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1.发明授权Stabilized, NAD(P)H-dependent, soluble nitrate reductase, a process for the preparation thereof and a reagent containing it 失效稳定的NAD（P）H依赖性，可溶性硝酸还原酶，其制备方法和含有它的试剂
公开(公告)号：US5169758A
公开(公告)日：1992-12-08
申请号：US561358
申请日：1990-08-01
CPC分类号： C12N9/0036 , C12N9/96 , C12Q1/26 , C12Y107/01002
摘要： The present invention provides a stabilized, NAD(P)H-dependent, soluble nitrate reductase of the assimilatory type, characterized by a molecular weight of about 90,000 D in the case of electrophoresis in the presence of sodium dodecyl sulphate and a residual activity after 3 weeks at 35.degree. C. of more than 60%, obtainable by preparing a suspension of the comminuted starting material in tris/tartaric acid buffer (pH 7-8.5), adding soluble polyethyleneimine thereto in such an amount that 10 to 20% of the nitrate reductase activity passes into the precipitation obtained, separating off the precipitate and working up the supernatant according to conventional biochemical methods of fractionation in the above-mentioned digestion buffer, dialyzing the enzyme solution obtained and lyophilizing in a zwitterionic buffer.
摘要翻译： 本发明提供了一种稳定的NAD（P）H依赖的可溶性硝酸还原酶，其特征在于在十二烷基硫酸钠存在下电泳时的分子量约为90,000D，并且在3 通过制备在三元/酒石酸缓冲液（pH7-8.5）中粉碎的起始材料的悬浮液，可以将其中的可溶性聚乙烯亚胺加入到其中，其量为10至20％ 硝酸还原酶活性进入所得沉淀物，根据常规的上述消化缓冲液中的分级分离方法分离出沉淀物并除去上清液，透析所得酶解液并在两性离子缓冲液中冻干。
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2.发明授权
公开(公告)号：US6068971A
公开(公告)日：2000-05-30
申请号：US907733
申请日：1992-06-22
IPC分类号： H01L21/36 , C12Q1/00 , C12Q1/25 , C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/42 , C12Q1/48 , C12Q1/527 , C12Q1/54 , G01N20060101 , G01N31/22 , G01N33/50 , G01N33/52 , G01N33/64 , G01N33/84
CPC分类号： G01N33/64 , C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/84 , C12Q2334/10 , C12Q2337/12 , G01N2333/9121 , G01N2333/924 , Y10S435/962
摘要： A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.
摘要翻译： 用于测定流体中离子的方法和试剂，其中测量这些离子对酶活性的影响。 离子例如是钠，钾，钙，镁，锰，锂，铅，锌，铜，铁或其它重金属或含有氯化物，碳酸氢盐，质子，铵的非金属离子和产生铵的物质。 所使用的酶可以是转移酶，水解酶，氧化还原酶或裂合酶。 一个基本特征是通过用结合剂掩蔽干扰离子来排除离子的干扰的方法。
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公开(公告)号：US5501958A
公开(公告)日：1996-03-26
申请号：US308823
申请日：1994-09-19
IPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q1/00 , G01N33/20 , G01N33/566
CPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q2334/10 , C12Q2337/12 , G01N2333/9121 , G01N2333/924 , Y10S435/962
摘要： A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.
摘要翻译： 用于测定流体中离子的方法和试剂，其中测量这些离子对酶活性的影响。 离子例如是钠，钾，钙，镁，锰，锂，铅，锌，铜，铁或其它重金属或含有氯化物，碳酸氢盐，质子，铵的非金属离子和产生铵的物质。 所使用的酶可以是转移酶，水解酶，氧化还原酶或裂合酶。 一个基本特征是通过用结合剂掩蔽干扰离子来排除离子的干扰的方法。
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4.发明授权Enzymatic determination of analyte ions in fluids by optimizing measurement levels 失效通过优化测量水平来酶测定流体中的分析物离子
公开(公告)号：US5380649A
公开(公告)日：1995-01-10
申请号：US907731
申请日：1992-06-22
IPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q1/00
CPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q2334/10 , C12Q2337/12 , G01N2333/9121 , G01N2333/924 , Y10S435/962
摘要： A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.
摘要翻译： 用于测定流体中离子的方法和试剂，其中测量这些离子对酶活性的影响。 离子例如是钠，钾，钙，镁，锰，锂，铅，锌，铜，铁或其它重金属或含有氯化物，碳酸氢盐，质子，铵的非金属离子和产生铵的物质。 所使用的酶可以是转移酶，水解酶，氧化还原酶或裂合酶。 一个基本特征是通过用结合剂掩蔽干扰离子来排除离子的干扰的方法。
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公开(公告)号：US5384247A
公开(公告)日：1995-01-24
申请号：US907736
申请日：1992-06-22
IPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q1/68
CPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q2334/10 , C12Q2337/12 , G01N2333/9121 , G01N2333/924 , Y10S435/962
摘要： A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.
摘要翻译： 用于测定流体中离子的方法和试剂，其中测量这些离子对酶活性的影响。 离子例如是钠，钾，钙，镁，锰，锂，铅，锌，铜，铁或其它重金属或含有氯化物，碳酸氢盐，质子，铵的非金属离子和产生铵的物质。 所使用的酶可以是转移酶，水解酶，氧化还原酶或裂合酶。 一个基本特征是通过用结合剂掩蔽干扰离子来排除离子的干扰的方法。
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6.发明授权Determination of ions in fluids by a process involving displacement of indicator ions 失效通过涉及指示离子位移的方法测定流体中的离子
公开(公告)号：US5384246A
公开(公告)日：1995-01-24
申请号：US907732
申请日：1992-06-22
IPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q1/00
CPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q2334/10 , C12Q2337/12 , G01N2333/9121 , G01N2333/924 , Y10S435/962
摘要： A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.
摘要翻译： 用于测定流体中离子的方法和试剂，其中测量这些离子对酶活性的影响。 离子例如是钠，钾，钙，镁，锰，锂，铅，锌，铜，铁或其它重金属或含有氯化物，碳酸氢盐，质子，铵的非金属离子和产生铵的物质。 所使用的酶可以是转移酶，水解酶，氧化还原酶或裂合酶。 一个基本特征是通过用结合剂掩蔽干扰离子来排除离子的干扰的方法。
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7.发明授权
公开(公告)号：US5426034A
公开(公告)日：1995-06-20
申请号：US22873
申请日：1993-02-25
CPC分类号： C12N9/18 , Y10S435/814
摘要： The invention concerns a new 6-phosphogluconolactonase which has an enzyme activity of at least 90% with respect to the initial activity after about 20 hours at pH 7.0 and 20.degree. C. In addition a process is described for the production of the enzyme from Leuconostoc. The enzyme is preferably suitable for the determination of ATP or reaction partners which can be converted into 6-phosphogluconolactone.
摘要翻译： 本发明涉及一种新的6-磷酸葡糖淀粉酶，其在pH 7.0和20℃下在约20小时后具有至少90％的初始活性的酶活性。此外，还描述了用于从明串珠菌（Leaconostoc）生产酶的方法 。 该酶优选适用于测定可以转化成6-磷酸葡糖内酯的ATP或反应伴侣。
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公开(公告)号：US5409814A
公开(公告)日：1995-04-25
申请号：US907735
申请日：1992-06-22
IPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q1/00
CPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/48 , C12Q1/527 , G01N33/64 , G01N33/84 , C12Q2334/10 , C12Q2337/12 , G01N2333/9121 , G01N2333/924 , Y10S435/962
摘要： A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.
摘要翻译： 用于测定流体中离子的方法和试剂，其中测量这些离子对酶活性的影响。 离子例如是钠，钾，钙，镁，锰，锂，铅，锌，铜，铁或其它重金属或含有氯化物，碳酸氢盐，质子，铵的非金属离子和产生铵的物质。 所使用的酶可以是转移酶，水解酶，氧化还原酶或裂合酶。 一个基本特征是通过用结合剂掩蔽干扰离子来排除离子的干扰的方法。
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