公开(公告)号：US06306603B1

公开(公告)日：2001-10-23

申请号：US09476124

申请日：2000-01-03

申请人： Takanori Oka ,  Akio Yamane ,  Takao Tanaka

发明人： Takanori Oka ,  Akio Yamane ,  Takao Tanaka

IPC分类号： C07H2104

CPC分类号： C07K14/70596 ,  C12Q1/6883 ,  C12Q2600/156

摘要： The present invention provides CD36 mutant gene and methods and kits for diagnosing diseases caused by a lipid metabolism abnormality.

摘要翻译： 本发明提供CD36突变基因以及用于诊断由脂质代谢异常引起的疾病的方法和试剂盒。

公开(公告)号：US5601976A

公开(公告)日：1997-02-11

申请号：US945573

申请日：1992-09-16

申请人： Akio Yamane ,  Takanori Oka ,  Satoru Nakagami ,  Kenichi Miyoshi

发明人： Akio Yamane ,  Takanori Oka ,  Satoru Nakagami ,  Kenichi Miyoshi

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C07H21/04 ,  C12N15/00 ,  C12P19/34

CPC分类号： C12Q1/6846 ,  C12Q1/6832

摘要： An intended nucleic acid is determined in a sample by a method in which at least one of the nucleic acid strands of the intended nucleic acid is subjected to hybridization with a primer and to chain-extension reaction over the primer to form a synthesized nucleic acid which is complementary to and in hybridization with the strand; a copy of the intended nucleic acid is obtained, which copy is (a) the nucleic acid of the double-stranded double produced by the chain-extension reaction, or (b) the synthesized nucleic acid freed from the double-stranded nucleic acid, or (c) a double stranded nucleic acid form from a pair of the synthesized nucleic acids complementary with each other; and it is determined whether the copy is present in the sample thereby to know whether the intended nucleic acid is in present in the sample.

摘要翻译： 在样品中通过以下方法测定预期的核酸，其中将使目的核酸的核酸链中的至少一个与引物杂交并通过引物进行链延伸反应以形成合成的核酸，其中 与链互补和杂交; 得到目的核酸的拷贝，其拷贝是（a）通过扩链反应产生的双链双链的核酸，或（b）从双链核酸释放的合成的核酸， 或（c）来自一对互补互补的合成核酸的双链核酸形式; 并确定样品中是否存在拷贝，从而知道目的核酸是否存在于样品中。

公开(公告)号：US5948618A

公开(公告)日：1999-09-07

申请号：US875377

申请日：1997-11-24

申请人： Takanori Oka ,  Akio Yamane

发明人： Takanori Oka ,  Akio Yamane

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

CPC分类号： C12Q1/6827

摘要： A nucleic acid differentiation method utilizing complementary strand-exchange reaction in competitive hybridization can clearly differentiate a site of gene mutation or a difference between genes in a target nucleic acid even when the gene mutation or difference is located near the primer binding site of the target nucleic acid.The invention provides a method and assay kit for differentiating the identity between two nucleic acids by labeling a gene amplifying primer including a primer main region which is complementary to the target nucleic acid to be amplified and having added to the 5' end of the primer main region a sequence which is noncomplementary to the target nucleic acid; amplifying one of two nucleic acids to be determined for identity using the labeled primer, thereby obtaining a labeled DNA; mixing the labeled DNA with an unlabeled DNA prepared from the other nucleic acid to effect competitive hybridization; and measuring the degree of complementary strand-exchange that has occurred between the labeled DNA and the unlabeled DNA by utilizing the label.

摘要翻译： PCT No.PCT / JP96 / 00151 Sec。 371日期：1997年11月24日 102（e）1997年11月24日PCT PCT 1996年1月26日PCT公布。 第WO96 / 23077号公报 日期1996年8月1日在竞争性杂交中使用互补链交换反应的核酸分化方法可以清楚地区分靶核酸中的基因突变位点或基因之间的差异，即使基因突变或差异位于引物结合附近 靶核酸的位点。 本发明提供了一种方法和测定试剂盒，用于通过标记包括与待扩增的靶核酸互补的引物主要区域并已加入引物主体的5'末端的基因扩增引物来区分两个核酸之间的同一性 区域是与靶核酸不互补的序列; 使用标记的引物扩增要鉴定的两种核酸之一，从而获得标记的DNA; 将标记的DNA与从其他核酸制备的未标记的DNA混合以进行竞争性杂交; 并通过利用标记物测量标记的DNA与未标记的DNA之间发生的互补链交换的程度。

公开(公告)号：US5688643A

公开(公告)日：1997-11-18

申请号：US392818

申请日：1995-02-27

申请人： Takanori Oka ,  Hironari Matsunaga ,  Akio Yamane

发明人： Takanori Oka ,  Hironari Matsunaga ,  Akio Yamane

IPC分类号： C12Q1/68 ,  C12N15/00

CPC分类号： C12Q1/6827

摘要： A nucleic acid differentiating method is provided which involves using a primer having a detectable label introduced therein and a primer having a solid matrix-binding site introduced therein, amplifying a particular region of a target nucleic acid in a sample to thereby produce a labeled sample DNA, adding to this labeled sample DNA at least an equimolar amount of an unlabeled standard DNA to be evaluated for its sequence matching with the sample DNA, effecting competitive hybridization, and at the end of reaction, determining the label intensity of the hybridization product, for thereby determining the presence/absence of a mutant gene in the nucleic acid, the ratio of normal to mutant genes, or the sequence matching of a particular gene among a plurality of samples. An assay kit for differentiating nucleic acid by the differentiating method is also provided which includes target nucleic acid-amplifying primers comprising a primer having a detectable label introduced therein and a primer having a solid matrix-binding site introduced therein and an unlabeled DNA specimen to be evaluated for its matching with the amplified product amplified with the primers.

摘要翻译： PCT No.PCT / JP94 / 01106 Sec。 371日期：1995年2月27日 102（e）1995年2月27日PCT PCT 1994年7月7日PCT公布。 WO95 / 02068 PCT出版物 日本1995年1月19日提供了一种核酸分化方法，其涉及使用引入其中具有可检测标记的引物和引入其中的固体基质结合位点的引物，扩增样品中靶核酸的特定区域，从而 产生标记的样品DNA，将至少等摩尔量的未标记的标准DNA加入到标记的样品DNA中，以评估其与样品DNA的序列匹配，进行竞争性杂交，并且在反应结束时，确定标记样品DNA的标记强度 杂交产物，从而确定核酸中突变基因的存在/不存在，正常与突变基因的比例，或多个样品中特定基因的序列匹配。 还提供了用于通过分化方法分化核酸的测定试剂盒，其包括靶引物，其包含引入其中具有可检测标记的引物和引入其中的固体基质结合位点的引物和未标记的DNA标本 评估其与用引物扩增的扩增产物的匹配。

公开(公告)号：US5705159A

公开(公告)日：1998-01-06

申请号：US190801

申请日：1994-02-01

申请人： Reiko F. Irie ,  Takanori Oka

发明人： Reiko F. Irie ,  Takanori Oka

IPC分类号： C12N15/02 ,  A61K35/26 ,  A61K38/00 ,  A61K38/45 ,  A61K39/00 ,  A61K39/395 ,  A61P35/00 ,  A61P37/04 ,  C07H21/04 ,  C07K14/47 ,  C07K16/30 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/04 ,  C12P21/08 ,  C12R1/19 ,  C12R1/91 ,  C07K7/00 ,  C07K7/06

CPC分类号： C07K14/4748 ,  C07K14/47 ,  C07K16/3053 ,  A61K2039/505 ,  A61K38/00 ,  A61K39/00 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2319/00

摘要： A tumor associated protein has been discovered to exhibit immunoreactivity to human monoclonal antibody L92. A ten amino acid peptide segment of the protein and a fourteen amino acid peptide which contains the ten amino acid peptide has also been isolated and exhibits immunoreactivity to the same antibody. Using truncated fusion proteins, the minimum recognition site for antibody binding was determined to be four amino acids. Also disclosed are polypeptide compositions against human tumors that includes the polypeptides or protein of the present disclosure, as well as antibodies reactive with these polypeptides that may be employed directly for treatment or diagnosis. A certain embodiment of the present invention is also the DNA sequence encoding the ten amino acid peptide and the DNA encoding the fourteen amino acid peptide which also exhibits immunoreactivity to human monoclonal antibody L92 and cytotoxic T cells.

摘要翻译： 已经发现肿瘤相关蛋白对人单克隆抗体L92表现出免疫反应性。 蛋白质的十个氨基酸肽段和含有十个氨基酸肽的十四个氨基酸肽也已被分离，并显示出对相同抗体的免疫反应性。 使用截短的融合蛋白，测定抗体结合的最小识别位点为四个氨基酸。 还公开了包含本公开的多肽或蛋白质的人肿瘤的多肽组合物，以及可直接用于治疗或诊断的与这些多肽反应的抗体。 本发明的某个实施方案也是编码十个氨基酸肽的DNA序列和编码与人单克隆抗体L92和细胞毒性T细胞具有免疫反应性的十四个氨基酸肽的DNA。

公开(公告)号：US06288035B1

公开(公告)日：2001-09-11

申请号：US09504410

申请日：2000-02-15

申请人： Reiko F. Irie ,  Takanori Oka

发明人： Reiko F. Irie ,  Takanori Oka

IPC分类号： A61K3800

CPC分类号： C07K14/4748 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  C07K14/47 ,  C07K16/3053 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2319/00

摘要： A tumor associated protein has been discovered to exhibit immunoreactivity to human monoclonal antibody L92. A ten amino acid peptide segment of the protein and a fourteen amino acid peptide which contains the ten amino acid peptide has also been isolated and exhibits immunoreactivity to the same antibody. Using truncated fusion proteins, the minimum recognition site for antibody binding was determined to be four amino acids. Also disclosed are polypeptide compositions against human tumors that includes the polypeptides or protein of the present disclosure, as well as antibodies reactive with these polypeptides that may be employed directly for treatment or diagnosis. A certain embodiment of the present invention is also the DNA sequence encoding the ten amino acid peptide and the DNA encoding the fourteen amino acid peptide which also exhibits immunoreactivity to human monoclonal antibody L92 and cytotoxic T cells.

摘要翻译： 已经发现肿瘤相关蛋白对人单克隆抗体L92表现出免疫反应性。 蛋白质的十个氨基酸肽段和含有十个氨基酸肽的十四个氨基酸肽也已被分离，并显示出对相同抗体的免疫反应性。 使用截短的融合蛋白，测定抗体结合的最小识别位点为四个氨基酸。 还公开了包含本公开的多肽或蛋白质的人肿瘤的多肽组合物，以及可直接用于治疗或诊断的与这些多肽反应的抗体。 本发明的某个实施方案也是编码十个氨基酸肽的DNA序列和编码与人单克隆抗体L92和细胞毒性T细胞具有免疫反应性的十四个氨基酸肽的DNA。

公开(公告)号：US6077950A

公开(公告)日：2000-06-20

申请号：US247146

申请日：1999-02-09

申请人： Reiko F. Irie ,  Takanori Oka

发明人： Reiko F. Irie ,  Takanori Oka

IPC分类号： C12N15/02 ,  A61K35/26 ,  A61K38/00 ,  A61K38/45 ,  A61K39/00 ,  A61K39/395 ,  A61P35/00 ,  A61P37/04 ,  C07H21/04 ,  C07K14/47 ,  C07K16/30 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/04 ,  C12P21/08 ,  C12R1/19 ,  C12R1/91 ,  A61K38/04

CPC分类号： C07K14/4748 ,  C07K14/47 ,  C07K16/3053 ,  A61K2039/505 ,  A61K38/00 ,  A61K39/00 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2319/00

摘要： A tumor associated protein has been discovered to exhibit immunoreactivity to human monoclonal antibody L92. A ten amino acid peptide segment of the protein and a fourteen amino acid peptide which contains the ten amino acid peptide has also been isolated and exhibits immunoreactivity to the same antibody. Using truncated fusion proteins, the minimum recognition site for antibody binding was determined to be four amino acids. Also disclosed are polypeptide compositions against human tumors that includes the polypeptides or protein of the present disclosure, as well as antibodies reactive with these polypeptides that may be employed directly for treatment or diagnosis. A certain embodiment of the present invention is also the DNA sequence encoding the ten amino acid peptide and the DNA encoding the fourteen amino acid peptide which also exhibits immunoreactivity to human monoclonal antibody L92 and cytotoxic T cells.

摘要翻译： 已经发现肿瘤相关蛋白对人单克隆抗体L92表现出免疫反应性。 蛋白质的十个氨基酸肽段和含有十个氨基酸肽的十四个氨基酸肽也已被分离，并显示出对相同抗体的免疫反应性。 使用截短的融合蛋白，测定抗体结合的最小识别位点为四个氨基酸。 还公开了包含本公开的多肽或蛋白质的人肿瘤的多肽组合物，以及可直接用于治疗或诊断的与这些多肽反应的抗体。 本发明的某个实施方案也是编码十个氨基酸肽的DNA序列和编码与人单克隆抗体L92和细胞毒性T细胞具有免疫反应性的十四个氨基酸肽的DNA。

公开(公告)号：US09079961B2

公开(公告)日：2015-07-14

申请号：US12451655

申请日：2008-05-26

申请人： Atsuko Taniue ,  Hiroyuki Ishii ,  Shinji Maekawajiri ,  Nozomi Nagata ,  Takanori Oka

发明人： Atsuko Taniue ,  Hiroyuki Ishii ,  Shinji Maekawajiri ,  Nozomi Nagata ,  Takanori Oka

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C07K16/28 ,  C07K14/705 ,  G01N33/86

CPC分类号： C07K16/2848 ,  C07K14/70557 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01N33/86 ,  G01N2800/222 ,  G01N2800/245

摘要： An object of the present invention is to provide a probe, primer, primer set and antibody for determining neonatal alloimmune thrombocytopenic purpura or the risk of developing it. According to the present invention, there is provided a probe, primer, primer set and antibody for use in the detection of the thymine residue at position 1297 in the GPIIIa.

摘要翻译： 本发明的目的是提供用于确定新生儿同种异体免疫性血小板减少性紫癜的探针，引物，引物组和抗体或其发展的风险。 根据本发明，提供了用于检测GPIIIa中1297位的胸腺嘧啶残基的探针，引物，引物组和抗体。

公开(公告)号：US20130335194A1

公开(公告)日：2013-12-19

申请号：US14000885

申请日：2012-01-05

申请人： Ryoji Shimizu ,  Masashi Sato ,  Hiroaki Takeya ,  Takanori Oka ,  Mitsunori Sugiura

发明人： Ryoji Shimizu ,  Masashi Sato ,  Hiroaki Takeya ,  Takanori Oka ,  Mitsunori Sugiura

IPC分类号： G07C9/00

CPC分类号： G07C9/00896

摘要： A key managed by a key management device has an operation detecting unit configured to detect an operating state thereof, and a transmitter configured to transmit information of the operating state detected by the operation detecting unit to receiving devices through wireless communication. The operation detecting unit includes an acceleration sensor, and detects information of acceleration measured by the acceleration sensor as the operating state thereof.

摘要翻译： 由密钥管理装置管理的密钥具有检测其操作状态的操作检测单元和被配置为通过无线通信将由操作检测单元检测到的操作状态的信息发送给接收设备的发送器。 操作检测单元包括加速度传感器，并且检测由加速度传感器测量的加速度信息作为其运行状态。

公开(公告)号：US09261285B2

公开(公告)日：2016-02-16

申请号：US12581714

申请日：2009-10-19

申请人： Michio Setsu ,  Takanori Oka ,  Hirokazu Uemae ,  Keisuke Uchida

发明人： Michio Setsu ,  Takanori Oka ,  Hirokazu Uemae ,  Keisuke Uchida

IPC分类号： F25B29/00 ,  F28D1/00 ,  F24F1/02 ,  F24F13/30

CPC分类号： F24F1/027 ,  F24F13/30

摘要： An air conditioner has: a casing 20 having an air inlet 21 and an air outlet 22; a heater 53 disposed in the casing 20 and heating air having flowed through the air inlet 21 into the casing 20; holders 54 and 55 holding the heater 53 and fitted to the casing 20; and a spacer 59 in contact with the heater 53 and disposed between the holders 54 and 55 and the heater 53. At least one of the heat-resistance and the flame-retardance of the spacer 59 is higher than that of the holders 54 and 55.

摘要翻译： 空调器具有：具有空气入口21和空气出口22的壳体20; 设置在壳体20中的加热器53，并且通过空气入口21流入壳体20的加热空气; 夹持加热器53并装配到壳体20的保持器54和55; 以及与加热器53接触并且设置在保持件54和55与加热器53之间的间隔件59.间隔件59的耐热性和阻燃性中的至少一个高于保持件54和55的耐热性和阻燃性 。