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    CELL MASS CAPABLE OF SERVING AS A PRIMITIVE ORGAN-LIKE STRUCTURE COMPRISED OF A PLURALITY OF CELL TYPES OF SOMATIC ORIGIN
    2.
    发明申请
    CELL MASS CAPABLE OF SERVING AS A PRIMITIVE ORGAN-LIKE STRUCTURE COMPRISED OF A PLURALITY OF CELL TYPES OF SOMATIC ORIGIN 审中-公开
    作为主要的类似结构的细胞质量可以作为包含多种细胞类型的原始原始结构

    公开(公告)号：US20140106432A1

    公开(公告)日：2014-04-17

    申请号：US14030449

    申请日：2013-09-18

    申请人： Shiseido Company, Ltd.

    发明人： Shigeyoshi Fujiwara ,  Jiro Kishimoto ,  Tsutomu Soma

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0697 ,  C12N5/0625 ,  C12N5/0627 ,  C12N5/0629 ,  C12N5/0666 ,  C12N2501/40 ,  C12N2501/415

    摘要： The present invention provides a method of producing a cell mass capable of serving as a primitive organ-like structure comprised of a plurality of somatic cell types of somatic origin, comprising: preparing cultures containing the plurality of types of somatic cells; mixing the plurality of types of somatic cell cultures followed by adding a Wnt signal activator to the mixed cell culture; subjecting the culture containing the Wnt signal activator to non-plate contact culturing over a predetermined time period; and replacing the medium of the culture cultured by the non-plate contact culturing with medium not containing Wnt signal activator and further culturing for a predetermined time period; wherein, at least one type of the plurality of somatic cells is maintained in an undifferentiated state.

    摘要翻译： 本发明提供一种制备能够作为由体细胞起源的多种体细胞类型构成的原始器官状结构的细胞块的方法，其包括：制备含有多种体细胞的培养物; 混合多种类型的体细胞培养物，然后向混合细胞培养物中加入Wnt信号激活剂; 使含有Wnt信号激活剂的培养物在预定时间段内进行非平板接触培养; 并用不含Wnt信号激活剂的培养基替换由非平板接触培养培养的培养基，并进一步培养预定时间; 其中，至少一种类型的所述多个体细胞维持在未分化状态。

    Cell mass capable of serving as a primitive organ-like structure comprised of a plurality of cell types of somatic origin
    4.
    发明授权
    Cell mass capable of serving as a primitive organ-like structure comprised of a plurality of cell types of somatic origin 有权

    公开(公告)号：US10344266B2

    公开(公告)日：2019-07-09

    申请号：US14788925

    申请日：2015-07-01

    申请人： Shiseido Company, Ltd.

    发明人： Shigeyoshi Fujiwara ,  Jiro Kishimoto ,  Tsutomu Soma

    IPC分类号： C12N5/00 ,  C12N5/071 ,  C12N5/0775

    摘要： The present invention provides a method of producing a cell mass capable of serving as a primitive organ-like structure comprised of a plurality of somatic cell types of somatic origin, comprising: preparing cultures containing the plurality of types of somatic cells; mixing the plurality of types of somatic cell cultures followed by adding a Wnt signal activator to the mixed cell culture; subjecting the culture containing the Wnt signal activator to non-plate contact culturing over a predetermined time period; and replacing the medium of the culture cultured by the non-plate contact culturing with medium not containing Wnt signal activator and further culturing for a predetermined time period; wherein, at least one type of the plurality of somatic cells is maintained in an undifferentiated state.