.beta.-galactoside-.alpha.-2, 6-sialyltransferase, and a process for
producing from Photobacterium
    1.
    发明授权
    .beta.-galactoside-.alpha.-2, 6-sialyltransferase, and a process for producing from Photobacterium 失效
    β-半乳糖苷-α-2,6-唾液酸转移酶和由光杆菌生产的方法

    公开(公告)号:US5827714A

    公开(公告)日:1998-10-27

    申请号:US739015

    申请日:1996-10-28

    IPC分类号: C12N9/10 C12N1/20

    CPC分类号: C12N9/1081 Y10S435/822

    摘要: A .beta.-galactoside-.alpha.-2,6-sialyltransferase derived from a microorganism belonging to the genus Photobacterium has ben disclosed, having the following physicochemical properties: (1) action and specificity: transferring sialic acid from cytidine monophosphate-sialic acid to the 6-position of a galactose residue in a sugar chain of a glycoconjugate or in a free sugar chain, or to the 6-position of a monosaccharide having a hydroxyl group on carbon at the 6-position and being capable of forming a glycoconjugate. (2) optimum pH: 5 to 6; (3) optimum temperature: 30.degree. C.; and (4) molecular weight: 64,000.+-.5,000 (determined by gel filtration).

    摘要翻译: 衍生自属于光杆菌属的微生物的β-半乳糖苷-α-2,6-唾液酸转移酶已公开,具有以下物理化学性质:(1)作用和特异性:将唾液酸从胞苷一磷酸唾液酸转移至6 在糖缀合物或游离糖链的糖链中的半乳糖残基的位置,或6-位上具有羟基的单糖的6-位,并且能够形成糖缀合物。 (2)最适pH:5〜6; (3)最适温度:30℃。 和(4)分子量:64,000 +/- 5,000(通过凝胶过滤测定)。

    &bgr;-galactoside-&agr;2, 6-sialyltransferase gene
    3.
    发明授权
    &bgr;-galactoside-&agr;2, 6-sialyltransferase gene 有权
    β-半乳糖苷-α2,6-唾液酸转移酶基因

    公开(公告)号:US06255094B1

    公开(公告)日:2001-07-03

    申请号:US09171878

    申请日:1999-01-05

    IPC分类号: C12N910

    CPC分类号: C12N9/1081 C12N9/1077

    摘要: This invention provides to a novel gene encoding a protein aving the activity of &bgr;-galactoside-&agr;2,6-sialyltransferase. The gene of the invention encodes a protein which having the amino acid sequence of SEQ ID NO:1, or a protein having the amino acid sequence of SEQ ID NO:1 which have been modified by deletion, substitution or addition of at least one amino acid residue in said sequence, while maintaining substantially the same &bgr;-galactoside-&agr;2,6-sialyltransferase activity. The present invention uses said gene to further provide a vector for expressing a protein having the &bgr;-galactoside-&agr;2,6-sialyltransferase activity, host cells and a recombinant protein. The protein encoded by the gene of the invention does not have any substantial homology with known sialyltransferases, and in addition, the membrane binding region is located in the C-terminal unlike known sialyltransferases.

    摘要翻译: 本发明提供了一种新型的编码β-半乳糖苷-α2,6-唾液酸转移酶活性蛋白质的基因。本发明的基因编码具有SEQ ID NO:1的氨基酸序列的蛋白质,或具有 通过在所述序列中缺失,取代或添加至少一个氨基酸残基而修饰的SEQ ID NO:1的氨基酸序列,同时保持基本上相同的β-半乳糖苷-α2,6-唾液酸转移酶活性。本发明使用 所述基因进一步提供用于表达具有β-半乳糖苷-α2,6-唾液酸转移酶活性的蛋白质的载体,宿主细胞和重组蛋白。 由本发明的基因编码的蛋白质与已知的唾液酸转移酶不具有任何实质的同源性,此外,与已知的唾液酸转移酶不同,膜结合区位于C末端。

    .alpha.-agarase and production process of oligosaccharides and
monosaccharides
    4.
    发明授权
    .alpha.-agarase and production process of oligosaccharides and monosaccharides 失效
    α-糖基化酶和寡糖和单糖的生产过程

    公开(公告)号:US5834257A

    公开(公告)日:1998-11-10

    申请号:US554591

    申请日:1995-11-06

    摘要: The present inventions relate to novel .alpha.-agarase which selectively hydrolyzes the 1,3-bond of an oligosaccharide derived from agarose; a process for producing oligosaccharides and/or monosaccharides, characterized by that a .beta.-agarase which acts on both polysaccharides and oligosaccharides derived from agarose and an .alpha.-agarase which acts specifically on oligosaccharides derived from agarose are allowed to react with a substrate which contains agarose or an oligosaccharide derived from agarose; and a process for producing oligosaccharides and/or monosaccharides, characterized by that the above .alpha.-agarase is allowed to react with a substrate which contains an oligosaccharide derived from agarose.

    摘要翻译: 本发明涉及选自水解衍生自琼脂糖的寡糖的1,3-键的新型α-糖基化酶; 一种生产寡糖和/或单糖的方法,其特征在于,对来自琼脂糖的多糖和寡糖起作用的β-糖基化酶和特异性地作用于源自琼脂糖的寡糖的α-胶原酶被允许与含有琼脂糖的底物 或衍生自琼脂糖的寡糖; 以及制备寡糖和/或单糖的方法,其特征在于使上述α-胶原酶与含有源自琼脂糖的寡糖的底物反应。

    Nonwoven fabric and electrolyte membrane
    5.
    发明授权
    Nonwoven fabric and electrolyte membrane 有权
    无纺布和电解质膜

    公开(公告)号:US08394549B2

    公开(公告)日:2013-03-12

    申请号:US13194241

    申请日:2011-07-29

    摘要: To obtain a nonwoven fabric which is excellent in the heat resistance and the chemical resistance, of which the fiber diameter is small, and which is excellent in the mechanical strength at a temperature at which it is used; and an electrolyte membrane which is excellent in the dimensional stability when it is swollen by water, and of which an increase in the resistance by a reinforcing material is suppressed.A nonwoven fabric 28 containing fibers 26 of an ethylene/tetrafluoroethylene copolymer having a storage elastic modulus E′ at 25° C. of at least 8×108 Pa and a melt viscosity measured at 300° C. of higher than 60 Pa·s and at most 300 Pa·s, wherein the average fiber diameter of the fibers is from 0.01 to 3 μm; and an electrolyte membrane reinforced by the nonwoven fabric 28.

    摘要翻译: 为了得到纤维直径小的耐热性和耐化学性优异的非织造布,其使用温度下的机械强度优异; 以及电解质膜,其由水膨胀时的尺寸稳定性优异,并且抑制了增强材料的电阻的增加。 包含25℃下的储能弹性模量E'至少为8×10 8 Pa,在300℃下测定的熔体粘度高于60Pa·s的乙烯/四氟乙烯共聚物的纤维26的无纺布28和 最多300Pa·s,其中纤维的平均纤维直径为0.01〜3μm; 以及由无纺布28增强的电解质膜。

    Process cartridge, mounting mechanism for process cartridge and electrophotographic image forming apparatus

    公开(公告)号:US06714750B2

    公开(公告)日:2004-03-30

    申请号:US09995603

    申请日:2001-11-29

    IPC分类号: G03G1500

    摘要: A process cartridge detachably mountable to a main assembly of an electrophotographic image forming apparatus which includes an openable closing member, a first main assembly guide movable in interrelation with opening and closing action of the closing member, and a second main assembly guide, the process cartridge include a photosensitive drum; a first cartridge frame portion extending in a cartridge mounting direction to the main assembly, at one axial end portion of the drum; a first cartridge guide projected from the first cartridge frame portion, the first cartridge guide moving the cartridge toward a cartridge mounting position by movement of the first main assembly guide with the cartridge being supported on first main assembly guide, upon mounting of the cartridge to the main assembly; a second cartridge frame portion extended in the mounting direction at the other axial end portion of the drum; a second cartridge guide projected from the second cartridge frame portion, the second cartridge guide moving the cartridge toward a cartridge mounting position by movement of the second main assembly guide with the cartridge being supported on the second main assembly guide; when the cartridge is mounted to the main assembly of the apparatus; a first cartridge positioning portion for engaging with a first main assembly positioning portion provided in the main assembly to position the cartridge relative to the main assembly, the first cartridge positioning portion being projected outwardly from the first cartridge frame portion and coaxially with the drum at the one axial end of the drum; and a second cartridge positioning portion for engaging with a second main assembly positioning portion to position the cartridge relative to the main assembly when the cartridge is mounted to the main assembly, the second cartridge positioning portion being projected outwardly from the second cartridge frame portion and coaxially with the drum at the other axial end of the drum.

    Electrophotographic image forming apparatus, electrophotographic image forming system, and process cartridge
    7.
    发明授权
    Electrophotographic image forming apparatus, electrophotographic image forming system, and process cartridge 有权
    电子照相图像形成装置,电照相成像系统和处理盒

    公开(公告)号:US06201935B1

    公开(公告)日:2001-03-13

    申请号:US09370907

    申请日:1999-08-10

    IPC分类号: G03G1500

    摘要: An electrophotographic image forming apparatus onto which a process cartridge is detachably mountable, for forming an image on a recording medium, has (a) a mounting member for detachably mounting a process cartridge having an electrophotographic photosensitive member, a charging member for charging the electrophotographic photosensitive member, a cartridge charge bias contact for receiving a charging bias to be impressed on the charging member from the main body of the apparatus when the process cartridge is mounted on the main body of the apparatus, and a cartridge-earth contact for connecting the electrophotographic photosensitive member to ground with the main body of the apparatus when the process cartridge is mounted on the main body of the apparatus, (b) a main-body-charge bias contact to be electrically connected to the cartridge-charge-bias contact when the process cartridge is mounted on the mounting member, (c) a main-body earth contact to be electrically connected to the cartridge-earth contact when the process cartridge is mounted on the mounting member, (d) a detecting member for detecting that the process cartridge is not mounted in a cartridge-mounting position by detecting that at least one of the cartridge-charge-bias contact and the cartridge-earth contact is not electrically connected to a corresponding one of the main-body-charge bias contact and the main-body earth contact and (e) a conveying member for conveying the recording medium.

    摘要翻译: 一种电子照相图像形成装置,其上可拆卸地安装处理盒,用于在记录介质上形成图像,具有(a)用于可拆卸地安装具有电子照相感光构件的处理盒的安装构件,用于对电子照相感光体充电的充电构件 当处理盒安装在设备的主体上时,用于从设备的主体接受从充电构件施加的充电偏压的盒式充电偏压触点,以及用于连接电子照相 当处理盒安装在设备的主体上时,感光构件与设备的主体接地,(b)主体充电偏压接触件,当主体充电偏压触点电连接到盒充电偏压触点时 处理盒安装在安装构件上,(c)主体接地触点,以电连接到插座 当处理盒安装在安装构件上时,进行接地间接触,(d)检测构件,用于检测处理盒未安装在盒安装位置,通过检测至少一个盒充电偏置触点 并且盒式接地触头不与主体充电偏压触点和主体接地触头中的相应一个电连接,以及(e)用于传送记录介质的输送构件。

    Anion exchanger
    10.
    发明授权
    Anion exchanger 失效
    ANION交换机

    公开(公告)号:US5180750A

    公开(公告)日:1993-01-19

    申请号:US385229

    申请日:1989-07-26

    IPC分类号: C08G75/23

    CPC分类号: C08G75/23

    摘要: An anion exchanger consisting essentially of an aromatic polysulfone block copolymer of the formula: ##STR1## wherein Ar is ##STR2## or ##STR3## X is --SO.sub.2 --, ##STR4## --S-- or --O--, Y is --SO.sub.2 --, --S-- or --O--, Z is a single bond, --O--, --S--, --SO.sub.2 --, ##STR5## each of R.sub.1 to R.sub.9 which may be the same or different is a hydrocarbon group having from 1 to 8 carbon atoms, each of a to d is an integer of from 0 to 4, e is an integer of from 0 to 3, (f+g) is from 0 to 7, (h+i) is from 0 to 5, each of R.sub.10 and R.sub.11 is a hydrogen atom or a hydrocarbon group having from 1 to 6 carbon atoms, and each of m and n is an integer of from 2 to 200, provided m/n=100/1-1/10, and having anion exchange groups introduced to the aromatic rings of the copolymer.