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    Method for classifying and counting leukocytes
    1.
    发明授权
    Method for classifying and counting leukocytes 失效
    白细胞分类和计数方法

    公开(公告)号：US5677183A

    公开(公告)日：1997-10-14

    申请号：US464056

    申请日：1995-06-05

    申请人： Kaoru Takarada ,  Chihiro Kouzuki ,  Yoshihiro Hyousa ,  Takashi Sakata ,  Yasumasa Akai

    发明人： Kaoru Takarada ,  Chihiro Kouzuki ,  Yoshihiro Hyousa ,  Takashi Sakata ,  Yasumasa Akai

    IPC分类号： G01B11/02 ,  A61K35/14 ,  G01N15/14 ,  G01N33/49 ,  G01N33/50 ,  G01N31/00 ,  G01N33/48

    CPC分类号： G01N33/5094 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25125

    摘要： A method for classifying and counting leukocytes which includes the steps of (i) adding a first reagent used for classifying leukocytes into four groups which contains, (a) at least one ionic surfactant in a sufficient amount to lyse erythrocytes and to damage a part of cell membrane of leukocytes, (b) at least one organic compound having an anionic group in a sufficient amount to bond with a cationic component present in leukocytes to give morphological differences between leukocytes, (c) a nonionic surfactant, and (d) a buffer for adjusting pH, to a part of a blood sample to determine information on the cell size and morphological features in order to classify leukocytes into four groups consisting of three groups corresponding to lymphocytes, mononuclear cells and eosinophils and one group corresponding to neutrophils and basophils; (ii) adding a second reagent used for measuring basophils to another part of the blood sample to determine information on at least the cell size in order to classify basophils, and (iii) classifying leukocytes based on the information obtained in the steps (i) and (ii) and counting.

    摘要翻译： 一种白细胞分类和计数方法，包括以下步骤：（i）将用于将白细胞分类的第一试剂加入到四个组中，所述四组包含（a）足够量的至少一种离子表面活性剂以溶解红细胞并损伤一部分 白细胞的细胞膜，（b）至少一种具有足够量的具有阴离子基团的有机化合物与白细胞中存在的阳离子成分结合以产生白细胞之间的形态学差异，（c）非离子表面活性剂和（d）缓冲液 用于调节pH至血液样品的一部分以确定关于细胞大小和形态特征的信息，以将白细胞分类为由对应于淋巴细胞，单核细胞和嗜酸性粒细胞的三组组成的四组，以及对应于嗜中性粒细胞和嗜碱性粒细胞的一组。 （ii）将用于测量嗜碱性粒细胞的第二试剂加入到血液样品的另一部分中以确定关于至少细胞大小的信息以便分解嗜碱性粒细胞，和（iii）基于步骤（i）中获得的信息对白细胞进行分类， 和（ii）并计数。

    Particle analyzer
    3.
    发明授权
    Particle analyzer 失效
    粒子分析仪

    公开(公告)号：US5506673A

    公开(公告)日：1996-04-09

    申请号：US332040

    申请日：1994-11-01

    申请人： Tokihiro Kosaka ,  Kaoru Takarada

    发明人： Tokihiro Kosaka ,  Kaoru Takarada

    IPC分类号： G01N15/10 ,  G01N15/12 ,  G01N15/14

    CPC分类号： G01N15/14 ,  G01N15/12 ,  G01N15/1012 ,  G01N2015/1037

    摘要： A particle analyzer includes a flow cell for enclosing an electrolyte flow containing particles in a sheathed flow, the flow cell including a first and second cells which are interconnected through an orifice, the sheathed flow flowing through the orifice from the first cell to the second cell, a first and second electrodes provided in the first and second cells, respectively and a voltage application device for applying voltage between the first and second electrodes. The particle analyzer further includes a resistance detection device for detecting a variation of electric resistance between the first and second electrodes when the particle passes through the orifice to output the detected variation as a resistance detection signal. A first diameter calculation device is included for calculating each diameter of the particles based on the detected variation of electric resistance. Further a light source device for emitting a light beam to the particles enclosed in the sheathed flow and a light detection device for detecting each intensity of scatter light of the particles irradiated with the light beam to output the detected intensity as a scatter light detection signal are included. Finally, the particle analyzer includes a refractive index calculation device for calculating a refractive index of the particle based on the resistance detection signal and the scatter light detection signal and a second diameter calculation device for calculating each diameter of the particles based on the calculated refractive index and the scatter light detection signal.

    摘要翻译： 颗粒分析仪包括用于封闭在护套流中含有颗粒的电解质流的流动池，所述流动池包括通过孔相互连接的第一和第二电池，所述护套流动通过所述孔从所述第一电池流到所述第二电池 分别设置在第一和第二单元中的第一和第二电极以及用于在第一和第二电极之间施加电压的电压施加装置。 粒子分析仪还包括电阻检测装置，用于当粒子通过孔口时检测第一和第二电极之间的电阻变化，以输出检测到的变化作为电阻检测信号。 包括第一直径计算装置，用于基于检测到的电阻变化来计算颗粒的每个直径。 另外，用于将光束发射到封装在护套流中的颗粒的光源装置和用于检测被光束照射的颗粒的散射光的每个强度的光检测装置，以输出检测的强度作为散射光检测信号， 包括。 最后，粒子分析仪包括用于基于电阻检测信号和散射光检测信号计算粒子的折射率的折射率计算装置和用于基于计算出的折射率计算颗粒的每个直径的第二直径计算装置 和散射光检测信号。