Method for producing insulin-secreting stem cells and cells derived therefrom
    1.
    发明申请
    Method for producing insulin-secreting stem cells and cells derived therefrom 审中-公开
    用于产生胰岛素分泌型干细胞的方法和由其衍生的细胞

    公开(公告)号:US20050153449A1

    公开(公告)日:2005-07-14

    申请号:US11036625

    申请日:2005-01-13

    摘要: The present invention is a method for producing an insulin-secreting pre-adipocyte stem cell and the cells derived therefrom. The preferred method comprises obtaining fat cells from a human; treating the fat cells with low-level laser energy; culturing the treated fat cells and suspending them in media together with an insulin gene plasmid; and subjecting the fat cells to transfection. The transfection is facilitated with the application of low-level laser energy.

    摘要翻译: 本发明是一种产生胰岛素分泌前脂肪细胞干细胞的方法和由其衍生的细胞。 优选的方法包括从人获得脂肪细胞; 用低水平的激光能量处理脂肪细胞; 培养经处理的脂肪细胞并与胰岛素基因质粒一起悬浮于培养基中; 并对脂肪细胞进行转染。 通过应用低级激光能量便于转染。