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公开(公告)号：US5744325A
公开(公告)日：1998-04-28
申请号：US806613
申请日：1997-02-26
CPC分类号： C12N9/80
摘要： A process for producing N-acetylglucosamine-6-phosphate deacetylase comprising incubating a microorganism which belongs to the marine psychrotrophic bacterium Vibrio sp. and recovering the N-acetylglucosamine-6-phosphate deacetylase from the culture thus obtained; N-acetylglucosamine-6-phosphate deacetylase; and a bacterium belonging to the marine psychrotrophic bacterium Vibrio sp.
摘要翻译： 一种生产N-乙酰葡糖胺-6-磷酸脱乙酰酶的方法，包括将属于海洋营养型细菌弧菌属的微生物孵育。 从得到的培养物中回收N-乙酰氨基葡萄糖-6-磷酸脱乙酰酶; N-乙酰葡糖胺-6-磷酸脱乙酰酶; 和属于海洋营养型细菌弧菌属的细菌。
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公开(公告)号：US06352850B1
公开(公告)日：2002-03-05
申请号：US09661808
申请日：2000-09-14
IPC分类号： C12N924
CPC分类号： C12Y302/01014 , C12N9/2442
摘要： Chitinase with the following physico-chemical properties 1 to 6: 1. action: random cutting of the &bgr;-1,4 bond of chitin to generate the tetramer and dimer of Nacetylglucosamine; 2. optimum pH: 6.5 to 10.4; 3. stable pH: 7.0 to 9.0; 4. optimum temperature: 37° C.; 5. active temperature range: 4 to 60° C.; and 6. thermal stability: 60% or more of the initial activity as retained even after heating at 40° C. and pH 8.0 for 30 minutes; and a method for producing chitinase, comprising culturing a chitinase-generating bacterium reacting with chitin to generate the N-acetylglucosamine oligomer and collecting chitinase from the culture.
摘要翻译： 具有以下物理化学性质的几丁质酶1至6：1。 作用：随机切割几丁质的β-1,4键以产生N-乙酰基葡萄糖胺的四聚体和二聚体; 2。 最适pH：6.5〜10.4; 3。 稳定pH：7.0〜9.0; 4。 最佳温度：37℃; 5。 有效温度范围：4〜60℃。 和6。 热稳定性：即使在40℃，pH8.0加热30分钟后，初始活性也保持60％以上。 以及生产几丁质酶的方法，包括培养与几丁质反应的几丁质酶生成细菌以产生N-乙酰葡糖胺低聚物并从培养物中收集几丁质酶。
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公开(公告)号：US5731184A
公开(公告)日：1998-03-24
申请号：US609107
申请日：1996-02-29
CPC分类号： C12N9/80
摘要： A process for producing N-acetyl-D-glucosamine deacetylase which comprises incubating a microorganism belonging to the genus Alteromonas and recovering N-acetyl-D-glucosamine deacetylase from the culture thus obtained.
摘要翻译： 一种N-乙酰基-D-葡糖胺脱乙酰酶的制造方法，其特征在于，将从所得培养物中回收N-乙酰基-D-葡糖胺脱乙酰酶的方法，培养属于Alteromonas属的微生物。
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公开(公告)号：US5702939A
公开(公告)日：1997-12-30
申请号：US612491
申请日：1996-03-07
申请人： Shizu Fujishima , Naoko Yamano
发明人： Shizu Fujishima , Naoko Yamano
CPC分类号： C12N9/90 , Y10S435/909
摘要： A glucosamine 6-phosphate deaminase having specific physicochemical properties. A process for producing the glucosamine 6-phosphate deaminase which comprises incubating a microorganism belonging to the genus Vibrio and harvesting the glucosamine 6-phosphate deaminase from the culture thus obtained.
摘要翻译： 具有特定物理化学性质的葡糖胺6-磷酸脱氨酶。 一种生产葡糖胺-6-磷酸脱氨酶的方法，其包括温育属于弧菌属的微生物并从所得培养物中收获葡糖胺-6-磷酸脱氨酶。
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5.依法登记的发明
公开(公告)号：USH1453H
公开(公告)日：1995-06-06
申请号：US154350
申请日：1993-11-18
申请人： Naoko Yamano , Shizu Fujishima , Ryutarou Tanaka
发明人： Naoko Yamano , Shizu Fujishima , Ryutarou Tanaka
摘要： There are provided N-acetyl-D-glucosamine deacetylase capable of hydrolyzing acetamide group of N-acetyl-D-glucosamine to produce D-glucosamine and acetic acid, which has the following physicochemical properties:(1) Substrate specificity: N-acetyl-D-glucosamine monomer, not the oligomer or polymer thereof;(2) Optimal pH: 7.8-8.2 at 37.degree. C.;(3) Stable pH: 6.0-9.0 at 45.degree. C.;(4) Optimal temperature: 28.degree.-39.degree. C.;(5) Molecular weight: 70,000-200,000 as determined using TSK-gel G-3000 SW column,and a process for preparing the same using a microorganism belonging to Vibrio sp.
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公开(公告)号：US5252468A
公开(公告)日：1993-10-12
申请号：US842304
申请日：1992-02-26
申请人： Shizu Fujishima , Fumiko Yaku , Ryutarou Tanaka , Einosuke Muraki , Naoko Yamano
发明人： Shizu Fujishima , Fumiko Yaku , Ryutarou Tanaka , Einosuke Muraki , Naoko Yamano
CPC分类号： C12N9/80
摘要： A process for producing deacetylase which comprises incubating a deacetylase-producing bacterium belonging to Vibrio cholerae in a IFO 15429 culture medium containing a carbon source, a nitrogen source, an inorganic salt and an inducer for producing the deacetylase and isolating the deacetylase from cells separated from said culture medium.
摘要翻译： 一种生产脱乙酰酶的方法，其包括在含有碳源，氮源，无机盐和用于制备脱乙酰酶的诱导剂的IFO 15429培养基中温育属于霍乱弧菌的脱乙酰酶产生细菌，并从脱离 说培养基。
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公开(公告)号：US4713334A
公开(公告)日：1987-12-15
申请号：US843809
申请日：1986-03-27
申请人： Shizu Fujishima , Fumiko Yaku , Tetsuo Koshijima
发明人： Shizu Fujishima , Fumiko Yaku , Tetsuo Koshijima
CPC分类号： C12P19/14 , Y10S435/813
摘要： A process for the saccharification of celluloses is provided. More particularly, a solution obtained after the degradative saccharification of cellulosic materials with cellulases is separated into a liquid portion containing saccharides and a solid matter. Cellulases are recovered from said liquid portion containing saccharides, while said solid matter is treated with an aqueous pH-buffered solution, or an aqueous solution, alcoholic aqueous solution or aqueous pH-buffered solution of polysaccharides or oligosaccharides, or aqueous or aqueous pH-buffered solution of alcohols to recover the cellulases in the solid matter. With the use of this solid matter, newly added cellulosic materials are degradatively saccharified in an aqueous solution or the above-mentioned solutions.
摘要翻译： 提供了一种纤维素糖化方法。 更具体地说，在用纤维素酶对纤维素材料进行降解糖化后获得的溶液被分离成含有糖和固体物质的液体部分。 从含有糖类的所述液体部分回收纤维素酶，而所述固体物质用水性pH缓冲溶液或水溶液，醇水溶液或多糖或低聚糖的pH缓冲溶液水溶液或pH缓冲液 醇的溶液以回收固体物质中的纤维素酶。 通过使用该固体物质，新添加的纤维素材料在水溶液或上述溶液中降解糖化。
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公开(公告)号：US4970150A
公开(公告)日：1990-11-13
申请号：US363802
申请日：1989-06-09
申请人： Fumiko Yaku , Ryutarou Tanaka , Einosuke Muraki , Shizu Fujishima , Masaru Miya
发明人： Fumiko Yaku , Ryutarou Tanaka , Einosuke Muraki , Shizu Fujishima , Masaru Miya
CPC分类号： C12P19/04 , Y10S435/917 , Y10S435/945
摘要： A process for preparing a low-molecular weight chitosan which comprises adding chitosan to water containing a monobasic acid to prepare an intimate chitosan-water mixture and treating said mixture with cellulase.
摘要翻译： 一种制备低分子量壳聚糖的方法，其包括向含有一元酸的水中加入壳聚糖以制备紧密的壳聚糖 - 水混合物并用纤维素酶处理所述混合物。
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公开(公告)号：US4746611A
公开(公告)日：1988-05-24
申请号：US814478
申请日：1985-12-30
申请人： Shizu Fujishima , Fumiko Yaku , Einosuke Muraki
发明人： Shizu Fujishima , Fumiko Yaku , Einosuke Muraki
CPC分类号： C12N9/2437 , C12P19/14 , C12Y302/01004 , Y10S435/813 , Y10S435/913
摘要： A cellulosic material is saccharified by cellulases and the resulting saccharified solution is acidified. Subsequently chitosan and/or partially deacetylated chitin are dissolved therein and the obtained solution is alkalified. Thus the cellulases are absorbed by the chitosan and/or partially deacetylated chitin. Further a cellulosic material is saccharified by at least one cellulase originating from a fungus belonging to the genus Aspergillus and at least either chitosan and/or partially deacetylated chitin are added to the resulting saccharified solution to thereby adsorb the cellulase by the chitosan and/or partially deacetylated chitin.
摘要翻译： 纤维素材料被纤维素酶糖化，所得糖化溶液酸化。 随后将壳聚糖和/或部分脱乙酰化几丁质溶解在其中，将所得溶液碱化。 因此，纤维素酶被壳聚糖和/或部分脱乙酰化几丁质所吸收。 此外，纤维素材料被来自属于曲霉属的真菌的至少一种纤维素酶糖化，并且至少将壳聚糖和/或部分脱乙酰化几丁质加入到所得糖化溶液中，从而通过壳聚糖和/或部分地吸附纤维素酶 脱乙酰壳多糖
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