公开(公告)号：US20230173449A1

公开(公告)日：2023-06-08

申请号：US17996927

申请日：2021-04-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Joseph De Rutte ,  Dino Di Carlo ,  Sohyung Lee

IPC: B01J13/14 ,  G01N33/58

CPC classification number: B01J13/14 ,  G01N33/585

Abstract: A method of fabricating shaped particles is disclosed. The method involves generating a plurality of droplets within dispersion media (e.g., oil and surfactant), the plurality of droplets formed from a mixture of precursor materials that are in a miscible state. A stimulus or change of conditions is then introduced to the droplets so as to cause the mixture of precursor materials to become immiscible and phase-separate from one another. The phase-separated droplets are then crosslinked to form shaped particles. The stimulus or change of conditions may include one or more of the following: a change in temperature, a change in pH, a change in osmolarity, a change composition of the droplets, a change in the composition of the dispersion media. The shaped particles may be washed to remove un-crosslinked material and one or more affinity capture agents may be immobilized onto the shaped particles.

公开(公告)号：US20230257732A1

公开(公告)日：2023-08-17

申请号：US18006382

申请日：2021-08-04

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Mark van Zee ,  Dino Di Carlo ,  Joseph de Rutte ,  Sohyung Lee ,  Robert Damoiseaux ,  Cayden Williamson

IPC: C12N11/04 ,  C12N5/00 ,  C12N1/12 ,  C12N11/10

CPC classification number: C12N11/04 ,  C12N5/0012 ,  C12N5/00 ,  C12N1/12 ,  C12N11/10 ,  C12N2537/10 ,  C12N2539/10 ,  C12N2509/00 ,  C12N2533/50 ,  C12N2533/70

Abstract: Degradable hollow shell particles are disclosed that can encapsulate cells within the hollow inner cavity that allows for the high-throughput screening and sorting of the encapsulated cells based on their phenotypic properties. The solid-phase of the particle is porous such that solution exchange can occur between the external environment and the interior cavity. Further, the solid-phase contains degradable crosslinkers and can be degraded to release enclosed biological entities. An example embodiment consists of encapsulating a cell within the hollow shell particle, allowing the cell to accumulate biomass, selecting hollow shell particles based on accumulated biomass, and degrading the hollow shell particles to release the cells and develop hyper-producing cell lines. Exemplary cell types include microalgae, mammalian cells, bacteria, yeast, and fungi.