公开(公告)号：US20240280589A1

公开(公告)日：2024-08-22

申请号：US18391104

申请日：2023-12-20

申请人： Sinai Health System ,  The Hospital for Sick Children

发明人： Isabella Caniggia ,  Martin Post ,  Sruthi Alahari

IPC分类号： G01N33/68

CPC分类号： G01N33/689 ,  G01N2333/78 ,  G01N2333/90245 ,  G01N2800/368

摘要： A method and kit for predicting preeclampsia is provided. Levels of Jumonji C domain containing protein 6 (JMJD6) and fibronectin are measured in placenta-derived small extracellular vesicles isolated from a subject. The levels of JMJD6 and fibronectin are compared to a non-preeclamptic control. If levels of JMJD6 are depressed and levels of fibronectin are elevated, the subject may be monitored or treated for preeclampsia.

公开(公告)号：US20200171085A1

公开(公告)日：2020-06-04

申请号：US16614994

申请日：2018-05-18

申请人： The Hospital for Sick Children

发明人： Theo Moraes ,  Martin Post ,  Yuchen Cen ,  Michael Litvack

IPC分类号： A61K35/15 ,  C12N5/0786 ,  A61K9/00 ,  A61P31/14

摘要： A method of treating a respiratory viral infection in a mammal is provided. The method comprises administering to the mammal a therapeutically effective amount of alveolar-like macrophages, or an anti-viral factor produced by alveolar-like macrophages.

公开(公告)号：US20220162556A1

公开(公告)日：2022-05-26

申请号：US17598593

申请日：2020-03-27

申请人： The Hospital for Sick Children

发明人： Sheena Bouch ,  Michael Litvack ,  Martin Post

IPC分类号： C12N5/0789 ,  C12N5/0786 ,  C12N5/071

摘要： A method of differentiating pluripotent stem cells into hemangioblasts comprising incubating the pluripotent stem cells in a first serum-free differentiation medium comprising bone morphogenetic protein 4 (BMP4), vascular endothelial growth factor (VEGF) and stem cell factor (SCF) to induce differentiation of the pluripotent stem cells into hemangioblasts or hemangioblast-containing embryoid bodies is provided. The hemangioblasts or embryoid bodies may be cultured in a second differentiation medium comprising at el least granulocyte-macrophage colony stimulating factor (GM-CSF), macrophage colony stimulating factor (M-CSF) and interleukin-3 (IL-3) for a period of time sufficient to generate alveolar-like macrophages.

公开(公告)号：US20210301256A1

公开(公告)日：2021-09-30

申请号：US17316060

申请日：2021-05-10

申请人： The Hospital for Sick Children

发明人： Martin Post ,  Michael Litvack

IPC分类号： C12N5/0786 ,  C07K14/81 ,  C07K14/54 ,  A61K35/15

摘要： Alveolar-like macrophages and a method for generating alveolar-like macrophages from hemangioblasts is provided. The method comprises the steps of: i) culturing the hemangioblasts in a hematopoietic-inducing medium comprising vascular endothelial growth factor (VEGF), stem cell factor (SCF) and interleukin-3 (IL-3) for a sufficient period of time to generate macrophages, and ii) culturing the macrophages in an alveolar macrophage-inducing medium comprising granulocyte macrophage colony stimulating factor (GM-CSF), and optionally macrophage colony stimulating factor (M-CSF), under suitable conditions and for a sufficient period of time to yield alveolar-like macrophages.

公开(公告)号：US11001806B2

公开(公告)日：2021-05-11

申请号：US15672432

申请日：2017-08-09

申请人： The Hospital for Sick Children

发明人： Martin Post ,  Michael Litvack

IPC分类号： C12N5/00 ,  C12N5/0786 ,  A61K35/15 ,  C12N5/0735

摘要： Alveolar-like macrophages and a method for generating alveolar-like macrophages from hemangioblasts is provided. The method comprises the steps of: i) culturing the hemangioblasts in a hematopoietic-inducing medium comprising vascular endothelial growth factor (VEGF), stem cell factor (SCF) and interleukin-3 (IL-3) for a sufficient period of time to generate macrophages, and ii) culturing the macrophages in an alveolar macrophage-inducing medium comprising granulocyte macrophage colony stimulating factor (GM-CSF), and optionally macrophage colony stimulating factor (M-CSF), under suitable conditions and for a sufficient period of time to yield alveolar-like macrophages.

公开(公告)号：US20170335282A1

公开(公告)日：2017-11-23

申请号：US15672432

申请日：2017-08-09

申请人： The Hospital for Sick Children

发明人： Martin Post ,  Michael Litvack

IPC分类号： C12N5/0786 ,  A61K35/15 ,  C12N5/0735

CPC分类号： C12N5/0645 ,  A61K35/15 ,  C12N5/0606 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2501/33 ,  C12N2506/02 ,  C12N2506/11 ,  C12N2506/45

摘要： Alveolar-like macrophages and a method for generating alveolar-like macrophages from hemangioblasts is provided. The method comprises the steps of: i) culturing the hemangioblasts in a hematopoietic-inducing medium comprising vascular endothelial growth factor (VEGF), stem cell factor (SCF) and interleukin-3 (IL-3) for a sufficient period of time to generate macrophages, and ii) culturing the macrophages in an alveolar macrophage-inducing medium comprising granulocyte macrophage colony stimulating factor (GM-CSF), and optionally macrophage colony stimulating factor (M-CSF), under suitable conditions and for a sufficient period of time to yield alveolar-like macrophages.