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公开(公告)号：US10247655B2
公开(公告)日：2019-04-02
申请号：US14907867
申请日：2014-07-30
Applicant: Sphere Fluidics Limited
Inventor： Clive A. Smith , Xin Li , Karine Enesa , Xin Liu , Frank F. Craig , Graeme Whyte
Abstract: A method of label-free cell or particle sorting in a microfluidic device includes providing a stream of aqueous droplets in oil in a channel of the microfluidic device, wherein at least some of the droplets include cells or particles and illuminating the stream from a first direction. Then, one detects scattered light from cells or particles within said aqueous droplets in a second direction. Next, one determines a number of the cells or particles in each droplet from the scattered light and sorts the aqueous droplets into differentiated streams. Notably, the refractive index of said oil is modified to closely match a refractive index of the aqueous droplets to reduce light scattered from boundaries of the droplets. A volume of the droplets can also controlled.
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公开(公告)号：US09513207B2
公开(公告)日：2016-12-06
申请号：US14907936
申请日：2014-07-30
Applicant: Sphere Fluidics Limited
Inventor： Clive A. Smith , Xin Li , Karine Enesa , Xin Liu , Frank F. Craig , Graeme Whyte
CPC classification number: G01N15/1484 , B01L3/502784 , B01L2200/0652 , B01L2200/0673 , G01N2015/1481 , G01N2015/149
Abstract: The present invention provides an emulsion for sorting droplets in a microfluidic device, the emulsion comprising: a discontinuous aqueous phase; and a continuous oil phase; wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.
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公开(公告)号：US20160252446A1
公开(公告)日：2016-09-01
申请号：US14907867
申请日：2014-07-30
Applicant: Sphere Fluidics Limited
Inventor： Clive A. SMITH , Xin LI , Karine Enesa , Xin LIU , Frank F. CRAIG , Graeme WHYTE
IPC: G01N15/14
CPC classification number: G01N15/1434 , B01L3/502784 , B01L2200/0652 , B01L2200/0673 , C12Q1/04 , C12Q1/06 , G01N15/1425 , G01N21/49 , G01N2015/0065 , G01N2015/149 , Y10T436/2575
Abstract: We describe a method of label-free cell and/or particle sorting in a microfluidic device, the method comprising; providing a stream of aqueous droplets in oil in a channel of said microfluidic device, wherein at least some of said droplets include cells and/or particles illuminating said stream from a first direction; detecting, for example, scattered light from cells and/or particles within said aqueous droplets in a second direction; determining, for example, a number of said cells and/or particles in each droplet from the scattered light; and sorting said aqueous droplets into one of a plurality of differentiated streams responsive to said determined number. The method further comprises modifying a refractive index of said oil to more closely match a refractive index of said aqueous droplets to reduce light scattered from boundaries of said droplets. Optionally a volume of the droplets is also controlled. In embodiments the oil is fluorous oil. We describe examples of preferred refractive index modifying compounds.
Abstract translation: 我们描述了在微流体装置中无标记细胞和/或颗粒分选的方法，该方法包括： 在所述微流体装置的通道中提供油中的水滴液滴，其中至少一些所述液滴包括从第一方向照射所述流的细胞和/或颗粒; 从第二方向检测来自所述水滴中的细胞和/或颗粒的散射光; 确定例如来自散射光的每个液滴中的所述细胞和/或颗粒的数量; 以及响应于所述确定的数量将所述水滴分选成多个分化流中的一个。 该方法还包括改变所述油的折射率以更紧密地匹配所述水滴的折射率，以减少从所述液滴的边界散射的光。 任选地，还控制一定体积的液滴。 在实施方案中，油是氟油。 我们描述优选折射率修饰化合物的实例。
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