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公开(公告)号:US07074616B1
公开(公告)日:2006-07-11
申请号:US09367052
申请日:1997-02-07
申请人: Tadamitsu Kishimoto , Takashi Nagasawa , Kazunobu Tachibana , Hisashi Iizasa , Nobuaki Yoshida , Toshihiro Nakajima , Osamu Yoshie
发明人: Tadamitsu Kishimoto , Takashi Nagasawa , Kazunobu Tachibana , Hisashi Iizasa , Nobuaki Yoshida , Toshihiro Nakajima , Osamu Yoshie
CPC分类号: C07K14/7158 , A61K38/00
摘要: The present invention pertains to a DNA encoding a polypeptide comprising an entire sequence of the amino acid sequence as shown by SEQ ID NO:2 of Sequence Listing or a partial sequence thereof, or a polypeptide comprising the polypeptide described above, wherein any of the polypeptides has an activity of a receptor capable of binding to a murine PBSF/SDF-1; a polypeptide encoded by the DNA described above, wherein the polypeptide has an activity of a receptor capable of binding to a murine PBSF/SDF-1; cells expressing the polypeptide described above and a human CD4 protein; and a method of screening an AIDS onset inhibitor or an HIV-1 infection inhibitor, characterized by the use of the cells described above. According to the present invention, there can be provided a novel murine CXC chemokine receptor gene, a method of screening an HIV-1 infection inhibitor, and the like, each of which is useful in studies of a therapeutic agent for AIDS and the functional mechanism of HIV-1 infection.
摘要翻译: 本发明涉及编码多肽的DNA,其包含序列表的SEQ ID NO:2所示的氨基酸序列的整个序列或其部分序列,或包含上述多肽的多肽,其中任何多肽 具有能够结合小鼠PBSF / SDF-1的受体的活性; 由上述DNA编码的多肽,其中所述多肽具有能够结合小鼠PBSF / SDF-1的受体的活性; 表达上述多肽的细胞和人CD4蛋白; 以及筛选艾滋病起病抑制剂或HIV-1感染抑制剂的方法,其特征在于使用上述细胞。 根据本发明,可以提供新的鼠CXC趋化因子受体基因,筛选HIV-1感染抑制剂的方法等,其可用于AIDS治疗剂的研究和功能机制 的HIV-1感染。
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公开(公告)号:US07361737B2
公开(公告)日:2008-04-22
申请号:US11437734
申请日:2006-05-22
申请人: Tadamitsu Kishimoto , Takashi Nagasawa , Kazunobu Tachibana , Hisashi Iizasa , Nobuaki Yoshida , Toshihiro Nakajima , Osamu Yoshie
发明人: Tadamitsu Kishimoto , Takashi Nagasawa , Kazunobu Tachibana , Hisashi Iizasa , Nobuaki Yoshida , Toshihiro Nakajima , Osamu Yoshie
IPC分类号: C07K14/00 , C07K14/47 , C07K14/705 , C07H21/00 , C07H21/04
CPC分类号: C07K14/7158 , A61K38/00
摘要: The present invention pertains to a DNA encoding a polypeptide including an entire sequence of the amino acid sequence as shown by SEQ ID NO: 2 or a partial sequence thereof, or a polypeptide including the polypeptide described above, wherein any of the polypeptides has an activity of a receptor capable of binding to a murine PBSF/SDF-1; a polypeptide encoded by the DNA described above, wherein the polypeptide has an activity of a receptor capable of binding to a murine PBSF/SDF-1; cells expressing the polypeptide described above and a human CD4 protein; and a method of screening an AIDS onset inhibitor or an HIV-1 infection inhibitor, characterized by the use of the cells described above. According to the present invention, there can be provided a novel murine CXC chemokine receptor gene, a method of screening an HIV-1 infection inhibitor, and the like, each of which is useful in studies of a therapeutic agent for AIDS and the functional mechanism of HIV-1 infection.
摘要翻译: 本发明涉及编码包含如SEQ ID NO:2或其部分序列所示的氨基酸序列的整个序列或包含上述多肽的多肽的多肽的DNA,其中任何多肽具有活性 的能够结合小鼠PBSF / SDF-1的受体; 由上述DNA编码的多肽,其中所述多肽具有能够结合小鼠PBSF / SDF-1的受体的活性; 表达上述多肽的细胞和人CD4蛋白; 以及筛选艾滋病起病抑制剂或HIV-1感染抑制剂的方法,其特征在于使用上述细胞。 根据本发明,可以提供新的鼠CXC趋化因子受体基因,筛选HIV-1感染抑制剂的方法等,其可用于AIDS治疗剂的研究和功能机制 的HIV-1感染。
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公开(公告)号:US20060211037A1
公开(公告)日:2006-09-21
申请号:US11437734
申请日:2006-05-22
申请人: Tadamitsu Kishimoto , Takashi Nagasawa , Kazunobu Tachibana , Hisashi Iizasa , Nobuaki Yoshida , Toshihiro Nakajima , Osamu Yoshie
发明人: Tadamitsu Kishimoto , Takashi Nagasawa , Kazunobu Tachibana , Hisashi Iizasa , Nobuaki Yoshida , Toshihiro Nakajima , Osamu Yoshie
CPC分类号: C07K14/7158 , A61K38/00
摘要: The present invention pertains to a DNA encoding a polypeptide including an entire sequence of the amino acid sequence as shown by SEQ ID NO: 2 or a partial sequence thereof, or a polypeptide including the polypeptide described above, wherein any of the polypeptides has an activity of a receptor capable of binding to a murine PBSF/SDF-1; a polypeptide encoded by the DNA described above, wherein the polypeptide has an activity of a receptor capable of binding to a murine PBSF/SDF-1; cells expressing the polypeptide described above and a human CD4 protein; and a method of screening an AIDS onset inhibitor or an HIV-1 infection inhibitor, characterized by the use of the cells described above. According to the present invention, there can be provided a novel murine CXC chemokine receptor gene, a method of screening an HIV-1 infection inhibitor, and the like, each of which is useful in studies of a therapeutic agent for AIDS and the functional mechanism of HIV-1 infection.
摘要翻译: 本发明涉及编码包含如SEQ ID NO:2或其部分序列所示的氨基酸序列的整个序列或包含上述多肽的多肽的多肽的DNA,其中任何多肽具有活性 的能够结合小鼠PBSF / SDF-1的受体; 由上述DNA编码的多肽,其中所述多肽具有能够结合小鼠PBSF / SDF-1的受体的活性; 表达上述多肽的细胞和人CD4蛋白; 以及筛选艾滋病起病抑制剂或HIV-1感染抑制剂的方法,其特征在于使用上述细胞。 根据本发明,可以提供新的鼠CXC趋化因子受体基因,筛选HIV-1感染抑制剂的方法等,其可用于AIDS治疗剂的研究和功能机制 的HIV-1感染。
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公开(公告)号:US07316926B2
公开(公告)日:2008-01-08
申请号:US10204087
申请日:2001-02-14
CPC分类号: G01N33/5044 , A01K2217/05 , C07K14/005 , C12N5/069 , C12N2503/02 , C12N2510/00 , C12N2510/04 , C12N2517/02 , C12N2710/22022 , G01N33/5008 , G01N33/5011 , G01N33/5017 , G01N33/502 , G01N2500/00
摘要: To provide an immortalized capillary pericyte line which maintains the original function/property of the cell line-deriving tissue, its establishment method, and the screening method for useful substance using the immortalized capillary pericyte line. Cerebral tissue of a transgenic rat carrying the large T antigen gene of SV40 thermo-sensitive mutant line tsA58 is homogenized and the resultant brain capillaries are treated with protease, thus obtained brain capillary cells are subcultured to establish an immortalized cell that expresses SV40 thermo-sensitive large T antigen, PDGF receptor β, and Angiopoietin-1. In addition, the immortalized vascular pericyte line has ability to deposit calcium on matrix by dense culture.
摘要翻译: 提供永生化的毛细管周细胞系,其维持细胞系衍生组织的原始功能/性质,其建立方法和使用永生化毛细管周细胞线的有用物质的筛选方法。 携带SV40热敏突变体株系tsA58的大T抗原基因的转基因大鼠的脑组织被匀化,并用蛋白酶处理所得的脑毛细血管,由此获得的脑毛细血管细胞被传代培养以形成表达SV40热敏感的永生化细胞 大T抗原,PDGF受体β和促血管生成素-1。 此外,永生化的血管周细胞系具有通过致密培养将钙沉积在基质上的能力。
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