公开(公告)号：US20230323310A1

公开(公告)日：2023-10-12

申请号：US18043507

申请日：2021-09-03

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Tomoko KUNITOMI ,  Shiro KITANO ,  Kei TSUKAMOTO ,  Michiya MATSUSAKI

IPC: C12N5/071 ,  C12N5/077

CPC classification number: C12N5/069 ,  C12N5/0656 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2501/91

Abstract: The present invention relates to a method for producing a three-dimensional tissue body, including: a step of obtaining a mixture by mixing a cationic substance and a fragmented extracellular matrix component with cells; and a step of culturing the cells after the step of obtaining the mixture.

公开(公告)号：US20230203452A1

公开(公告)日：2023-06-29

申请号：US18171832

申请日：2023-02-21

Applicant: TOPPAN Inc.

Inventor： Kei TSUKAMOTO ,  Shiro KITANO ,  Tomoko KUNITOMI

IPC: C12N5/09

CPC classification number: C12N5/0693 ,  C12N2502/28 ,  C12N2502/1323 ,  C12N2513/00 ,  C12N2533/90

Abstract: A method of producing a conditioned medium for culturing a patient-derived cancer cell, including culturing, in a first medium for 24 hours or longer, a three-dimensional cell tissue including an extracellular matrix component, a polymer electrolyte, and a cell cluster including a fibroblast, and collecting the first medium in which the three-dimensional cell tissue has been cultured as the conditioned medium.

公开(公告)号：US20230117082A1

公开(公告)日：2023-04-20

申请号：US18067264

申请日：2022-12-16

Applicant: TOPPAN Inc.

Inventor： Yoichi MAKINO ,  Akihiro HOSHINO ,  Tomoko KUNITOMI ,  Keisuke GOTO ,  Yosuke HORIUCHI

IPC: B01L3/00 ,  G01N21/64

Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that the interface is formed at a distance not less than a fluorescence-obtainable distance from a bottom of the receiving sections.