公开(公告)号：US20160266087A1

公开(公告)日：2016-09-15

申请号：US15064985

申请日：2016-03-09

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Joe Fu-Jiou LO ,  Zhengtuo ZHAO ,  Rui LIU

IPC: G01N33/483 ,  G01N21/64

CPC classification number: G01N33/4833 ,  A61B3/00 ,  A61B3/10 ,  A61B5/00 ,  A61B5/0066 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/443 ,  G01N21/6408 ,  G01N21/6486 ,  G01N2021/6463 ,  G01N2021/6484 ,  G01N2201/062 ,  G01N2201/088 ,  G01N2333/78

Abstract: Devices and methods for performing frequency domain (FD) fluorescence lifetime spectroscopy are provided. The devices include a modulated light source, a focusing optical fiber, a detecting optical fiber, and a detector. The methods include focusing sinusoidal modulated incident light from a light source on a biological sample containing a protein, detecting a range of wavelengths of sinusoidal modulated fluorescent light emitted from the protein, determining a phase shift for the modulated fluorescent light, determining an amplitude modulation of the modulated fluorescent light, and determining a fluorescence lifetime of the protein.

Abstract translation: 提供了用于执行频域（FD）荧光寿命光谱的装置和方法。 该装置包括调制光源，聚焦光纤，检测光纤和检测器。 所述方法包括将来自光源的正弦调制入射光聚焦在含有蛋白质的生物样品上，检测从蛋白质发射的正弦调制荧光的波长范围，确定调制荧光的相移，确定调制荧光的幅度调制 调制荧光，并测定蛋白质的荧光寿命。