公开(公告)号：US20170159044A1

公开(公告)日：2017-06-08

申请号：US15433909

申请日：2017-02-15

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Esteban Toro ,  Sebastian TREUSCH ,  Siyuan CHEN ,  Cheng-Hsien WU

IPC: C12N15/10 ,  C12P19/34

CPC classification number: C12N15/1031 ,  C12N15/1027 ,  C12N15/1093 ,  C12N15/66 ,  C12P19/34

Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.

公开(公告)号：US20220315971A1

公开(公告)日：2022-10-06

申请号：US17825863

申请日：2022-05-26

Applicant: Twist Bioscience Corporation

Inventor： Cheng-Hsien WU ,  Sebastian TREUSCH

IPC: C12P19/34

Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Such methods, systems, and compositions for seamless nucleic acid assembly include those for in vitro recombination cloning, single-stranded hierarchal DNA assembly, or overlap extension PCR without primer removal.

公开(公告)号：US20200181667A1

公开(公告)日：2020-06-11

申请号：US16712678

申请日：2019-12-12

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Cheng-Hsien WU ,  Sebastian TREUSCH

IPC: C12P19/34

Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Such methods, systems, and compositions for seamless nucleic acid assembly include those for in vitro recombination cloning, single-stranded hierarchal DNA assembly, or overlap extension PCR without primer removal.

公开(公告)号：US20240158955A1

公开(公告)日：2024-05-16

申请号：US18356787

申请日：2023-07-21

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Anthony COX ,  Sebastian TREUSCH ,  Siyuan CHEN

IPC: C40B50/06 ,  C12N15/10 ,  C12N15/113 ,  C12Q1/6876 ,  C40B50/08 ,  C40B50/14 ,  G01N33/50

CPC classification number: C40B50/06 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/113 ,  C12Q1/6876 ,  C40B50/08 ,  C40B50/14 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5041 ,  C12N2320/30 ,  C12N2330/31 ,  C12Q1/6886

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

公开(公告)号：US20220064628A1

公开(公告)日：2022-03-03

申请号：US17320127

申请日：2021-05-13

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Esteban Toro ,  Sebastian TREUSCH ,  Siyuan CHEN ,  Cheng-Hsien WU

IPC: C12N15/10 ,  C12P19/34 ,  C12N15/66

Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.

公开(公告)号：US20190352635A1

公开(公告)日：2019-11-21

申请号：US16530717

申请日：2019-08-02

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Esteban Toro ,  Sebastian TREUSCH ,  Siyuan CHEN ,  Cheng-Hsien WU

IPC: C12N15/10 ,  C12N15/66 ,  C12P19/34

Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.

公开(公告)号：US20170081660A1

公开(公告)日：2017-03-23

申请号：US15268422

申请日：2016-09-16

Applicant: Twist Bioscience Corporation

Inventor： Anthony COX ,  Sebastian TREUSCH ,  Siyuan CHEN

IPC: C12N15/10 ,  C12N15/113 ,  C12Q1/68 ,  G01N33/50

CPC classification number: C12N15/1093 ,  C12N15/1079 ,  C12N15/113 ,  C12N2320/30 ,  C12N2330/31 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q2600/158 ,  C40B50/08 ,  C40B50/14 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5041

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.