公开(公告)号：US11084849B2

公开(公告)日：2021-08-10

申请号：US16073602

申请日：2017-01-27

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Tom W. Muir ,  Adam J. Stevens ,  Neel H. Shah

IPC: C07K14/00 ,  A61K47/68 ,  C07K14/32 ,  C07K16/28 ,  C12N15/62

Abstract: Embodiments of the present invention relate to inteins, split inteins, compositions comprising inteins and methods for use of these.

公开(公告)号：US20200087388A1

公开(公告)日：2020-03-19

申请号：US16690213

申请日：2019-11-21

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Tom W. Muir ,  Miquel Vila-Perello ,  Zhihua Liu ,  Neel H. Shah

IPC: C07K16/18 ,  C07K14/195 ,  C12P21/02 ,  C12P21/06 ,  C12N9/00 ,  C12N15/62

Abstract: Disclosed herein are split inteins, fused proteins of split inteins, and methods of using split inteins to efficiently purify and modify proteins of interest.

公开(公告)号：US12168671B2

公开(公告)日：2024-12-17

申请号：US17707330

申请日：2022-03-29

Applicant: The Trustees of Princeton University

Inventor： Tom W. Muir ,  Rob C. Oslund ,  Jung-Min Kee

IPC: C07F9/6503 ,  C07K16/44 ,  G01N33/53

Abstract: Provided are phosphonopyrazole-based phosphohistidine analogs that are useful as haptens for the preparation of immunogens, immunogens that include these haptens linked to carrier molecules, antibodies thereto and uses of these antibodies, haptens, immunogens and phosphohistidine analogs.

公开(公告)号：US20190092796A1

公开(公告)日：2019-03-28

申请号：US16135396

申请日：2018-09-19

Applicant: The Trustees of Princeton University

Inventor： Tom W. Muir ,  Rob C. Oslund ,  Jung-Min Kee

IPC: C07F9/6503 ,  G01N33/53 ,  C07K16/44

Abstract: Provided are phosphonopyrazole-based phosphohistidine analogs that are useful as haptens for the preparation of immunogens, immunogens that include these haptens linked to carrier molecules, antibodies thereto and uses of these antibodies, haptens, immunogens and phosphohistidine analogs.

公开(公告)号：US10526401B2

公开(公告)日：2020-01-07

申请号：US15809440

申请日：2017-11-10

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Tom W. Muir ,  Miquel Vila-Perello ,  Zhihua Liu ,  Neel H. Shah

IPC: C12P21/02 ,  C12P21/06 ,  C12N15/62 ,  C07K16/18 ,  C07K14/195 ,  C12N9/00

Abstract: Disclosed herein are split inteins, fused proteins of split inteins, and methods of using split inteins to efficiently purify and modify proteins of interest.

公开(公告)号：US10087485B2

公开(公告)日：2018-10-02

申请号：US14405303

申请日：2013-06-06

Applicant: The Trustees of Princeton University

Inventor： Tom W. Muir ,  Uyen T. T. Nguyen ,  Manuel M. Mueller

IPC: C40B20/04 ,  C12Q1/6876 ,  C12Q1/48 ,  G01N33/68 ,  C40B30/08 ,  C40B30/04 ,  C12N15/10

Abstract: Compositions and methods are provided for DNA barcoding of designer mononucleosome and polynucleosome (chromatin array) libraries for use, for example, for the profiling of chromatin readers, writers, erasers, and modulators thereof.

公开(公告)号：US20180057577A1

公开(公告)日：2018-03-01

申请号：US15809440

申请日：2017-11-10

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Tom W. Muir ,  Miquel Vila-Perello ,  Zhihua Liu ,  Neel H. Shah

IPC: C07K16/18 ,  C12N15/62 ,  C12N9/00 ,  C12P21/06 ,  C12P21/02 ,  C07K14/195

Abstract: Disclosed herein are split inteins, fused proteins of split inteins, and methods of using split inteins to efficiently purify and modify proteins of interest.

公开(公告)号：US20160311838A1

公开(公告)日：2016-10-27

申请号：US15026670

申请日：2014-10-02

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Tom W. Muir ,  Rob C. Oslund ,  Jung-Min Kee

IPC: C07F9/6503 ,  G01N33/53 ,  C07K16/44

CPC classification number: C07F9/65031 ,  C07K16/44 ,  G01N33/5308 ,  G01N2800/26

Abstract: Provided are phosphonopyrazole-based phosphohistidine analogs that are useful as haptens for the preparation of immunogens, immunogens that include these haptens linked to carrier molecules, antibodies thereto and uses of these antibodies, haptens, immunogens and phosphohistidine analogs. The invention is based in part on new pHis analogs that are chemically stable and which have been used as haptens and immunogens to generate specific anti-pHis antibodies that bind with high affinity and specificity.

Abstract translation: 提供了可用作制备免疫原的半抗原的基于膦酰吡唑的磷酸组氨酸类似物，包括与载体分子连接的这些半抗原的免疫原，其抗体及其抗体，半抗原，免疫原和磷酸组氨酸类似物的用途。 本发明部分基于化学稳定的新的pH值类似物，并且其已被用作半抗原和免疫原以产生以高亲和性和特异性结合的特异性抗pH值抗体。

公开(公告)号：US12297472B2

公开(公告)日：2025-05-13

申请号：US17363698

申请日：2021-06-30

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Tom W. Muir ,  Adam J. Stevens ,  Neel H. Shah

IPC: C12N9/00 ,  C07K14/00 ,  C07K14/32 ,  C07K16/28 ,  C12N15/62

Abstract: Embodiments of the present invention relate to inteins, split inteins, compositions comprising inteins and methods for use of these.

公开(公告)号：US12054541B2

公开(公告)日：2024-08-06

申请号：US17491729

申请日：2021-10-01

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Tom W. Muir ,  Miquel Vila-Perello ,  Zhihua Liu ,  Neel H. Shah

IPC: C07K14/195 ,  C07K16/18 ,  C12N9/00 ,  C12N15/62 ,  C12P21/02 ,  C12P21/06

CPC classification number: C07K16/18 ,  C07K14/195 ,  C12N9/93 ,  C12N15/62 ,  C12P21/02 ,  C12P21/06 ,  C07K2319/50 ,  C07K2319/92

Abstract: Disclosed herein are split inteins, fused proteins of split inteins, and methods of using split inteins to efficiently purify and modify proteins of interest.