利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

32 条记录 (耗时 0.02 秒)

    Reagent for classifying leukocytes by flow cytometry
    1.
    发明授权
    Reagent for classifying leukocytes by flow cytometry 失效
    通过流式细胞术分类白细胞的试剂

    公开(公告)号：US5175109A

    公开(公告)日：1992-12-29

    申请号：US663090

    申请日：1991-02-28

    申请人： Takashi Sakata ,  Tomoyuki Kuroda

    发明人： Takashi Sakata ,  Tomoyuki Kuroda

    IPC分类号： G01N15/14 ,  G01N33/50

    CPC分类号： G01N33/5094 ,  G01N15/1456 ,  Y10S436/80 ,  Y10T436/107497

    摘要： A reagent for classifying leukocytes with a flow cytometer by means of optical measurements on fluorochrome-stained blood cells is included. The reagent is useful in the practice of clinical testing and includes a Neutral Red fluorochrome and either an Astrazon Organe G or an Auramine O fluorochrome to enable differentiation of leukocytes.

    摘要翻译： 包括通过荧光染色血细胞上的光学测量用流式细胞仪分类白细胞的试剂。 该试剂在临床试验的实践中是有用的，并且包括中性红色荧光染料和Astrazon Organe G或Auramine O荧光染料以使白细胞分化。

    Method for classifying leukocytes by flow cytometry
    5.
    发明授权
    Method for classifying leukocytes by flow cytometry 失效
    流式细胞仪分类白细胞的方法

    公开(公告)号：US5296378A

    公开(公告)日：1994-03-22

    申请号：US947784

    申请日：1992-09-18

    申请人： Takashi Sakata ,  Tomoyuki Kuroda

    发明人： Takashi Sakata ,  Tomoyuki Kuroda

    IPC分类号： G01N15/14 ,  G01N33/50 ,  G01N33/49

    CPC分类号： G01N33/5094 ,  G01N15/1456 ,  Y10S436/80 ,  Y10T436/107497

    摘要： A method for classifying leukocytes with a flow cytometer by use of optical measurements on fluorochrome-stained blood cells. The method comprises the steps of lysing erythrocytes in a blood sample, selectively staining eosinophils and basophils, permitting stained sample to flow through a flow cytometer, and identifying and counting the type of each leukocyte.

    摘要翻译： 使用荧光染料染色的血细胞上的光学测量法，用流式细胞仪分类白细胞的方法。 该方法包括以下步骤：在血液样品中裂解红细胞，选择性染色嗜酸性粒细胞和嗜碱性粒细胞，允许染色的样品流过流式细胞仪，以及鉴定和计数每种白细胞的类型。

    Process for discriminating and counting erythroblasts
    7.
    发明授权
    Process for discriminating and counting erythroblasts 失效
    红细胞鉴别和计数的方法

    公开(公告)号：US06911313B2

    公开(公告)日：2005-06-28

    申请号：US09058323

    申请日：1998-04-09

    申请人： Berend Houwen ,  Fu-sheng Wang ,  Tomohiro Tsuji ,  Takashi Sakata ,  Yukio Hamaguchi

    发明人： Berend Houwen ,  Fu-sheng Wang ,  Tomohiro Tsuji ,  Takashi Sakata ,  Yukio Hamaguchi

    IPC分类号： G01N33/50 ,  G01N33/569 ,  G01N33/80 ,  G01N33/53 ,  G01N21/76

    CPC分类号： G01N33/5094 ,  G01N15/1459 ,  G01N33/56972 ,  G01N33/80 ,  G01N2015/1006 ,  G01N2015/1402 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/13 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375

    摘要： A method for discriminating and counting erythroblasts comprises the steps of: (i) staining leukocytes in a hematologic sample by adding a fluorescent labeled antibody capable of binding specifically with leukocytes to the hematologic sample; (ii) raising the permeability only of cell membranes of erythroblasts in the hematologic sample to a nucleotide fluorescent dye which does not permeate a cell membrane usually, the nucleotide fluorescent dye having a fluorescent spectrum capable of being distinguished from that of a fluorescent labeling compound of the fluorescent labeled antibody in step (i); (iii) staining nuclei of the erythroblasts in the hematologic sample with the nucleotide fluorescent dye; (iv) subjecting the hematologic sample to flowcytometry to detect at least two fluorescent signals from each cell; and (v) discriminating and counting the erythroblasts from difference in intensity between the at least two fluorescent signals.

    摘要翻译： 用于鉴别和计数红细胞的方法包括以下步骤：（i）通过将能够与白细胞特异性结合的荧光标记抗体加入到血液样品中来染色血液学样品中的白细胞; （ii）将血液学样品中的红细胞的细胞膜的渗透性提高至不透过细胞膜的核苷酸荧光染料，所述核苷酸荧光染料具有与荧光标记化合物的荧光标记化合物不同的荧光光谱 步骤（i）中的荧光标记抗体; （iii）用核苷酸荧光染料染色血液样品中的红细胞核; （iv）使血液样品经流式细胞术检测来自每个细胞的至少两个荧光信号; 和（v）鉴别和计数红细胞与至少两个荧光信号之间的强度差异。

    Method for classifying and counting immature leukocytes
    8.
    发明授权
    Method for classifying and counting immature leukocytes 失效
    不成熟白细胞分类和计数方法

    公开(公告)号：US5958776A

    公开(公告)日：1999-09-28

    申请号：US972103

    申请日：1997-11-17

    申请人： Takashi Sakata ,  Toshihiro Mizukami ,  Kayo Hatanaka

    发明人： Takashi Sakata ,  Toshihiro Mizukami ,  Kayo Hatanaka

    IPC分类号： G01N33/48 ,  G01N15/14 ,  G01N33/49 ,  G01N33/50 ,  G01N31/00

    CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

    摘要： A hematological sample is treated with a hemolytic agent that maintains immature leukocytes in a viable state and damages other leukocytes. The damaged leukocytes are stained with a fluorochrome which can stain damaged cells. At least one kind of scattered light and at least one kind of fluorescence of the treated and stained blood cells are measured to classify and count leukocytes based on the intensities of the scattered light and the fluorescence. Highly precise measurement of immature leukocytes and simultaneous classification and counting of normal leukocytes are achieved.

    摘要翻译： 用溶血剂处理血液样品，其将未成熟白细胞维持在可行状态并损伤其他白细胞。 损伤的白细胞用可染色受损细胞的荧光染料染色。 根据散射光和荧光的强度，测量处理和染色的血细胞中的至少一种散射光和至少一种荧光，以对白细胞进行分类和计数。 实现了对未成熟白细胞的高精度测量，并实现了正常白细胞的同时分类和计数。

    Reagent for measurement of leukocytes and hemoglobin in blood
    10.
    发明授权
    Reagent for measurement of leukocytes and hemoglobin in blood 失效
    血液中白细胞介素和血红蛋白测定试剂

    公开(公告)号：US5242832A

    公开(公告)日：1993-09-07

    申请号：US596205

    申请日：1990-10-10

    申请人： Takashi Sakata

    发明人： Takashi Sakata

    IPC分类号： G01N33/50 ,  G01N33/72

    CPC分类号： G01N33/5094 ,  G01N33/721 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/2525

    摘要： A reagent, satisfying the undermentioned conditions, is useful for counting the number of leukocytes and measuring the hemoglobin concentration in blood samples. The reagent is free of cyanides and it is able to maintain hemoglobin in a stable state for a prolonged time. The reagent contains at least one cationic surfactant selected from the group consisting of a quaternary ammonium salt of a concentration capable of hemolyzing erythrocytes in the blood and oxidizing hemoglobin and a composition of pyridinium salts; at least one cationic, nonionic or amphoteric surfactant; and at least one hemoglobin stabilizer.