公开(公告)号：US10247655B2

公开(公告)日：2019-04-02

申请号：US14907867

申请日：2014-07-30

Applicant: Sphere Fluidics Limited

Inventor： Clive A. Smith ,  Xin Li ,  Karine Enesa ,  Xin Liu ,  Frank F. Craig ,  Graeme Whyte

IPC: G01N15/14 ,  B01L3/00 ,  C12Q1/04 ,  C12Q1/06 ,  G01N21/49 ,  G01N15/00

Abstract: A method of label-free cell or particle sorting in a microfluidic device includes providing a stream of aqueous droplets in oil in a channel of the microfluidic device, wherein at least some of the droplets include cells or particles and illuminating the stream from a first direction. Then, one detects scattered light from cells or particles within said aqueous droplets in a second direction. Next, one determines a number of the cells or particles in each droplet from the scattered light and sorts the aqueous droplets into differentiated streams. Notably, the refractive index of said oil is modified to closely match a refractive index of the aqueous droplets to reduce light scattered from boundaries of the droplets. A volume of the droplets can also controlled.

公开(公告)号：US09513207B2

公开(公告)日：2016-12-06

申请号：US14907936

申请日：2014-07-30

Applicant: Sphere Fluidics Limited

Inventor： Clive A. Smith ,  Xin Li ,  Karine Enesa ,  Xin Liu ,  Frank F. Craig ,  Graeme Whyte

IPC: A61K9/00 ,  G01N15/14 ,  B01L3/00

CPC classification number: G01N15/1484 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  G01N2015/1481 ,  G01N2015/149

Abstract: The present invention provides an emulsion for sorting droplets in a microfluidic device, the emulsion comprising: a discontinuous aqueous phase; and a continuous oil phase; wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.