公开(公告)号：US11148138B2

公开(公告)日：2021-10-19

申请号：US15756176

申请日：2015-09-02

Applicant: TECAN TRADING AG ,  Thomas Perroud ,  Dan Tran ,  Daniel Hoffmeyer ,  Tiffany Lay ,  Manjeet Dhindsa

Inventor： Thomas Perroud ,  Dan Tran ,  Daniel Hoffmeyer ,  Tiffany Lay ,  Manjeet Dhindsa

IPC: B01L3/00

Abstract: A digital microfluidics system with electrodes attached to a substrate and covered by a hydrophobic surface, and a control unit for manipulating liquid droplets by electrowetting; providing in close proximity to electrodes a magnetic conduit for directing a magnetic field of a backing magnet to the first hydrophobic surface; providing on the hydrophobic surface a liquid droplet that has magnetically responsive beads; moving by electrowetting the liquid droplet with the magnetically responsive beads until a part of which is placed atop of the magnetic conduit; actuating the backing magnet of the magnetic conduit and attracting/concentrating magnetically responsive beads; and while actuating the backing magnet, moving by electrowetting the liquid droplet with decreased number of magnetically responsive beads away from the specific magnetic conduit. Also disclosed are a method for suspending magnetically responsive beads in liquid portions or droplets in digital microfluidics and a disposable cartridge to carry out the methods.

公开(公告)号：US09784711B2

公开(公告)日：2017-10-10

申请号：US14402717

申请日：2014-01-06

Applicant: TECAN TRADING AG

Inventor： Daniel Hoffmeyer ,  Tiffany Lay ,  Travis Lee ,  Marc N. Feiglin ,  Werner Halg ,  Anne R. Kopf-Sill

IPC: G01N27/447 ,  B01L3/00 ,  B81B1/00

CPC classification number: G01N27/44791 ,  B01L3/502715 ,  B01L3/502792 ,  B01L3/505 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2300/044 ,  B01L2300/123 ,  B01L2400/0427 ,  B81B1/00 ,  G01N27/44756

Abstract: A disposable cartridge used in a digital microfluidics system has a bottom layer with first hydrophobic surface, a rigid cover plate with second hydrophobic surface, and a gap there-between. The bottom layer is a flexible film on an uppermost surface of a cartridge accommodation site of a system, attracted to and spread over the uppermost surface by an underpressure. A lower surface of the plate and the flexible bottom layer are sealed to each other. The assembled cartridge is removed from the cartridge accommodation site in one piece and potentially includes samples and processing fluids. The system has a base unit and a cartridge accommodation site with an electrode array of individual electrodes and a central control unit for controlling selection of individual electrodes and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the gap by electrowetting.

公开(公告)号：US11819845B2

公开(公告)日：2023-11-21

申请号：US17469656

申请日：2021-09-08

Applicant: TECAN TRADING AG

Inventor： Thomas Perroud ,  Dan Tran ,  Daniel Hoffmeyer ,  Tiffany Lay ,  Manjeet Dhindsa

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/508 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/025 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0427

Abstract: A digital microfluidics system with electrodes attached to a substrate and covered by a hydrophobic surface and a control unit for manipulating liquid droplets by electrowetting, providing in close proximity to electrodes a magnetic conduit for directing a magnetic field of a backing magnet to the first hydrophobic surface, providing on the hydrophobic surface a liquid droplet that has magnetically responsive beads moving by electrowetting the liquid droplet with the magnetically responsive beads until a part of which is placed atop of the magnetic conduit, actuating the backing magnet of the magnetic conduit and attracting/concentrating magnetically responsive beads while actuating the backing magnet, moving by electrowetting the liquid droplet with decreased number of magnetically responsive beads away from the specific magnetic conduit. Also disclosed are a method for suspending magnetically responsive beads in liquid portions or droplets in digital microfluidics and a disposable cartridge to carry out the methods.

公开(公告)号：US09377439B2

公开(公告)日：2016-06-28

申请号：US13737656

申请日：2013-01-09

Applicant: TECAN TRADING AG

Inventor： Travis Lee ,  Tiffany Lay ,  Bruce Richardson ,  S. Nicholas Reimitz

IPC: G01N27/447 ,  B01L3/00

CPC classification number: G01N27/44791 ,  B01L3/502715 ,  B01L3/502792 ,  B01L3/505 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/043 ,  B01L2300/044 ,  B01L2300/089 ,  B01L2300/123 ,  B01L2400/0427

Abstract: A disposable cartridge had body, bottom layer with first hydrophobic surface, top layer with a second hydrophobic surface, and gap between. The bottom layer is a flexible film sealingly attached at its circumference to the top layer. No spacer is between the layers. The top layer has loading sites for transferring processing liquids into the gap. In use, the bottom layer is a working film for manipulating samples in liquid droplets and is placed on an electrode array of a digital microfluidics system having base unit with cartridge accommodation site and electrode array at the site. The electrode array is supported by a bottom substrate that extends in a first plane and has individual electrodes. The system also includes a central control unit for controlling selection of the electrodes and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the gap by electrowetting.

Abstract translation: 一次性药筒具有主体，底层具有第一疏水性表面，顶层具有第二疏水性表面，并且之间具有间隙。 底层是在其周围密封地附接到顶层的柔性膜。 层之间没有间隔物。 顶层具有用于将处理液体转移到间隙中的装载位置。 在使用中，底层是用于操作液滴中的样品的工作薄膜，并且放置在具有基座单元的数字微流体系统的电极阵列上，该基座具有筒位置和位置处的电极阵列。 电极阵列由在第一平面中延伸并具有单独电极的底部基底支撑。 该系统还包括一个中央控制单元，用于控制电极的选择，并为这些电极提供单独的电压脉冲，用于通过电润湿来操纵间隙内的液滴。

公开(公告)号：US20140353157A1

公开(公告)日：2014-12-04

申请号：US14361556

申请日：2014-01-06

Applicant: TECAN TRADING AG

Inventor： Daniel Hoffmeyer ,  Tiffany Lay ,  Travis Lee

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  B01L3/502715 ,  B01L3/502792 ,  B01L3/505 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2300/044 ,  B01L2300/123 ,  B01L2400/0427 ,  B81B1/00 ,  G01N27/44756

Abstract: A digital microfluidics system for manipulating samples in liquid droplets within a gap between a first hydrophobic surface of a bottom layer and a second hydrophobic surface of at least one disposable cartridge. Disposable cartridges comprise a body and/or a rigid cover plate. The bottom layer of each disposable cartridge is a flexible film that is sealingly attached to the body or plate. The cartridge has no spacer between the first and second hydrophobic surfaces. When using these cartridges, the bottom layers configured as a working film for manipulating samples in liquid droplets thereon, is placed on an electrode array of a digital microfluidics system. The array has individual electrodes. The digital microfluidics system also comprises a central control unit for controlling the selection of the individual electrodes of the electrode array and for providing these electrodes with individual voltage pulses for manipulating liquid droplets by electrowetting.

Abstract translation: 一种数字微流体系统，用于在底层的第一疏水表面和至少一个一次性盒的第二疏水表面之间的间隙内操作液滴中的样品。 一次性盒包括主体和/或刚性盖板。 每个一次性盒的底层是密封地附接到主体或板上的柔性膜。 墨盒在第一和第二疏水表面之间没有间隔物。 当使用这些盒时，将配置为工作膜的底层用于操作其上液滴的样品放置在数字微流体系统的电极阵列上。 该阵列具有单独的电极。 数字微流体系统还包括一个中央控制单元，用于控制电极阵列的各个电极的选择，并为这些电极提供单独的电压脉冲，用于通过电润湿来操纵液滴。

公开(公告)号：US10137450B2

公开(公告)日：2018-11-27

申请号：US14335027

申请日：2014-07-18

Applicant: Tecan Trading AG

Inventor： Tiffany Lay ,  Daniel Hoffmeyer ,  Anne R. Kopf-Sill

IPC: B01L3/00 ,  B01L3/02

Abstract: A disposable cartridge configured as a digital microfluidics system for manipulating samples in liquid portions having a cartridge accommodation site and a central control unit for controlling selection of individual electrodes of an electrode array located at the site and for providing plural electrodes with individual voltage pulses for manipulating liquid portions by electrowetting. The cartridge has a hydrophobic working surface and a rigid cover with a second hydrophobic surface, the hydrophobic surfaces facing each other and being separated in parallel planes by a gap. The cartridge has plural pipetting guides for safe entering/withdrawing liquids into/from the gap with a pipette tip. At least one of the pipetting guides provides an abutting surface sealingly admittable by a counter surface of a pipette tip, located at a pipetting orifice that reaches through the rigid cover, and configured to prevent a pipette tip from touching the hydrophobic working surface.

公开(公告)号：US09915631B2

公开(公告)日：2018-03-13

申请号：US14361556

申请日：2014-01-06

Applicant: TECAN TRADING AG

Inventor： Daniel Hoffmeyer ,  Tiffany Lay ,  Travis Lee

IPC: G01N27/447 ,  B01L3/00 ,  B81B1/00

CPC classification number: G01N27/44791 ,  B01L3/502715 ,  B01L3/502792 ,  B01L3/505 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2300/044 ,  B01L2300/123 ,  B01L2400/0427 ,  B81B1/00 ,  G01N27/44756

Abstract: A digital microfluidics system for manipulating samples in liquid droplets within a gap between a first hydrophobic surface of a bottom layer and a second hydrophobic surface of at least one disposable cartridge. Disposable cartridges comprise a body and/or a rigid cover plate. The bottom layer of each disposable cartridge is a flexible film that is sealingly attached to the body or plate. The cartridge has no spacer between the first and second hydrophobic surfaces. When using these cartridges, the bottom layers configured as a working film for manipulating samples in liquid droplets thereon, is placed on an electrode array of a digital microfluidics system. The array has individual electrodes. The digital microfluidics system also comprises a central control unit for controlling the selection of the individual electrodes of the electrode array and for providing these electrodes with individual voltage pulses for manipulating liquid droplets by electrowetting.

公开(公告)号：US20140190832A1

公开(公告)日：2014-07-10

申请号：US13902384

申请日：2013-05-24

Applicant: Tecan Trading AG

Inventor： Tiffany Lay ,  Thomas D. Perroud ,  Michael Benjamin Franklin ,  Anne R. Kopf-Sill

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502792 ,  B01L3/505 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/043 ,  B01L2300/044 ,  B01L2300/089 ,  B01L2300/123 ,  B01L2400/0427

Abstract: Digital microfluidics system manipulates samples in liquid droplets within a gap of at least one disposable cartridge. It is also provides additional space for collecting and/or storing waste fluids in this digital microfluidics system. It includes at least one waste hollow which is fluidly connected with a gap of a disposable cartridge that includes a bottom layer with a first hydrophobic surface and a top layer with a second hydrophobic surface. The waste hollow is located next to at least one individual waste electrode that is positioned next to at least one individual electrode of an electrode array. Each individual waste electrode is operatively connected to a central control unit and covers in each case a waste electrode area.

Abstract translation: 数字微流体系统在至少一个一次性盒的间隙内操作液滴中的样品。 它还提供了用于在该数字微流体系统中收集和/或存储废流体的额外空间。 它包括至少一个与一次性盒的间隙流体连接的废物中空层，其包括具有第一疏水表面的底层和具有第二疏水表面的顶层。 废物中空部位于靠近电极阵列的至少一个单独电极的至少一个单独的废电极旁边。 每个单独的废电极可操作地连接到中央控制单元，并且在每种情况下覆盖废电极区域。

公开(公告)号：US10913062B2

公开(公告)日：2021-02-09

申请号：US15756209

申请日：2016-09-01

Applicant: TECAN TRADING AG

Inventor： Thomas Perroud ,  Daniel Tran ,  Daniel Hoffmeyer ,  Tiffany Lay ,  Manjeet Dhindsa

IPC: B01L3/00

Abstract: Digital microfluidics system with electrodes attached to a PCB has control unit for manipulating liquid droplets by electrowetting, cartridge accommodation site for taking up a disposable cartridge having a working gap in-between two hydrophobic surfaces, and magnetic conduit/backing combination. A barrier element on an individual electrode of the PCB for narrowing the working gap. A disposable cartridge is positioned at the cartridge accommodation site, its flexible working film touching there and of the barrier element an uppermost surface. In the working gap and above a path of selected electrodes a liquid portion or liquid droplet with magnetically responsive beads moves by electrowetting on the electrode path until a magnetic field of the magnetic conduit is reached. The backing magnet is activated before and during the moving to thereby attract and remove magnetically responsive beads therefrom.

公开(公告)号：US09630176B2

公开(公告)日：2017-04-25

申请号：US13902384

申请日：2013-05-24

Applicant: Tecan Trading AG

Inventor： Tiffany Lay ,  Thomas D. Perroud ,  Michael Benjamin Franklin ,  Anne R. Kopf-Sill

IPC: B01L3/00 ,  B01L9/00

CPC classification number: B01L3/502715 ,  B01L3/502792 ,  B01L3/505 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/043 ,  B01L2300/044 ,  B01L2300/089 ,  B01L2300/123 ,  B01L2400/0427

Abstract: Digital microfluidics system manipulates samples in liquid droplets within a gap of at least one disposable cartridge. It is also provides additional space for collecting and/or storing waste fluids in this digital microfluidics system. It includes at least one waste hollow which is fluidly connected with a gap of a disposable cartridge that includes a bottom layer with a first hydrophobic surface and a top layer with a second hydrophobic surface. The waste hollow is located next to at least one individual waste electrode that is positioned next to at least one individual electrode of an electrode array. Each individual waste electrode is operatively connected to a central control unit and covers in each case a waste electrode area.