公开(公告)号：US06835821B2

公开(公告)日：2004-12-28

申请号：US10310730

申请日：2002-12-05

申请人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bjorn Petersen ,  Leif Nørskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

发明人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bjorn Petersen ,  Leif Nørskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

IPC分类号： C12N1557

CPC分类号： C11D3/386 ,  C12N9/54

摘要： The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.

公开(公告)号：US06506589B1

公开(公告)日：2003-01-14

申请号：US08486846

申请日：1995-06-07

申请人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bjørn Petersen ,  Leif Nørskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

发明人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bjørn Petersen ,  Leif Nørskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

IPC分类号： C12N956

CPC分类号： C11D3/386 ,  C12N9/54

摘要： The present invention relates to mutations of the subtilisin gene, some of which result in changes in the chemical characteristics of subtilisin enzyme. Mutations are created at specific nucleic acids of the subtilisin gene and, in various specific embodiments, the mutant enzymes possess altered chemical properties including, but not limited to, increased stability to oxidation, augmented proteolytic activity, and improved washability. The present invention also relates, but is not limited to, the amino acid and DNA sequences for two proteases derived from Bacillus lentus variants, subtilisin 147 and subtilisin 309, as well as mutations of these genes and the corresponding mutant enzymes.

摘要翻译： 本发明涉及枯草杆菌蛋白酶基因的突变，其中一些导致枯草杆菌蛋白酶的化学特性的变化。 在枯草杆菌蛋白酶基因的特定核酸上产生突变，并且在各种具体实施方案中，突变酶具有改变的化学性质，包括但不限于氧化增强的稳定性，增强的蛋白水解活性和改善的可洗性。 本发明还涉及但不限于来自迟缓芽孢杆菌变体，枯草杆菌蛋白酶147和枯草杆菌蛋白酶309的两种蛋白酶的氨基酸和DNA序列，以及这些基因和相应的突变酶的突变。

公开(公告)号：US06808913B2

公开(公告)日：2004-10-26

申请号：US10306089

申请日：2002-11-27

申请人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bjørn Petersen ,  Leif Nørskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

发明人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bjørn Petersen ,  Leif Nørskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

IPC分类号： C12N954

CPC分类号： C11D3/386 ,  C12N9/54

摘要： The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.

摘要翻译： 本发明涉及导致枯草杆菌蛋白酶的化学特性变化的枯草杆菌蛋白酶基因的突变。 枯草杆菌蛋白酶基因的特异性核酸的突变导致氨基酸取代，从而导致酶功能的改变。 这些突变体酶中的一些显示出对于工业应用有利的物理性质，特别是在洗涤剂工业中，提供对氧化更稳定的枯草杆菌蛋白酶，具有更大的蛋白酶活性，并且表现出改进的可洗性。

公开(公告)号：US06908991B2

公开(公告)日：2005-06-21

申请号：US10313853

申请日：2002-12-06

申请人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bjorn Petersen ,  Leif Norskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

发明人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bjorn Petersen ,  Leif Norskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

IPC分类号： C12N15/09 ,  C11D3/386 ,  C11D7/42 ,  C12N1/21 ,  C12N9/50 ,  C12N9/54 ,  C12N9/56 ,  C12N15/00 ,  C12N15/57 ,  C12P21/02 ,  C12R1/07 ,  C12R1/125 ,  C12N15/75

CPC分类号： C11D3/386 ,  C12N9/54

摘要： The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.

摘要翻译： 本发明涉及导致枯草杆菌蛋白酶的化学特性变化的枯草杆菌蛋白酶基因的突变。 枯草杆菌蛋白酶基因的特异性核酸的突变导致氨基酸取代，从而导致酶功能的改变。 这些突变体酶中的一些显示出对于工业应用有利的物理性质，特别是在洗涤剂工业中，提供对氧化更稳定的枯草杆菌蛋白酶，具有更大的蛋白酶活性，并且表现出改进的可洗性。

公开(公告)号：US5741694A

公开(公告)日：1998-04-21

申请号：US486415

申请日：1995-06-07

申请人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bj.o slashed.rn Petersen ,  Leif N.o slashed.rskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

发明人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Bj.o slashed.rn Petersen ,  Leif N.o slashed.rskov-Lauridsen ,  Villy Johannes Jensen ,  Dorrit Aaslyng

IPC分类号： C12N15/09 ,  C11D3/386 ,  C11D7/42 ,  C12N1/21 ,  C12N9/50 ,  C12N9/54 ,  C12N9/56 ,  C12N15/00 ,  C12N15/57 ,  C12P21/02 ,  C12R1/07 ,  C12R1/125

CPC分类号： C11D3/386 ,  C12N9/54

摘要： The present invention relates to mutations of the subtilisin gene, some of which result in changes in the chemical characteristics of subtilisin enzyme. Mutations are created at specific nucleic acids of the subtilisin gene and, in various specific embodiments, the mutant enzymes possess altered chemical properties including, but not limited to, increased stability to oxidation, augmented proteolytic activity, and improved washability. The present invention also relates, but is not limited to, the amino acid and DNA sequences for two proteases derived from Bacillus lentus variants, subtilisin 147 and subtilisin 309, as well as mutations of these genes and the corresponding mutant enzymes.

公开(公告)号：US20050003986A1

公开(公告)日：2005-01-06

申请号：US10896177

申请日：2004-07-21

申请人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Petersen ,  Leif Norskov-Lauridsen ,  Villy Jensen ,  Dorrit Aaslyng

发明人： Sven Hastrup ,  Sven Branner ,  Fanny Norris ,  Steffen Petersen ,  Leif Norskov-Lauridsen ,  Villy Jensen ,  Dorrit Aaslyng

IPC分类号： C12N15/09 ,  C11D3/386 ,  C11D7/42 ,  C12N1/21 ,  C12N9/50 ,  C12N9/54 ,  C12N9/56 ,  C12N15/00 ,  C12N15/57 ,  C12P21/02 ,  C12R1/07 ,  C12R1/125 ,  C07H21/04 ,  C12N15/74 ,  C12Q1/68

CPC分类号： C11D3/386 ,  C12N9/54

摘要： The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.

公开(公告)号：US06632646B1

公开(公告)日：2003-10-14

申请号：US09678867

申请日：2000-10-02

申请人： Dorrit Aaslyng ,  Sven Branner ,  Sven Hastrup ,  Leif Nørskov-Lauritsen ,  Ole Hvilsted Olsen ,  Merete Simonsen ,  Eric Casteleijn ,  Maarten Robert Egmond ,  Johan Haverkamp ,  John David Marugg ,  Arnoldus Theodorus Anthonius Mooren

发明人： Dorrit Aaslyng ,  Sven Branner ,  Sven Hastrup ,  Leif Nørskov-Lauritsen ,  Ole Hvilsted Olsen ,  Merete Simonsen ,  Eric Casteleijn ,  Maarten Robert Egmond ,  Johan Haverkamp ,  John David Marugg ,  Arnoldus Theodorus Anthonius Mooren

IPC分类号： C12N950

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38636

摘要： Enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.

摘要翻译： 提出了通过突变多个枯草杆菌蛋白酶的基因并在合适的宿主中表达突变的基因产生的酶。与其野生型亲本酶相比，酶显示改善的洗涤性能。酶是良好的 - 用于洗涤剂组合物。

公开(公告)号：US07189553B2

公开(公告)日：2007-03-13

申请号：US10314191

申请日：2002-12-06

申请人： Dorrit Aaslyng ,  Sven Branner ,  Sven Hastrup ,  Leif Norskov-Lauritsen ,  Ole Hvilsted Olsen ,  Merete Simonsen ,  Eric Castelijn ,  Maarten Robert Egmond ,  Johan Haverkamp ,  John David Marugg ,  Arnoldus Theodorus Anthonius Mooren

发明人： Dorrit Aaslyng ,  Sven Branner ,  Sven Hastrup ,  Leif Norskov-Lauritsen ,  Ole Hvilsted Olsen ,  Merete Simonsen ,  Eric Castelijn ,  Maarten Robert Egmond ,  Johan Haverkamp ,  John David Marugg ,  Arnoldus Theodorus Anthonius Mooren

IPC分类号： C12N9/54 ,  C12N15/57 ,  C12N15/74 ,  D11D3/386

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38636

摘要： The present invention relates to enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.

摘要翻译： 本发明涉及通过突变多种枯草杆菌蛋白酶的基因并在合适的宿主中表达突变的基因而产生的酶。 与其野生型亲本酶相比，酶显示改善的洗涤性能。 酶非常适合用于洗涤剂组合物中。

公开(公告)号：US06197567B1

公开(公告)日：2001-03-06

申请号：US08815775

申请日：1997-03-12

申请人： Dorrit Aaslyng ,  Sven Branner ,  Sven Hastrup ,  Leif Nørskov-Lauritsen ,  Ole Hvilsted Olsen ,  Merete Simonsen ,  Eric Casteleijn ,  Maarten Robert Egmond ,  Johan Haverkamp ,  John David Marugg ,  Arnoldus Theodorus Anthonius Mooren

发明人： Dorrit Aaslyng ,  Sven Branner ,  Sven Hastrup ,  Leif Nørskov-Lauritsen ,  Ole Hvilsted Olsen ,  Merete Simonsen ,  Eric Casteleijn ,  Maarten Robert Egmond ,  Johan Haverkamp ,  John David Marugg ,  Arnoldus Theodorus Anthonius Mooren

IPC分类号： C12N954

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38636

摘要： Enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzyme exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.

摘要翻译： 提出了通过突变多个枯草杆菌蛋白酶的基因并在合适的宿主中表达突变的基因产生的酶。与其野生型亲本酶相比，酶显示出改进的洗涤性能。酶非常适合用于洗涤剂组合物中。

公开(公告)号：US5665587A

公开(公告)日：1997-09-09

申请号：US484190

申请日：1995-06-07

申请人： Dorrit Aaslyng ,  Sven Branner ,  Sven Hastrup ,  Leif N.o slashed.rskov-Lauritsen ,  Ole Hvilsted Olsen ,  Merete Simonsen ,  Eric Casteleijn ,  Maarten Robert Egmond ,  Johan Haverkamp ,  John David Marugg ,  Arnoldus Theodorus Anthonius Mooren

发明人： Dorrit Aaslyng ,  Sven Branner ,  Sven Hastrup ,  Leif N.o slashed.rskov-Lauritsen ,  Ole Hvilsted Olsen ,  Merete Simonsen ,  Eric Casteleijn ,  Maarten Robert Egmond ,  Johan Haverkamp ,  John David Marugg ,  Arnoldus Theodorus Anthonius Mooren

IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/54 ,  C12N15/54 ,  C12N15/57 ,  C12N9/52 ,  C12N9/56

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38636

摘要： The present invention relates to modified subtilisins comprising a mutation in an amino acid sequence of a subtilisin at a position selected from the group consisting of 1, 2, 3, 4, 6, 9, 10, 12, 14, 15, 17, 18, 19, 20, 21, 22, 24, 25, 27, 36, 37, 38, 40, 41, 43, 44, 45, 46, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 75, 76, 77, 78, 79, 87, 89, 91, 94, 97, 98, 99, 100, 101, 103, 104, 105, 106, 107, 108, 109, 112, 113, 115, 116, 117, 118, 120, 126, 128, 129, 130, 131, 133, 134, 136, 137, 140, 141, 143, 144, 145, 146, 155, 156, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 170, 171, 172, 173, 181, 182, 183, 184, 185, 186, 188, 189, 191, 192, 194, 195, 197, 204, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 259, 260, 261, 262, 263, 265, 269, 271, 272 and 275. The present invention also relates to detergent compositions comprising a modified subtilisin of the present invention.

摘要翻译： 本发明涉及包含在选自1,2,3,4,6,9,10,12,14,15,17,18中的位置的枯草杆菌蛋白酶的氨基酸序列突变的修饰的枯草杆菌蛋白酶 ，19，20，21，22，24，25，27，36，37，38，40，41，43，44，45，46，49，50，51，52，53，54，55，56，57 ，58，59，60，61，62，75，76，77，78，79，87，89，91，94，97，98，99，100，101，103，104，105，106，107，108 ，109,112,113,115,116,117,118,120,126,128,129,130​​,131,133,134,136,137,140,​​141,143,144,145,146,155,156 ，158,159,160,161,162,163,164,165,166,167,170,171,172,173,181,182,183,184,185,186,188,189,191,192,194 ，195,197,204,206,29,210,211,212,213,214,215,216,217,218,235,236,237,238,239,240,241,242,243,244,245 ，247,248,249,251,252,253,254,255,256,257,259,260,261,262,263,265,269,271,272和275.本发明还涉及洗涤剂组合物，其包含 p的修饰的枯草杆菌蛋白酶 重申发明。