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公开(公告)号:US07122178B1
公开(公告)日:2006-10-17
申请号:US10030411
申请日:2000-07-07
申请人: Paul Simmons , Andrew Zannettino , Stan Gronthos
发明人: Paul Simmons , Andrew Zannettino , Stan Gronthos
CPC分类号: C12N5/0663 , A61K2035/124 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 富含间充质前体的方法包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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公开(公告)号:US20060008452A1
公开(公告)日:2006-01-12
申请号:US11178920
申请日:2005-07-11
申请人: Paul Simmons , Andrew Zannettino , Stan Gronthos
发明人: Paul Simmons , Andrew Zannettino , Stan Gronthos
CPC分类号: C12N5/0663 , A61K2035/124 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
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公开(公告)号:US20050281790A1
公开(公告)日:2005-12-22
申请号:US11169875
申请日:2005-06-29
申请人: Paul Simmons , Andrew Zannettino , Stan Gronthos
发明人: Paul Simmons , Andrew Zannettino , Stan Gronthos
IPC分类号: A61K35/12 , C12N5/0775 , A61K45/00 , C12N5/08
CPC分类号: C12N5/0663 , A61K2035/124 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 富含间充质前体的方法包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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公开(公告)号:US08158118B2
公开(公告)日:2012-04-17
申请号:US11169875
申请日:2005-06-29
申请人: Paul Simmons , Andrew Zannettino , Stan Gronthos
发明人: Paul Simmons , Andrew Zannettino , Stan Gronthos
CPC分类号: C12N5/0663 , A61K2035/124 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 富含间充质前体的方法包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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公开(公告)号:US08546138B2
公开(公告)日:2013-10-01
申请号:US13270863
申请日:2011-10-11
申请人: Stan Gronthos , Andrew Zannettino , Paul Simmons
发明人: Stan Gronthos , Andrew Zannettino , Paul Simmons
CPC分类号: A61K35/28 , A61K47/48861 , A61K47/6923 , A61K48/00 , A61K2035/124 , C12N5/0663 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilising MACS recognising expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilising two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 一种富含间充质前体细胞的方法,包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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公开(公告)号:US20120276553A1
公开(公告)日:2012-11-01
申请号:US13270863
申请日:2011-10-11
申请人: Stan Gronthos , Andrew Zannettino , Paul Simmons
发明人: Stan Gronthos , Andrew Zannettino , Paul Simmons
IPC分类号: C12N5/0775 , G01N33/577 , C12N15/85 , G01N33/566
CPC分类号: A61K35/28 , A61K47/48861 , A61K47/6923 , A61K48/00 , A61K2035/124 , C12N5/0663 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilising MACS recognising expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilising two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 一种富含间充质前体细胞的方法,包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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公开(公告)号:US08062675B2
公开(公告)日:2011-11-22
申请号:US12660003
申请日:2010-02-17
申请人: Stan Gronthos , Andrew Zannettino , Paul Simmons
发明人: Stan Gronthos , Andrew Zannettino , Paul Simmons
IPC分类号: A61K35/26
CPC分类号: A61K35/28 , A61K47/48861 , A61K47/6923 , A61K48/00 , A61K2035/124 , C12N5/0663 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilising MACS recognising expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilising two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 一种富含间充质前体细胞的方法,包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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公开(公告)号:US20100330045A1
公开(公告)日:2010-12-30
申请号:US12660003
申请日:2010-02-17
申请人: Stan Gronthos , Andrew Zannettino , Paul Simmons
发明人: Stan Gronthos , Andrew Zannettino , Paul Simmons
CPC分类号: A61K35/28 , A61K47/48861 , A61K47/6923 , A61K48/00 , A61K2035/124 , C12N5/0663 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilising MACS recognising expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilising two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 一种富含间充质前体细胞的方法,包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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公开(公告)号:US07670628B2
公开(公告)日:2010-03-02
申请号:US10813747
申请日:2004-03-29
申请人: Paul Simmons , Andrew Zannettino , Stan Gronthos
发明人: Paul Simmons , Andrew Zannettino , Stan Gronthos
IPC分类号: A61K35/26
CPC分类号: A61K35/28 , C12N5/0663 , C12N5/0691 , C12N2500/42 , C12N2501/39 , C12N2506/1353
摘要: A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 一种富含间充质前体细胞的方法,包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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公开(公告)号:US07399632B2
公开(公告)日:2008-07-15
申请号:US11178920
申请日:2005-07-11
申请人: Paul Simmons , Andrew Zannettino , Stan Gronthos
发明人: Paul Simmons , Andrew Zannettino , Stan Gronthos
CPC分类号: C12N5/0663 , A61K2035/124 , C12N2500/42 , C12N2501/39
摘要: A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
摘要翻译: 富含间充质前体的方法包括基于至少两种标记物富集细胞的步骤。 标记可能是i)对间质前体细胞特异性标志物的存在,ii)不存在对分化的间充质细胞特异性的标记,或iii)对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤,随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤,以及 VCAM-1的表达。
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