公开(公告)号：US06652853B2

公开(公告)日：2003-11-25

申请号：US09800522

申请日：2001-03-08

申请人： Sydney Welt ,  Nancy Kemeny ,  Gerd Ritter ,  Achim A. Jungbluth ,  Leonard Cohen ,  Lloyd J. Old

发明人： Sydney Welt ,  Nancy Kemeny ,  Gerd Ritter ,  Achim A. Jungbluth ,  Leonard Cohen ,  Lloyd J. Old

IPC分类号： A61K39395

CPC分类号： A61K39/395 ,  A61K39/39558 ,  A61K45/06 ,  A61K2039/505 ,  C07K16/3046 ,  C07K2317/24 ,  A61K31/70 ,  A61K31/555 ,  A61K31/52 ,  A61K31/505 ,  A61K31/475 ,  A61K31/47 ,  A61K31/17 ,  A61K2300/00

摘要： This invention relates to a combination of immunotherapy and chemotherapy to promote tumor regression by treating a patient in need thereof with a combination of a humanized antibody that binds to A33 antigen and one or more chemotherapeutic agents. The method is useful for treating patients with colorectal cancer and gastric carcinomas. The method is particularly useful for treating patients who have tumors that are resistant to one or more chemotherapeutic agents and/or have metastasized.

公开(公告)号：US5851526A

公开(公告)日：1998-12-22

申请号：US449911

申请日：1995-05-25

申请人： Sydney Welt ,  Gerd Ritter ,  Leonard Cohen ,  Clarence Williams, Jr. ,  Elizabeth Carswell Richards ,  Mary John ,  Lloyd J. Old

发明人： Sydney Welt ,  Gerd Ritter ,  Leonard Cohen ,  Clarence Williams, Jr. ,  Elizabeth Carswell Richards ,  Mary John ,  Lloyd J. Old

IPC分类号： C12N15/02 ,  A61K31/17 ,  A61K31/475 ,  A61K31/505 ,  A61K38/00 ,  A61K39/395 ,  A61K47/48 ,  A61K51/00 ,  A61K51/10 ,  A61P1/00 ,  A61P35/00 ,  C07K16/30 ,  C12N5/10 ,  C12P21/08 ,  C12R1/91 ,  A61K39/00

CPC分类号： A61K51/1063 ,  A61K47/48615 ,  C07K16/3046 ,  A61K2039/505 ,  A61K2121/00 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/77 ,  C07K2319/00

摘要： This invention relates to methods of reducing the effects of colon cancer tumors. Various agents are conjugated to monoclonal antibodies which are specific for colon cancer cells. The conjugates are administered to patients having colon cancer such that the effects of the cancer are reduced.

公开(公告)号：US06190640B1

公开(公告)日：2001-02-20

申请号：US08752693

申请日：1996-11-19

申请人： Sydney Welt ,  Gerd Ritter ,  Leonard Cohen ,  Clarence Williams, Jr. ,  Elizabeth Carswell Richards ,  Mary John ,  Lloyd J. Old

发明人： Sydney Welt ,  Gerd Ritter ,  Leonard Cohen ,  Clarence Williams, Jr. ,  Elizabeth Carswell Richards ,  Mary John ,  Lloyd J. Old

IPC分类号： A61K5100

CPC分类号： C07K16/3046 ,  A61K38/00 ,  A61K47/6863 ,  A61K51/1063 ,  A61K2039/505 ,  A61K2121/00 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/77 ,  C07K2319/00

摘要： The invention involves the therapeutic use of humanized antibodies, fragments, etc., which bind to the antigen A33. These antibody molecules are useful in the treatment of various neoplasias, including colon cancer. Various modes of administration, and doses are described.

摘要翻译： 本发明涉及结合抗原A33的人源化抗体，片段等的治疗用途。 这些抗体分子可用于治疗各种肿瘤，包括结肠癌。 描述了各种给药方式和剂量。

公开(公告)号：US06342587B1

公开(公告)日：2002-01-29

申请号：US09425638

申请日：1999-10-22

申请人： Carlos F. Barbas, III ,  Christoph Rader ,  Gerd Ritter ,  Sydney Welt ,  Lloyd J. Old

发明人： Carlos F. Barbas, III ,  Christoph Rader ,  Gerd Ritter ,  Sydney Welt ,  Lloyd J. Old

IPC分类号： C07K1628

CPC分类号： C07K16/3046 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/565

摘要： The invention is directed to novel CDRs and immunoglobulin products that bind to A33 antigens and methods for their use. The invention also involves a method for making humanized antibodies, using a rabbit as a host animal, and phage display library methodologies, and the antibodies themselves. The methodology is useful, for example, in generating humanized antibodies against molecules associated with cancer, such as A33, which is associated with colon cancer.

摘要翻译： 本发明涉及结合A33抗原的新型CDR和免疫球蛋白产物及其使用方法。 本发明还涉及使用兔作为宿主动物制造人源化抗体的方法，以及噬菌体展示文库方法以及抗体本身。 该方法可用于例如产生针对与癌症相关的分子的人源化抗体，例如与结肠癌相关的A33。

公开(公告)号：US06346249B1

公开(公告)日：2002-02-12

申请号：US09543004

申请日：2000-04-04

申请人： Carlos F. Barbas, III ,  Christoph Rader ,  Gerd Ritter ,  Sydney Welt ,  Lloyd J. Old

发明人： Carlos F. Barbas, III ,  Christoph Rader ,  Gerd Ritter ,  Sydney Welt ,  Lloyd J. Old

IPC分类号： A61K39395

CPC分类号： C07K16/3046 ,  A61K2039/505 ,  C07K16/00 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/565

摘要： The invention is directed to methods of reducing the effects of cancer in a subject by administering to said subject a pharmaceutically effective amount of an anti-cancer agent conjugated to an inmmunoglobulin product which comprises one or more novel complementarity determining region and framework regions.

摘要翻译： 本发明涉及通过向所述受试者施用药学有效量的与包含一个或多个新的互补决定区和框架区的免疫球蛋白产物缀合的抗癌剂来减少受试者中癌症的影响的方法。

公开(公告)号：US5712369A

公开(公告)日：1998-01-27

申请号：US597495

申请日：1996-02-02

申请人： Lloyd J. Old ,  Sydney Welt ,  Gerd Ritter ,  Richard J. Simpson ,  Edouard Nice ,  R. L. Moritz ,  B. Catimel ,  Hong Ji ,  Anthony W. Burgess ,  Joan K. Heath ,  Sara J. White ,  Cameron Johnstone

发明人： Lloyd J. Old ,  Sydney Welt ,  Gerd Ritter ,  Richard J. Simpson ,  Edouard Nice ,  R. L. Moritz ,  B. Catimel ,  Hong Ji ,  Anthony W. Burgess ,  Joan K. Heath ,  Sara J. White ,  Cameron Johnstone

IPC分类号： C07K14/47 ,  C07K14/705 ,  C07K16/30 ,  C07K14/00 ,  A61K38/00 ,  A61K39/00 ,  A61K39/38

CPC分类号： C07K16/3046 ,  C07K14/47 ,  C07K14/705

摘要： This invention relates to isolated protein and to peptides which are found on the surface of colon cells and colon cancer cells, as well as to nucleic acid molecules encoding said protein and peptides. The protein and peptides bind to tumor-associated antibodies, such as mAb A33. The monomeric protein has a molecular weight of about 43 kD as determined by SDS gel electrophoresis under non-reducing conditions. In addition, this invention relates to the use of said nucleic acid molecules, protein, in monomeric or multimeric form, and to antibodies to said peptides in diagnostic, screening and therapeutic methods. This invention further relates to antibodies specific for said protein, in monomeric or multimeric form, and to antibodies to said peptides.

摘要翻译： 本发明涉及分离的蛋白质和在结肠细胞和结肠癌细胞的表面上发现的肽，以及编码所述蛋白质和肽的核酸分子。 蛋白质和肽结合肿瘤相关抗体，如mAb A33。 单体蛋白质在非还原条件下通过SDS凝胶电泳测定的分子量为约43kD。 此外，本发明涉及在诊断，筛选和治疗方法中所述核酸分子，蛋白质，单体或多聚体形式以及所述肽的抗体的用途。 本发明还涉及以单体或多聚体形式对所述蛋白质特异性的抗体以及针对所述肽的抗体。

公开(公告)号：US06518415B1

公开(公告)日：2003-02-11

申请号：US08859648

申请日：1997-05-20

申请人： Kathryn Lesley Armour ,  Francis Joseph Carr ,  Lloyd J. Old ,  Elisabeth Stockert ,  Sydney Welt ,  Kunio Kitamura ,  Pilar Garin-Chesa

发明人： Kathryn Lesley Armour ,  Francis Joseph Carr ,  Lloyd J. Old ,  Elisabeth Stockert ,  Sydney Welt ,  Kunio Kitamura ,  Pilar Garin-Chesa

IPC分类号： C07H2104

CPC分类号： C07K16/34 ,  C07K16/30 ,  C07K2317/24 ,  C07K2319/00

摘要： The invention provides for the production of several humanized murine antibodies specific for the antigen Lewis Y, which is recognized by the murine antibody Lewis Y. The Lewis Y antigen is expressed in normal tissues but the level of expression is higher in certain tumor types so that the antigen can be used as a marker for cells of some breast, colon, gastric, esophageal, pancreatic, duodenal, lung, bladder and renal carcinomas and gastric and islet cell neuroendocrine tumors. The invention also provides for numerous polynucleotide encoding humanized Lewis Y specific antibodies, expression vectors for producing humanized Lewis Y specific antibodies, and host cells for the recombinant production of the humanized antibodies. The invention also provides methods for detecting cancerous cells (in vitro and in vivo) using humanized Lewis Y specific antibodies. Additionally, the invention provides methods of treating cancer using humanized Lewis Y specific antibodies.

摘要翻译： 本发明提供对抗原Lewis Y特异性的几种人源化鼠抗体的产生，其被鼠抗体Lewis Y识别。Lewis Y抗原在正常组织中表达，但在某些肿瘤类型中表达水平较高，因此 抗原可用作乳腺癌，结肠癌，胃癌，食管癌，胰腺癌，十二指肠癌，肺癌，膀胱癌和肾癌和胃和胰岛细胞神经内分泌肿瘤的细胞标志物。 本发明还提供编码人源化Lewis Y特异性抗体的许多多核苷酸，用于产生人源化Lewis Y特异性抗体的表达载体和用于重组产生人源化抗体的宿主细胞。 本发明还提供使用人源化Lewis Y特异性抗体检测癌细胞（体外和体内）的方法。 另外，本发明提供使用人源化Lewis Y特异性抗体治疗癌症的方法。

公开(公告)号：US5874060A

公开(公告)日：1999-02-23

申请号：US859649

申请日：1997-05-20

申请人： Kathryn Lesley Armour ,  Francis Joseph Carr ,  Lloyd J. Old ,  Elisabeth Stockert ,  Sydney Welt ,  Kunio Kitamura ,  Pilar Garin-Chesa

发明人： Kathryn Lesley Armour ,  Francis Joseph Carr ,  Lloyd J. Old ,  Elisabeth Stockert ,  Sydney Welt ,  Kunio Kitamura ,  Pilar Garin-Chesa

IPC分类号： C07K16/30 ,  C07K16/34 ,  A61K51/00 ,  A61K39/395

CPC分类号： C07K16/34 ,  C07K16/30 ,  C07K2317/24 ,  C07K2319/00

摘要： The invention provides for the production of several humanized murine antibodies specific for the antigen Lewis Y, which is recognized by the murine antibody Lewis Y. The Lewis Y antigen is expressed in normal tissues but the level of expression is higher in certain tumor types so that the antigen can be used as a marker for cells of some breast, colon, gastric, esophageal, pancreatic, duodenal, lung, bladder and renal carcinomas and gastric and islet cell neuroendocrine tumors. The invention also provides for numerous polynucleotide encoding humanized Lewis Y specific antibodies, expression vectors for producing humanized Lewis Y specific antibodies, and host cells for the recombinant production of the humanized antibodies. The invention also provides methods for detecting cancerous cells (in vitro and in vivo) using humanized Lewis Y specific antibodies. Additionally, the invention provides methods of treating cancer using humanized Lewis Y specific antibodies.

公开(公告)号：US5160723A

公开(公告)日：1992-11-03

申请号：US671132

申请日：1991-03-18

申请人： Sydney Welt ,  Chaitanya R. Divgi ,  Samual Yeh ,  Lloyd J. Old ,  Herbert F. Oettgen ,  Francisco X. Real ,  Junichi Sakamoto

发明人： Sydney Welt ,  Chaitanya R. Divgi ,  Samual Yeh ,  Lloyd J. Old ,  Herbert F. Oettgen ,  Francisco X. Real ,  Junichi Sakamoto

IPC分类号： A61K51/10 ,  C07K16/30

CPC分类号： A61K51/1063 ,  C07K16/3046 ,  A61K2039/505 ,  A61K2121/00 ,  C07K2317/24 ,  C07K2317/55 ,  Y10S436/808 ,  Y10S436/813 ,  Y10S530/808 ,  Y10S530/809 ,  Y10S530/866

摘要： This invention provides a method of imaging a colorectal carcinoma lesion in a human patient which comprises administering to the patient a monoclonal antibody capable of binding to a cell surface antigen associated with the coloretal carcinoma lesion and which is labeled with an imaging agent under conditions so as to form a complex between the monoclonal antibody and the cell surface antigen, imaging any complex so formed, and thereby imaging the colorectal carcinoma lesion.This invention also provides a monoclonal antibody designated AS 33 (ATCC Accession No. HB 8779) and the hybridoma which produces it.

摘要翻译： 本发明提供了一种在人类患者中对结肠直肠癌病变进行成像的方法，其包括向患者施用能够结合与该结肠癌病变相关的细胞表面抗原的单克隆抗体，并在成像剂的条件下进行标记， 在单克隆抗体和细胞表面抗原之间形成复合物，对如此形成的任何复合物进行成像，从而对结肠直肠癌病变进行成像。 本发明还提供了称为AS33（ATCC登录号HB 8779）和产生它的杂交瘤的单克隆抗体。

公开(公告)号：US5958412A

公开(公告)日：1999-09-28

申请号：US869102

申请日：1997-06-04

申请人： Sydney Welt ,  Lloyd J. Old ,  Elsje Barendswaard ,  Nicholas J. Montalto ,  Ali Osmay Gure

发明人： Sydney Welt ,  Lloyd J. Old ,  Elsje Barendswaard ,  Nicholas J. Montalto ,  Ali Osmay Gure

IPC分类号： C12N15/02 ,  A61K31/17 ,  A61K31/475 ,  A61K31/505 ,  A61K38/00 ,  A61K39/395 ,  A61K47/48 ,  A61K51/00 ,  A61K51/10 ,  A61P1/00 ,  A61P35/00 ,  C07K16/30 ,  C12N5/10 ,  C12P21/08 ,  C12R1/91

CPC分类号： A61K51/1063 ,  A61K47/48615 ,  C07K16/3046 ,  A61K2039/505 ,  A61K2121/00 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/77 ,  C07K2319/00

摘要： This invention relates to methods of reducing the effects of colon cancer tumors. Various agents are conjugated to monoclonal antibodies which are specific for colon cancer cells. The conjugates are administered to patients having colon cancer such that the effects of the cancer are reduced.

摘要翻译： 本发明涉及减少结肠癌肿瘤的作用的方法。 各种试剂与对结肠癌细胞特异性的单克隆抗体缀合。 将结合物施用于具有结肠癌的患者，使得癌症的作用降低。