公开(公告)号：US20210180046A1

公开(公告)日：2021-06-17

申请号：US17183038

申请日：2021-02-23

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Anthony COX ,  Siyuan CHEN

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6816

Abstract: Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

公开(公告)号：US20180051278A1

公开(公告)日：2018-02-22

申请号：US15682100

申请日：2017-08-21

Applicant: Twist Bioscience Corporation

Inventor： Anthony COX ,  Siyuan CHEN

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1068 ,  C12N15/1093 ,  C12N2310/10 ,  C12N2310/20 ,  C12Q1/6806 ,  C12Q1/6816 ,  C40B40/06

Abstract: Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

公开(公告)号：US20170081660A1

公开(公告)日：2017-03-23

申请号：US15268422

申请日：2016-09-16

Applicant: Twist Bioscience Corporation

Inventor： Anthony COX ,  Sebastian TREUSCH ,  Siyuan CHEN

IPC: C12N15/10 ,  C12N15/113 ,  C12Q1/68 ,  G01N33/50

CPC classification number: C12N15/1093 ,  C12N15/1079 ,  C12N15/113 ,  C12N2320/30 ,  C12N2330/31 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q2600/158 ,  C40B50/08 ,  C40B50/14 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5041

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

公开(公告)号：US20240158955A1

公开(公告)日：2024-05-16

申请号：US18356787

申请日：2023-07-21

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Anthony COX ,  Sebastian TREUSCH ,  Siyuan CHEN

IPC: C40B50/06 ,  C12N15/10 ,  C12N15/113 ,  C12Q1/6876 ,  C40B50/08 ,  C40B50/14 ,  G01N33/50

CPC classification number: C40B50/06 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/113 ,  C12Q1/6876 ,  C40B50/08 ,  C40B50/14 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5041 ,  C12N2320/30 ,  C12N2330/31 ,  C12Q1/6886

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

公开(公告)号：US20210040476A1

公开(公告)日：2021-02-11

申请号：US17068551

申请日：2020-10-12

Applicant: Twist Bioscience Corporation

Inventor： Anthony COX ,  Sebastian Treusch ,  Siyuan CHEN

IPC: C12N15/10 ,  G01N33/50 ,  C40B50/14 ,  C40B50/08 ,  C12N15/113 ,  C12Q1/6876

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

公开(公告)号：US20200299684A1

公开(公告)日：2020-09-24

申请号：US16759282

申请日：2018-10-26

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Esteban TORO ,  James DIGGANS ,  Siyuan CHEN ,  Anthony COX ,  Arthur VIGIL ,  Kieran HERVOLD

IPC: C12N15/10 ,  G16B30/20 ,  G16B30/10

Abstract: The present disclosure describes software tools for predicting the feasibility of synthesizing and assembling polynucleotides. Polynucleotide scoring tools describe herein provide automated methods for predicting efficient strategies and reaction conditions for synthesizing and assembling polynucleotides.

公开(公告)号：US20180171509A1

公开(公告)日：2018-06-21

申请号：US15844395

申请日：2017-12-15

Applicant: Twist Bioscience Corporation

Inventor： Anthony COX ,  Siyuan Chen

IPC: C40B40/08 ,  G01N33/68 ,  G01N33/50 ,  C12N15/10 ,  A61K39/00

Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a TCR or a TCR-binding antigen. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

公开(公告)号：US20210115594A1

公开(公告)日：2021-04-22

申请号：US17133408

申请日：2020-12-23

Applicant: Twist Bioscience Corporation

Inventor： Anthony COX ,  Siyuan CHEN

IPC: C40B40/08 ,  C07K14/725 ,  G01N33/574 ,  G01N33/68 ,  A61K39/00 ,  C12N15/10 ,  G01N33/50

Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a TCR or a TCR-binding antigen. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

公开(公告)号：US20210102198A1

公开(公告)日：2021-04-08

申请号：US17120037

申请日：2020-12-11

Applicant: Twist Bioscience Corporation

Inventor： Anthony COX ,  Siyuan CHEN

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a reference domain of a CAR. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

公开(公告)号：US20180282721A1

公开(公告)日：2018-10-04

申请号：US15921479

申请日：2018-03-14

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Anthony COX ,  Siyuan CHEN ,  Charles LEDOGAR ,  Dominique TOPPANI

IPC: C12N15/10

Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.