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公开(公告)号：US09018450B2
公开(公告)日：2015-04-28
申请号：US14279946
申请日：2014-05-16
Applicant: Toyota Jidosha Kabushiki Kaisha
Inventor： Hiroshi Chatani , Chikara Ohto , Yukio Okamura , Norihiro Mitsukawa , Nobuhiko Muramoto , Masaru Takagi , Nobutaka Mitsuda , Tomotsugu Koyama , Kyoko Matsui
CPC classification number: C12N15/8247 , C07K7/06 , C07K14/415 , C12N15/8216 , C12N15/8217 , C12N15/8245 , C12N15/8261 , Y02A40/146
Abstract: This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.
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公开(公告)号：US10584348B2
公开(公告)日：2020-03-10
申请号：US14700763
申请日：2015-04-30
Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
Inventor： Satoshi Kondo , Chikara Ohto , Hiroki Sugimoto , Nobuhiko Muramoto , Tomoko Tanaka , Norihiro Mitsukawa , Ritsuko Yogo
IPC: C12N15/82
Abstract: This invention is intended to increase biomass production through suppression of expression of a gene functioning in relation to intracellular vesicular transport or inhibition of functions of a protein encoded by such gene. To this end, expression of a gene encoding the coatomer adapter zeta subunit is suppressed, the coatomer adapter zeta subunit is inhibited, expression of a gene encoding the clathrin adaptor small (sigma) subunit is suppressed, or the clathrin adaptor small (sigma) subunit is inhibited.
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公开(公告)号：US09856488B2
公开(公告)日：2018-01-02
申请号：US14976233
申请日：2015-12-21
Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
Inventor： Madoka Yonekura , Chikara Ohto , Nobuhiko Muramoto , Norihiro Mitsukawa , Masaru Takagi , Kyoko Matsui
IPC: C12N15/82 , C07K14/415
CPC classification number: C12N15/8251 , C07K14/415
Abstract: According to the present invention, a gene having a novel function that can cause an increase or decrease in seed protein content is searched for. A chimeric protein obtained by fusing a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 1 to 76 and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor or a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 77 to 84 is expressed in a plant.
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公开(公告)号：US09309530B2
公开(公告)日：2016-04-12
申请号：US14815295
申请日：2015-07-31
Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
Inventor： Satoshi Kondo , Chikara Ohto , Nobuhiko Muramoto , Norihiro Mitsukawa , Masaru Takagi , Kyoko Matsui
IPC: C12N15/82 , C12N5/14 , C07K14/415 , A01H5/02
CPC classification number: C12N15/8247 , A01H5/02 , C07K14/415 , C12N15/8216 , C12N15/8217
Abstract: An object of the present invention is to search for a gene having a novel function that can cause an increase or decrease in material productivity, and particularly, fat and oil content. In the present invention, a chimeric protein obtained by fusing a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 1 to 158 and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor is expressed in a plant.
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公开(公告)号：US09068193B2
公开(公告)日：2015-06-30
申请号：US14280063
申请日：2014-05-16
Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
Inventor： Hiroshi Chatani , Chikara Ohto , Yukio Okamura , Norihiro Mitsukawa , Nobuhiko Muramoto , Masaru Takagi , Nobutaka Mitsuda , Tomotsugu Koyama , Kyoko Matsui
CPC classification number: C12N15/8247 , C07K7/06 , C07K14/415 , C12N15/8216 , C12N15/8217 , C12N15/8245 , C12N15/8261 , Y02A40/146
Abstract: This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.
Abstract translation: 本发明旨在用于搜索具有增加单个植物的重量，增加每个植物的给定组织的重量或提高每个植物的给定物质的生产力的新功能的转录因子并改善 植物中的这种特性。 单个植物的重量增加，每个植物的给定组织的重量增加，每个植物的给定物质的生产力得到改善，或者通过表达增加每个给定组织的给定物质的含量 已被修饰以抑制转录加速活性的转录因子。
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Patent Agency Ranking
公开(公告)号：US09012727B2
公开(公告)日：2015-04-21
申请号：US14280163
申请日：2014-05-16
Applicant: Toyota Jidosha Kabushiki Kaisha
Inventor： Hiroshi Chatani , Chikara Ohto , Yukio Okamura , Norihiro Mitsukawa , Nobuhiko Muramoto , Masaru Takagi , Nobutaka Mitsuda , Tomotsugu Koyama , Kyoko Matsui
CPC classification number: C12N15/8247 , C07K7/06 , C07K14/415 , C12N15/8216 , C12N15/8217 , C12N15/8245 , C12N15/8261 , Y02A40/146
Abstract: This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.
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公开(公告)号：US08969657B2
公开(公告)日：2015-03-03
申请号：US14280118
申请日：2014-05-16
Applicant: Toyota Jidosha Kabushiki Kaisha
Inventor： Hiroshi Chatani , Chikara Ohto , Yukio Okamura , Norihiro Mitsukawa , Nobuhiko Muramoto , Masaru Takagi , Nobutaka Mitsuda , Tomotsugu Koyama , Kyoko Matsui
Abstract: This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.
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公开(公告)号：US20150059020A1
公开(公告)日：2015-02-26
申请号：US14389127
申请日：2013-04-17
Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA , OKAYAMA PREFECTURE
Inventor： Satoshi Kondo , Chikara Ohto , Kenichi Ogawa , Kenji Henmi
CPC classification number: C12N15/8273 , A01H1/04 , C12N9/16 , C12N15/8271 , C12Y301/03048
Abstract: Environmental stress resistance is imparted to a plant or the environmental stress resistance of a plant is improved. Environmental stress resistance is imparted to a plant by changing the expression level of the tyrosine phosphatase gene (At1g71860).
Abstract translation: 赋予植物耐环境胁迫或提高植物的耐环境胁迫性。 通过改变酪氨酸磷酸酶基因的表达水平（At1g71860），赋予植物环境胁迫抗性。
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公开(公告)号：US11464182B2
公开(公告)日：2022-10-11
申请号：US15740312
申请日：2016-07-01
Inventor： Nobuhiko Muramoto , Hidenori Tanaka , Hiroki Sugimoto , Norihiro Mitsukawa , Akinori Ikeuchi , Risa Nakamura , Ritsuko Yogo , Satoshi Katahira , Chikara Ohto , Satoshi Kondo
Abstract: A method of inducing genetic recombination, including: allowing a protein having DNA double-stranded cleavage activity to act in cells of a eukaryotic organism which is a polyploidy inherently possessed by a eukaryotic organism. In eukaryotic organisms, various genetic recombination generates new genome set composition. This is done to obtain a population of eukaryotic organisms that hold the modified genomic set.
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公开(公告)号：US10377993B2
公开(公告)日：2019-08-13
申请号：US14716973
申请日：2015-05-20
Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA , KYOTO UNIVERSITY
Inventor： Chikara Ohto , Masayoshi Muramatsu , Masakazu Ito , Jun Ogawa , Shigenobu Kishino
Abstract: This invention provides a recombinant microorganism into which an acyl-CoA reductase exhibiting excellent activity in a reduction reaction using acyl-CoA as a substrate has been introduced. Such recombinant microorganism comprises a nucleic acid encoding an acyl-CoA reductase comprising any of characteristic Common sequences 1 to 3 introduced into a host microorganism.
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