flexible tube for injection into a culture vessel located within an incubator. The tube is inserted into an inner pipe and is sealingly secured thereto. The inner pipe is detachably and hermetically mounted in an outer pipe which extends through and is secured to a wall forming an environment isolating layer of the incubator. An eccentric crankpin which is driven by a motor is connected through a link with another crankpin having a greater eccentricity, the latter crankpin being driven for rotation to cause a reciprocatory rotating motion of a driven shaft about its own axis through an angular extent of less than 180.degree.. The motion of the driven shaft is effective to selectively switch the discharge port of a pair of liquid supply tubes to a position over a single vessel. The motor also drives a cam which causes a vertical motion of a drive shaft which is also angularly driven by a cam groove, the drive shaft being effective to cause a three dimensional motion of the discharge port of a single liquid supply tube for injection into a pair of vessels.
摘要翻译： 液体供给器包括一个液体抽吸和输送泵，其将诸如培养液，缓冲溶液，酶溶液等液体冷却或降低的温度保存在液体储存容器中并将其输送通过柔性管 用于注射到位于培养箱内的培养容器中。 将管插入内管并密封地固定在其上。 内管可拆卸地且气密地安装在外管中，该外管延伸穿过并固定到形成培养箱的环境隔离层的壁上。 由马达驱动的偏心曲柄销通过与具有较大偏心率的另一个曲柄销的连杆连接，后者的曲柄销被驱动以旋转，从而使从动轴围绕其自身轴线往复运动，角度范围小于 180度。 从动轴的运动有效地将一对液体供给管的排出口选择性地切换到单个容器上的位置。 电动机还驱动一个凸轮，该凸轮引起驱动轴的垂直运动，驱动轴也由凸轮槽角度地驱动，驱动轴有效地使单个供液管的排出口的三维运动注入到 一对船只。
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公开(公告)号：US4198483A
公开(公告)日：1980-04-15
申请号：US886658
申请日：1978-03-15
申请人： Shinroku Sogi , Makoto Yoshinaga , Toshio Shinohara , Ikuo Tawara
发明人： Shinroku Sogi , Makoto Yoshinaga , Toshio Shinohara , Ikuo Tawara
CPC分类号： B01L3/0275
摘要： A pipette exchange apparatus for use in a system for automatically culturing a biotissue or a cell includes a pipette mounting device for mounting a dividing and injecting means, a pipette supply device and a pipette feeding device.
摘要翻译： 用于自动培养生物组织或细胞的系统中的移液器更换装置包括用于安装分离和注射装置的移液管安装装置，移液管供应装置和移液管供给装置。
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公开(公告)号：US4179339A
公开(公告)日：1979-12-18
申请号：US876894
申请日：1978-02-13
CPC分类号： C12M33/04 , Y10T74/1424
摘要： A liquid feeder comprises a liquid suction and delivery pump which withdraws a liquid such as a culture solution, buffer solution, enzyme solution or the like which is retained in a liquid storage container as refrigerated or at a reduced temperature and delivers it through a flexible tube for injection into a culture vessel located within an incubator. The tube is inserted into an inner pipe and is sealingly secured thereto. The inner pipe is detachably and hermetically mounted in an outer pipe which extends through and is secured to a wall forming an environment isolating layer of the incubator. An eccentric crankpin which is driven by a motor is connected through a link with another crankpin having a greater eccentricity, the latter crankpin being driven for rotation to cause a reciprocatory rotating motion of a driven shaft about its own axis through an angular extent of less than 180.degree.. The motion of the driven shaft is effective to selectively switch the discharge port of a pair of liquid supply tubes to a position over a single vessel. The motor also drives a cam which causes a vertical motion of a drive shaft which is also angularly driven by a cam groove, the drive shaft being effective to cause a three dimensional motion of the discharge port of a single liquid supply tube for injection into a pair of vessels.
摘要翻译： 液体供给器包括一个液体抽吸和输送泵，其将诸如培养液，缓冲溶液，酶溶液等液体冷却或降低的温度保存在液体储存容器中并将其输送通过柔性管 用于注射到位于培养箱内的培养容器中。 将管插入内管并密封地固定在其上。 内管可拆卸地且气密地安装在外管中，该外管延伸穿过并固定到形成培养箱的环境隔离层的壁上。 由马达驱动的偏心曲柄销通过与具有较大偏心率的另一个曲柄销的连杆连接，后者的曲柄销被驱动以旋转，从而使从动轴围绕其自身轴线往复运动，角度范围小于 180度。 从动轴的运动有效地将一对液体供给管的排出口选择性地切换到单个容器上的位置。 电动机还驱动一个凸轮，该凸轮引起驱动轴的垂直运动，驱动轴也由凸轮槽角度地驱动，驱动轴有效地使单个供液管的排出口的三维运动注入到 一对船只。
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5.发明授权
公开(公告)号：US4210724A
公开(公告)日：1980-07-01
申请号：US888036
申请日：1978-03-20
摘要： An apparatus for liquid disposal and distribution in automatic culture system comprises a first turntable carrying a plurality of culture vessels disposed in an array on a common circle thereon, and a second turntable carrying a plurality of centrifuge tubes disposed in an array on a common circle thereof. The apparatus comprises a pipette holding arm which is disposed so that its free end is angularly movable between one of the culture vessels and one of the centrifuge tubes. The free end of the arm has attached thereto a tapered cylinder which detachably receives a pipette. A fixed displacement pump is connected with the cylinder, which is movable along the path of rotation, along which are disposed a pipette holder, a pipette remover and a liquid disposal pot. The apparatus also comprises a rotary and elevating mechanism associated with the arm. In this manner, the apparatus is capable of transferring a specimen being cultured from a culture vessel to a centrifuge tube, distributing grown cells from the tube into culture vessels and disposing unnecessary liquid. In addition, the apparatus is capable of agitating a culturing solution within the culture vessel to render the cells into suspension.
摘要翻译： 一种用于自动培养系统中的液体处理和分配的装置包括：第一转盘，其承载以阵列布置在其上的公共圆上的多个培养容器;以及第二转台，其携带多个在其公共圆上阵列设置的离心管 。 该装置包括移液管保持臂，其设置成使得其自由端在一个培养容器和一个离心管之间成角度地移动。 臂的自由端附接有可拆卸地接收移液管的锥形圆筒。 固定容积泵与气缸连接，该气缸可沿旋转路径移动，沿着该移动路径设置移液管支架，吸移管移除器和液体处理罐。 该装置还包括与臂相关联的旋转和升降机构。 以这种方式，该装置能够将从培养容器中培养的样品转移到离心管中，将生长的细胞从管分配到培养容器中并处理不需要的液体。 此外，该装置能够搅拌培养容器内的培养液以使细胞悬浮。
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公开(公告)号：US4208484A
公开(公告)日：1980-06-17
申请号：US885599
申请日：1978-03-13
CPC分类号： C12M33/10 , B04B5/0421 , B04B2011/046
摘要： An apparatus for handling a centrifuge tube in an automatic culture system includes a centrifuge, above which is disposed a centrifuge tube carrying turntable which is intermittently driven through a given angular increment. Disposed at an equal spacing on and around the periphery of the turntable are centrifuge tube holder mechanisms each carrying a centrifuge tube holder. Also disposed in surrounding relationship with the turntable and at given positions where the latter comes to a stop are a plurality of processing stations including a centrifuge delivery unit which automatically delivers a centrifuge tube into the holder, a liquid disposal unit for disposing unnecessary culture solution from the centrifuge tube which has been subjected to the action of the centrifuge, a discharge unit for discharging a used centrifuge tube subsequent to the completion of the culturing operation, a transfer unit for transferring a centrifuge tube between the centrifuge holder mechanism and a holder receiver located within the centrifuge. As the turntable is incremently driven, the centrifuge tube is successively conveyed through a culture treatment and a centrifuge tube processing position.
摘要翻译： 用于在自动培养系统中处理离心管的装置包括离心机，其上设置有离心管，承载转盘，其间歇地通过给定的角度增量驱动。 在离合器托架机构中，转盘周边等间隔设置有离心管支架机构，每个离心管支架机构都带有离心管支架。 还设置在与转台的周围关系中以及在后者停止的给定位置处的多个处理站包括离心机输送单元，其将离心管自动输送到保持器中，液体处理单元，用于将不必要的培养液从 已经经受离心机作用的离心管，用于在完成培养操作之后排出使用的离心管的排出单元，用于将离心机管转移到离心机保持器机构和位于 在离心机内。 随着转台逐渐驱动，离心管依次通过培养处理和离心管处理位置传送。
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公开(公告)号：US4160699A
公开(公告)日：1979-07-10
申请号：US847553
申请日：1977-11-01
CPC分类号： G01N35/04 , G01N2035/00356 , G01N2035/042 , G01N35/025
摘要： An automatic culture apparatus includes an airtight casing, a culture vessel platform, a culture observation platform and a separation/distribution work platform disposed in layered configuration with suitable vertical spacings between them. A drive system for the apparatus comprises an opening formed centrally in each of the platforms; a culture vessel carrier for conveying a culture vessel to each of the platforms through the openings; a transfer arm for loading and unloading a culture vessel on or from the carrier and any one of the platforms; a first drive mechanism for operating the carrier and the transfer arm; and a second drive mechanism for driving any one of the platforms. The second drive mechanism is mounted hermetically in the casing. By operating suitable drive mechanisms to actuate the various parts, a culture vessel may be automatically transferred and distributed on each of the platforms without causing contamination in a safe and efficient manner, for automatic growth of the medium being cultured under an optimum environment.
摘要翻译： 一种自动培养装置，包括密封壳体，培养皿平台，培养观察平台和分层/分配工作平台，其分层结构具有合适的垂直间距。 该装置的驱动系统包括：在每个平台中央形成的开口; 用于通过开口将培养容器输送到每个平台的培养容器载体; 用于在载体和任何一个平台上装载和卸载培养容器的转移臂; 用于操作所述载体和所述传送臂的第一驱动机构; 以及用于驱动任何一个平台的第二驱动机构。 第二驱动机构气密地安装在壳体中。 通过操作合适的驱动机构来致动各种部件，培养容器可以自动转移和分布在每个平台上，而不会以安全和有效的方式引起污染，从而在最佳环境下培养的培养基的自动生长。
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公开(公告)号：US4090921A
公开(公告)日：1978-05-23
申请号：US707068
申请日：1976-07-20
申请人： Ichiro Sawamura , Shinroku Sogi , Shin-ichi Kamachi , Makoto Yoshinaga , Atsuo Goto , Masao Izawa , Yoshio Nakajima , Nagahiro Gocho , Toshio Shinohara , Shin-ichiro Hattori
发明人： Ichiro Sawamura , Shinroku Sogi , Shin-ichi Kamachi , Makoto Yoshinaga , Atsuo Goto , Masao Izawa , Yoshio Nakajima , Nagahiro Gocho , Toshio Shinohara , Shin-ichiro Hattori
CPC分类号： C12M41/34 , C12M41/36 , C12M41/48 , Y10S435/80 , Y10S435/807 , Y10S435/808 , Y10S435/809
摘要： An apparatus for automatically cultivating tissues or cells of a living body comprises a main body the inside of which is maintained in a predetermined atmosphere by an atmosphere control means. A container containing the tissues or cells and a nutrient solution is disposed in the main body and cultivated or multiplied tissues or cells in the container divided and fed for subculture into a plurality of empty cultivating containers disposed in the main body by an operating means which performs necessary operations for subculture. The atmosphere control means and the operating means are totally controlled by an apparatus control means.
摘要翻译： 用于自动培养生物体的组织或细胞的装置包括通过气氛控制装置将其内部保持在预定的气氛中的主体。 在主体中设置容纳组织或细胞的容器以及营养液，并通过操作装置进行分割并供给传代培养的容器中的组织或细胞进行培养或繁殖成为设置在主体中的多个空栽培容器 亚文化的必要操作。 气氛控制装置和操作装置由装置控制装置完全控制。
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9.发明授权Method for automatically and successively cultivating tissues or cells of a body 失效自动连续培养身体组织或细胞的方法
公开(公告)号：US4154652A
公开(公告)日：1979-05-15
申请号：US694643
申请日：1976-06-10
申请人： Ichiro Sawamura , Shinroku Sogi , Shin-ichi Kamachi , Makoto Yoshinaga , Atsuo Goto , Masao Izawa , Yoshio Nakajima , Nagahiro Gocho , Toshio Shinohara , Shinichiro Hattori
发明人： Ichiro Sawamura , Shinroku Sogi , Shin-ichi Kamachi , Makoto Yoshinaga , Atsuo Goto , Masao Izawa , Yoshio Nakajima , Nagahiro Gocho , Toshio Shinohara , Shinichiro Hattori
摘要： Tissues or cells of a living body, especially a human body, are automatically and successively cultivated in a nutrient solution filled culture container disposed in a gas-sealed culture box by detecting, within the culture box, a multiplication condition of the tissues or cells and culture conditions which are represented by temperature, humidity and gaseous atmosphere in the culture box and pH value of the nutrient solution, controlling the culture conditions and apparatus for subculture of the tissues or cells within the culture box in accordance with the information detected.
摘要翻译： 通过在培养箱内检测组织或细胞的倍增条件，将生物体特别是人体的组织或细胞自动依次培养在设置在气体密封培养箱中的营养液填充培养容器中，以及 培养条件由培养箱中的温度，湿度和气体气氛以及营养液的pH值表示，根据检测到的信息控制培养条件和培养箱内组织或细胞传代培养的装置。
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公开(公告)号：US06686312B1
公开(公告)日：2004-02-03
申请号：US09522690
申请日：2000-03-10
申请人： Mikio Aramata , Masaaki Furuya , Yoshihiro Shirota , Akio Muraida , Susumu Ueno , Toshio Shinohara
发明人： Mikio Aramata , Masaaki Furuya , Yoshihiro Shirota , Akio Muraida , Susumu Ueno , Toshio Shinohara
IPC分类号： B01J2370
摘要： In the synthesis of organohalosilanes by the direct process of reacting an organic halogen with metallic silicon powder, a metallic copper catalyst in the form of a thermally active metallic copper powder having large strain energy is used.
摘要翻译： 在通过使有机卤素与金属硅粉反应的直接方法合成有机卤代硅烷中，使用具有大应变能的热活性金属铜粉形式的金属铜催化剂。
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