公开(公告)号：US20170233699A1

公开(公告)日：2017-08-17

申请号：US15463003

申请日：2017-03-20

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Xiao-Dong Chen

IPC: C12N5/077 ,  A61K35/28 ,  A61K35/34 ,  C12N5/0775

CPC classification number: C12N5/0657 ,  A61K35/28 ,  A61K35/34 ,  C12N5/0605 ,  C12N5/0662 ,  C12N5/0663 ,  C12N5/0665 ,  C12N2501/06 ,  C12N2501/115 ,  C12N2502/1323 ,  C12N2533/52 ,  C12N2533/90

Abstract: In some aspects, this invention provides a method of making a bone marrow-derived tissue-specific stem cell proliferation, expansion, isolation and rejuvenation extracellular matrix. In other aspects, this invention provides a method of making a tissue-specific fibroblast-derived stem cell differentiation extracellular matrix. Also provided are methods of using such a cell-derived preservation or differentiation matrices to induce tissue-specific differentiation of pluripotent cells, repair damaged tissue, and treat a subject having a physiologic deficiency using the same.

公开(公告)号：US20210079351A1

公开(公告)日：2021-03-18

申请号：US16875142

申请日：2020-05-15

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Xiao-Dong Chen

IPC: C12N5/077 ,  C12N5/0775 ,  C12N5/073 ,  A61K35/28 ,  A61K35/34

Abstract: In some aspects, this invention provides a method of making a bone marrow-derived tissue-specific stem cell proliferation, expansion, isolation and rejuvenation extracellular matrix. In other aspects, this invention provides a method of making a tissue-specific fibroblast-derived stem cell differentiation extracellular matrix. Also provided are methods of using such a cell-derived preservation or differentiation matrices to induce tissue-specific differentiation of pluripotent cells, repair damaged tissue, and treat a subject having a physiologic deficiency using the same.

公开(公告)号：US10377986B2

公开(公告)日：2019-08-13

申请号：US15118539

申请日：2015-02-09

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Xiao-Dong Chen ,  Chih-ko Yeh ,  Joo L. Ong

IPC: C12N5/071 ,  A61L27/38 ,  A61L27/22

Abstract: A cell culture system including a silk fibroid scaffold, culture media, and salivary gland cells. The salivary gland cells grown in the tissue culture system have physiological and morphological features like those of in vivo salivary gland cells. The cell culture system can be used to produce a salivary tissue-specific extracellular matrix capable of inducing differentiation of salivary gland cell precursors into salivary gland cells.

公开(公告)号：US20170056557A1

公开(公告)日：2017-03-02

申请号：US15118539

申请日：2015-02-09

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Xiao-Dong Chen ,  Chih-ko Yeh ,  Joo L. Ong

IPC: A61L27/36 ,  A61L27/38 ,  G01N33/50 ,  C12N5/071 ,  C12N5/00

CPC classification number: C12N5/0633 ,  A61L27/227 ,  A61L27/3813 ,  C12N2501/999 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/56

Abstract: A cell culture system including a silk fibroid scaffold, culture media, and salivary gland cells. The salivary gland cells grown in the tissue culture system have physiological and morphological features like those of in vivo salivary gland cells. The cell culture system can be used to produce a salivary tissue-specific extracellular matrix capable of inducing differentiation of salivary gland cell precursors into salivary gland cells.

Abstract translation: 包括丝纤维支架，培养基和唾液腺细胞的细胞培养系统。 在组织培养系统中生长的唾液腺细胞具有生物学和形态特征，如体内唾液腺细胞。 细胞培养系统可用于产生能够诱导唾液腺细胞前体分化为唾液腺细胞的唾液组织特异性细胞外基质。