公开(公告)号：US5045464A

公开(公告)日：1991-09-03

申请号：US339592

申请日：1989-04-14

申请人： Susumu Ito ,  Shuji Kawai ,  Shitsuw Shikata ,  Katsuya Ozaki ,  Tadashi Yoshimatsu

发明人： Susumu Ito ,  Shuji Kawai ,  Shitsuw Shikata ,  Katsuya Ozaki ,  Tadashi Yoshimatsu

IPC分类号： C11D3/386 ,  C12N9/42 ,  C12R1/07

CPC分类号： C11D3/38645 ,  C12N9/2445 ,  C12Y302/01004 ,  C12Y302/01021

摘要： A novel alkaline cellulase is disclosed. The alkaline cellulase is produced by Bacillus sp KSM-635 (FERM BP-1485) and possesses: (1) C.sub.x enzyme activity to act on carboxymethyl cellulose and weak C.sub.1 enzyme .beta.-glucosidase activities, (2) substrate specificity to act on carboxymethyl cellulose, cellulose cotton, Avicel, filter paper, cellobiose, and p-nitrophenyl cellobioside, (3) a working pH range of 4-12 and an optimum pH range of 9-10, (4) stable pH range of 4.5-10.5 when allowed to stand at 40.degree. C. for 10 minutes, and (5) working temperature range of 10.degree.-65.degree. C. and optimum temperature range at around 40.degree. C. Its activity is not inhibited by chelating agents nor by surface active agents. It is particularly effective as an additive for laundry detergent compositions.

公开(公告)号：US4962030A

公开(公告)日：1990-10-09

申请号：US126005

申请日：1987-11-27

申请人： Shuji Kawai ,  Kazushi Oshino ,  Hiromi Okoshi ,  Hajime Mori ,  Katsuya Ozaki ,  Shitsuw Shikata ,  Susumu Ito ,  Kikuhiko Okamoto

发明人： Shuji Kawai ,  Kazushi Oshino ,  Hiromi Okoshi ,  Hajime Mori ,  Katsuya Ozaki ,  Shitsuw Shikata ,  Susumu Ito ,  Kikuhiko Okamoto

IPC分类号： C12N1/20 ,  C12N9/42

CPC分类号： C12R1/07 ,  C12N9/2437 ,  C12Y302/01004 ,  Y10S435/832

摘要： Alkaline cellulases having an optimum pH in an alkaline range and being stable over a broad pH range are produced by microorganisms which belong to the genus Bacillus and grow in a neutral medium.These alkaline cellulases are rarely inhibited by ingredients ordinarily incorporated in detergents such as surface active agents, proteinases and chelating agents, so that they can be conveniently used in detergent compositions.

摘要翻译： 在碱性范围内具有最佳pH并且在宽pH范围内稳定的碱性纤维素酶由属于芽孢杆菌属并在中性培养基中生长的微生物产生。 这些碱性纤维素酶很少被通常掺入洗涤剂如表面活性剂，蛋白酶和螯合剂中的成分抑制，因此它们可以方便地用于洗涤剂组合物中。

公开(公告)号：US4945053A

公开(公告)日：1990-07-31

申请号：US110774

申请日：1987-10-21

申请人： Susumu Ito ,  Tomokazu Sato ,  Katsuya Ozaki ,  Shitsuw Shikata ,  Kikuhiko Okamoto ,  Shigeo Inoue ,  Kenzo Koike ,  Yuichi Ota ,  Akira Takei

发明人： Susumu Ito ,  Tomokazu Sato ,  Katsuya Ozaki ,  Shitsuw Shikata ,  Kikuhiko Okamoto ,  Shigeo Inoue ,  Kenzo Koike ,  Yuichi Ota ,  Akira Takei

IPC分类号： C12N9/42

CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  Y10S435/832

摘要： Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635.These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surface active agents, chelating agents and proteinases. Therefore, they can be effectively utilized not only as an additive for clothing detergents, but also as a biomass and in other fields.

摘要翻译： 通过从Bacillus sp KSM-635的培养产物中分离得到新的碱性纤维素酶K，CMCase I和CMCase II。 这些酶稳定地在包括碱性侧面的广泛范围内工作，并且即使在低温下也显示出它们的活性。 此外，它们对表面活性剂，螯合剂和蛋白酶具有很强的抗性。 因此，它们不仅可以有效地用作衣物洗涤剂的添加剂，而且可以作为生物质和其它领域。

公开(公告)号：US06689874B2

公开(公告)日：2004-02-10

申请号：US10014436

申请日：2001-12-14

申请人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C07H2104

CPC分类号： C12N9/2457 ,  C12N9/2417 ,  C12N9/2451 ,  C12Y302/01001 ,  C12Y302/01041

摘要： The present invention provides: a nucleotide sequence encoding alkaline pullulanase and exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity; a nucleotide sequence encoding alkaline &agr;-amylase possessing an amino acid sequence described in SEQ ID NO:3; a nucleotide sequence encoding alkaline pullulanase possessing an amino acid sequence described in SEQ ID NO:4; recombinant DNAs containing these nucleotide sequences, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity.

摘要翻译： 本发明提供：编码碱基支链淀粉酶并表现出碱性α-淀粉酶和碱性支链淀粉酶活性的核苷酸序列; 编码具有SEQ ID NO：3所述的氨基酸序列的碱性α-淀粉酶的核苷酸序列; 编码具有SEQ ID NO：4所述的氨基酸序列的碱基支链淀粉酶的核苷酸序列; 含有这些核苷酸序列的重组DNA和携带重组DNA的转化微生物。 本发明的技术能够大量生产表现出碱性α-淀粉酶和碱性支链淀粉酶活性的碱性支链淀粉酶。

公开(公告)号：US06979731B1

公开(公告)日：2005-12-27

申请号：US08952741

申请日：1996-06-14

申请人： Yuji Hatada ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/28 ,  C12N15/56 ,  C12R1/07 ,  C12R1/19 ,  C07H21/04 ,  C12N15/52

CPC分类号： C12N9/2417

摘要： The present invention is directed to a liquefying alkaline alpha-amylase, and a DNA encoding for the same and functional fragments thereof.

摘要翻译： 本发明涉及液化碱性α-淀粉酶和编码其相同功能片段的DNA。

公开(公告)号：US06638748B2

公开(公告)日：2003-10-28

申请号：US09986676

申请日：2001-11-09

申请人： Yuji Hatada ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N928

CPC分类号： C12N9/2417

摘要： The present invention is directed to a liquefying alkaline alpha-amylase, and a DNA encoding for the same and functional fragments thereof.

摘要翻译： 本发明涉及液化碱性α-淀粉酶和编码其相同功能片段的DNA。

公开(公告)号：US20080050807A1

公开(公告)日：2008-02-28

申请号：US10829331

申请日：2004-04-22

申请人： Yuji Hatada ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N15/00 ,  C07H21/04

CPC分类号： C12N9/2417

摘要： The present invention provides a DNA fragment encoding alkaline liquefying α-amylase, recombinant DNA containing the DNA fragment, a transformed microorganism harboring the recombinant DNA, as well as a method for producing alkaline liquefying α-amylase using the transformant. The method of the present invention enables mass production of alkaline liquefying α-amylase useful as a detergent component.The present invention is directed to a liquefying alkaline alphα-amylase, and a DNA encoding for the same and functional fragments thereof.

摘要翻译： 本发明提供了编码碱液化α-淀粉酶的DNA片段，含有DNA片段的重组DNA，携带重组DNA的转化微生物以及使用该转化体产生碱液化α-淀粉酶的方法。 本发明的方法能够大量生产用作洗涤剂组分的碱性液化α-淀粉酶。 本发明涉及液化碱性α-淀粉酶和编码其相同功能片段的DNA。

公开(公告)号：US06338959B1

公开(公告)日：2002-01-15

申请号：US09514302

申请日：2000-02-28

申请人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N928

CPC分类号： C12N9/2457 ,  C12N9/2417 ,  C12N9/2451 ,  C12Y302/01001 ,  C12Y302/01041

摘要： The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline &agr;-amylase activity, alkaline &agr;-amylase possessing both an amino acid sequence described in SEQ ID NO:3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in SEQ ID NO:4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline &agr;-amylase activity.

摘要翻译： 本发明提供了编码具有碱性α-淀粉酶活性的碱性支链淀粉酶的DNA片段，具有SEQ ID NO：3所示的氨基酸序列的碱性α-淀粉酶和编码具有氨基酸序列的淀粉酶，碱基支链淀粉酶的DNA片段 和编码支链淀粉酶的DNA片段，含有这些DNA片段的重组DNA和含有重组DNA的转化微生物。 本发明的技术能够大量生产显示碱性α-淀粉酶活性的碱性支链淀粉酶。

公开(公告)号：US06486113B1

公开(公告)日：2002-11-26

申请号：US09381687

申请日：1999-09-23

申请人： Yuji Hatada ,  Kaori Ikawa ,  Susumu Ito ,  Kazuaki Igarashi ,  Hiroshi Hagihara ,  Yasuhiro Hayashi ,  Hiroyuki Araki ,  Katsuya Ozaki

发明人： Yuji Hatada ,  Kaori Ikawa ,  Susumu Ito ,  Kazuaki Igarashi ,  Hiroshi Hagihara ,  Yasuhiro Hayashi ,  Hiroyuki Araki ,  Katsuya Ozaki

IPC分类号： C11D3386

CPC分类号： C12N9/2417 ,  C11D3/386

摘要： The invention relates to a mutant &agr;-amylase having an amino acid sequence obtained by making deletion or replacement by another arbitrary amino acid residue of at least a methionine residue at the 202-position or a position homologous thereto among amino acid residues set forth in SEQ ID NO:1, which constitute a liquefying alkaline &agr;-amylase, a gene thereof, and a detergent composition comprising the mutant &agr;-amylase. The mutant &agr;-amylase has the optimum pH in an alkaline range, an excellent &agr;-amylase activity, and high and lasting resistance to oxidizing agents, and is hence particularly useful as a component of detergent compositions containing a bleaching agent and an oxidizing agent.

摘要翻译： 本发明涉及一种具有氨基酸序列的突变型α-淀粉酶，该氨基酸序列是通过在SEQ ID NO：1所示的氨基酸残基之间至少含有202位的甲硫氨酸残基或其同源位置的另外任意氨基酸残基进行缺失或替换而获得的 ID NO：1，其构成液化碱性α-淀粉酶，其基因和包含突变型α-淀粉酶的洗涤剂组合物。 突变型α-淀粉酶在碱性范围内具有最佳pH，优异的α-淀粉酶活性和对氧化剂的高持久耐受性，因此特别可用作含有漂白剂和氧化剂的洗涤剂组合物的组分。

公开(公告)号：US4904599A

公开(公告)日：1990-02-27

申请号：US109510

申请日：1987-10-19

申请人： Katsuya Ozaki ,  Kazushi Oshino ,  Kenzo Koike ,  Susumu Ito ,  Kikuhiko Okamoto

发明人： Katsuya Ozaki ,  Kazushi Oshino ,  Kenzo Koike ,  Susumu Ito ,  Kikuhiko Okamoto

IPC分类号： C12N15/09 ,  C12N1/20 ,  C12N9/42 ,  C12N15/00 ,  C12N15/56 ,  C12R1/07

CPC分类号： C12N9/2437 ,  C12Y302/01004

摘要： DNA frangments containing an alkaline cellulase K gene, which comprise about 4.0, about 2.4 and about 1.9 kilo base pairs, respectively, each having a specific restruction map, recombinant plasmids containing any of these DNA fragments, and recombinant microorganisms harboring any of said plasmids.The alkaline cellulase K gene is derived from a bacterial strain which belongs to the genus Bacillus and is alkalophilic, that is, capable of revealing an optimum growth in an alkaline pH region.