公开(公告)号：US20200095331A1

公开(公告)日：2020-03-26

申请号：US16695674

申请日：2019-11-26

申请人： The United States of America,as represented by the Secretary,Department of Health and Human Services

发明人： Hisataka Kobayashi ,  Peter Choyke

IPC分类号： C07K16/30 ,  A61K49/00 ,  A61K41/00 ,  A61K47/68 ,  C07K16/28 ,  C07K16/32

摘要： The present disclosure relates to compositions and methods of killing cells in vitro or in vivo. In particular examples, the method includes contacting a cell having a cell surface protein with a therapeutically effective amount of an antibody-IR700 molecule, wherein the antibody specifically binds to the cell surface protein. In particular examples the antibody recognizes a tumor-specific antigen on the surface of a tumor cell. The cell is subsequently irradiated, such as at a wavelength of 660 to 740 nm at a dose of at least 1 J cm−2, thereby killing the cell. Also provided are wearable devices that include an article of clothing, jewelry, or covering; and an NIR LED incorporated into the article, which can be used with the disclosed methods.

公开(公告)号：US11781955B2

公开(公告)日：2023-10-10

申请号：US17026724

申请日：2020-09-21

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Hisataka Kobayashi ,  Peter Choyke ,  Martin John Schnermann

IPC分类号： G01N1/44 ,  A61K47/68 ,  A61K49/00 ,  A61K41/00 ,  A61N5/06 ,  G01N1/40 ,  G01N33/50

CPC分类号： G01N1/44 ,  A61K41/0071 ,  A61K47/6891 ,  A61K49/0036 ,  A61N5/062 ,  G01N1/405 ,  G01N33/5002 ,  A61N2005/0651 ,  A61N2005/0663

摘要： This disclosure provides IR700-molecule conjugates and methods of their use to remove (e.g., separate or isolate) a target from a sample in vivo or from a subject in vitro. It is shown herein that exposure of IR700 to near infrared (NIR) light removes a portion of IR700, changing it from a hydrophilic molecule, to one that is hydrophobic, resulting in aggregation of IR700 and anything bound to it. For example, the disclosed IR700-molecule conjugates and methods provide photo-controlled ways to control the pharmacokinetics of a drug in vivo, and can be used to remove undesired agents from environmental or food samples or to isolate target molecules in a laboratory.

公开(公告)号：US10830678B2

公开(公告)日：2020-11-10

申请号：US15318104

申请日：2015-08-07

申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： Hisataka Kobayashi ,  Peter Choyke ,  Martin John Schnermann

IPC分类号： G01N1/44 ,  A61N5/06 ,  G01N1/40 ,  G01N33/50 ,  A61K49/00 ,  A61K41/00 ,  A61K47/68

摘要： This disclosure provides IR700-molecule conjugates and methods of their use to remove (e.g., separate or isolate) a target from a sample in vivo or from a subject in vitro. It is shown herein that exposure of IR700 to near infrared (NIR) light removes a portion of IR700, changing it from a hydrophilic molecule, to one that is hydrophobic, resulting in aggregation of IR700 and anything bound to it. For example, the disclosed IR700-molecule conjugates and methods provide photo-controlled ways to control the pharmacokinetics of a drug in vivo, and can be used to remove undesired agents from environmental or food samples or to isolate target molecules in a laboratory.

公开(公告)号：US10537641B2

公开(公告)日：2020-01-21

申请号：US14868040

申请日：2015-09-28

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Hisataka Kobayashi ,  Peter Choyke

IPC分类号： A61K39/00 ,  A61K49/16 ,  A61K41/00 ,  A61K31/704 ,  A61K39/395 ,  A61K45/06 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  G01N33/50 ,  A61K47/68 ,  A44C15/00 ,  A41D13/00

摘要： The present disclosure relates to compositions and methods of killing cells. In particular examples, the method includes contacting a cell having a cell surface protein with a therapeutically effective amount of an antibody-IR700 molecule, wherein the antibody specifically binds to the cell surface protein, such as a tumor-specific antigen on the surface of a tumor cell. The cell is subsequently irradiated, such as at a wavelength of 660 to 740 nm at a dose of at least 1 J cm−2. The cell is also contacted with one or more therapeutic agents (such as an anti-cancer agent), for example about 0 to 8 hours after irradiating the cell, thereby killing the cell. Also provided are methods of imaging cell killing in real time, using fluorescence lifetime imaging. Also provided are wearable devices that include an article of clothing, jewelry, or covering; and an NIR LED incorporated into the article, which can be used with the disclosed methods.

公开(公告)号：US11200667B2

公开(公告)日：2021-12-14

申请号：US16488127

申请日：2018-02-22

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Nathan S. Lay ,  Yohannes Tsehay ,  Ronald M. Summers ,  Baris Turkbey ,  Matthew Greer ,  Ruida Cheng ,  Holger Roth ,  Matthew J. McAuliffe ,  Sonia Gaur ,  Francesca Mertan ,  Peter Choyke

IPC分类号： G06T7/00 ,  G06N20/00

摘要： Disclosed prostate computer aided diagnosis (CAD) systems employ a Random Forest classifier to detect prostate cancer. System classify individual pixels inside the prostate as potential sites of cancer using a combination of spatial, intensity and texture features extracted from three sequences. The Random Forest training considers instance-level weighting for equal treatment of small and large cancerous lesions and small and large prostate backgrounds. Two other approaches are based on an AutoContext pipeline intended to make better use of sequence-specific patterns. Also disclosed are methods and systems for accurate automatic segmentation of the prostate in MRI. Methods can include both patch-based and holistic (image-to-image) deep learning methods for segmentation of the prostate. A patch-based convolutional network aims to refine the prostate contour given an initialization. A method for end- to-end prostate segmentation integrates holistically nested edge detection with fully convolutional networks. HNNs automatically learn a hierarchical representation that improve prostate boundary detection.

公开(公告)号：US10538590B2

公开(公告)日：2020-01-21

申请号：US13972532

申请日：2013-08-21

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Hisataka Kobayashi ,  Peter Choyke

IPC分类号： A61K39/00 ,  A61K49/16 ,  A61K41/00 ,  A61K31/704 ,  A61K39/395 ,  A61K45/06 ,  C07K16/28 ,  C07K16/30 ,  A61K49/00 ,  C07K16/32 ,  A61K47/68 ,  A44C15/00 ,  A41D13/00

摘要： The present disclosure relates to compositions and methods of killing cells in vitro or in vivo. In particular examples, the method includes contacting a cell having a cell surface protein with a therapeutically effective amount of an antibody-IR700 molecule, wherein the antibody specifically binds to the cell surface protein. In particular examples the antibody recognizes a tumor-specific antigen on the surface of a tumor cell. The cell is subsequently irradiated, such as at a wavelength of 660 to 740 nm at a dose of at least 1 J cm−2, thereby killing the cell. Also provided are wearable devices that include an article of clothing, jewelry, or covering; and an NIR LED incorporated into the article, which can be used with the disclosed methods.

公开(公告)号：US11364298B2

公开(公告)日：2022-06-21

申请号：US16695674

申请日：2019-11-26

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Hisataka Kobayashi ,  Peter Choyke

IPC分类号： A61K31/704 ,  A61K39/395 ,  A61K41/00 ,  A61K49/16 ,  A61K47/68 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  A61K45/06 ,  G01N33/50 ,  A61K49/00 ,  A44C15/00 ,  A41D13/00 ,  A61K39/00

摘要： The present disclosure relates to compositions and methods of killing cells in vitro or in vivo. In particular examples, the method includes contacting a cell having a cell surface protein with a therapeutically effective amount of an antibody-IR700 molecule, wherein the antibody specifically binds to the cell surface protein. In particular examples the antibody recognizes a tumor-specific antigen on the surface of a tumor cell. The cell is subsequently irradiated, such as at a wavelength of 660 to 740 nm at a dose of at least 1 J cm−2, thereby killing the cell. Also provided are wearable devices that include an article of clothing, jewelry, or covering; and an NIR LED incorporated into the article, which can be used with the disclosed methods.

公开(公告)号：US11364297B2

公开(公告)日：2022-06-21

申请号：US16694761

申请日：2019-11-25

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Hisataka Kobayashi ,  Peter Choyke

IPC分类号： A61K31/704 ,  A61K39/395 ,  A61K41/00 ,  A61K49/16 ,  A61K47/68 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  A61K45/06 ,  G01N33/50 ,  A61K49/00 ,  A44C15/00 ,  A41D13/00 ,  A61K39/00

摘要： The present disclosure relates to compositions and methods of killing cells. In particular examples, the method includes contacting a cell having a cell surface protein with a therapeutically effective amount of an antibody-IR700 molecule, wherein the antibody specifically binds to the cell surface protein, such as a tumor-specific antigen on the surface of a tumor cell. The cell is subsequently irradiated, such as at a wavelength of 660 to 740 nm at a dose of at least 1 J cm−2. The cell is also contacted with one or more therapeutic agents (such as an anti-cancer agent), for example about 0 to 8 hours after irradiating the cell, thereby killing the cell. Also provided are methods of imaging cell killing in real time, using fluorescence lifetime imaging. Also provided are wearable devices that include an article of clothing, jewelry, or covering; and an NIR LED incorporated into the article, which can be used with the disclosed methods.

公开(公告)号：US20160256564A2

公开(公告)日：2016-09-08

申请号：US14868040

申请日：2015-09-28

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Hisataka Kobayashi ,  Peter Choyke

IPC分类号： A61K47/48 ,  A61K41/00 ,  A61K39/395 ,  C07K16/40 ,  A61K45/06

CPC分类号： A61K45/06 ,  A41D13/0002 ,  A41D2400/32 ,  A44C15/00 ,  A61K31/704 ,  A61K41/0071 ,  A61K47/6803 ,  A61K47/6869 ,  A61K2039/505 ,  C07K16/2863 ,  C07K16/3069 ,  C07K16/32 ,  C07K2317/24 ,  C07K2317/73 ,  C07K2317/77 ,  G01N33/5011 ,  A61K2300/00

摘要： The present disclosure relates to compositions and methods of killing cells. In particular examples, the method includes contacting a cell having a cell surface protein with a therapeutically effective amount of an antibody-IR700 molecule, wherein the antibody specifically binds to the cell surface protein, such as a tumor-specific antigen on the surface of a tumor cell. The cell is subsequently irradiated, such as at a wavelength of 660 to 740 nm at a dose of at least 1 J cm−2. The cell is also contacted with one or more therapeutic agents (such as an anti-cancer agent), for example about 0 to 8 hours after irradiating the cell, thereby killing the cell. Also provided are methods of imaging cell killing in real time, using fluorescence lifetime imaging. Also provided are wearable devices that include an article of clothing, jewelry, or covering; and an NIR LED incorporated into the article, which can be used with the disclosed methods.

摘要翻译： 本公开涉及杀死细胞的组合物和方法。 在具体实例中，该方法包括使具有细胞表面蛋白的细胞与治疗有效量的抗体-IR700分子接触，其中该抗体特异性结合细胞表面蛋白，例如表面上的肿瘤特异性抗原 肿瘤细胞。 随后以至少1J cm -2的剂量照射细胞，例如在660至740nm的波长处。 细胞还与一种或多种治疗剂（例如抗癌剂）接触，例如在照射细胞后约0至8小时，从而杀死细胞。 还提供了使用荧光寿命成像实时成像细胞杀伤的方法。 还提供了可穿戴装置，其包括衣物，饰品或覆盖物; 以及纳入该制品中的NIR LED，其可以与所公开的方法一起使用。