摘要:
A .beta.-galactoside-.alpha.-2,6-sialyltransferase derived from a microorganism belonging to the genus Photobacterium has ben disclosed, having the following physicochemical properties: (1) action and specificity: transferring sialic acid from cytidine monophosphate-sialic acid to the 6-position of a galactose residue in a sugar chain of a glycoconjugate or in a free sugar chain, or to the 6-position of a monosaccharide having a hydroxyl group on carbon at the 6-position and being capable of forming a glycoconjugate. (2) optimum pH: 5 to 6; (3) optimum temperature: 30.degree. C.; and (4) molecular weight: 64,000.+-.5,000 (determined by gel filtration).
摘要:
Described is a method of production of saccharides containing sialic acid, wherein .beta.-galactoside-.alpha.-2,6-sialyltransferase is used for linking sialic acid to the 6-position of a galactose residue in a sugar chain of a glycoconjugate or the 6-position of a galactose residue in a free sugar chain, or to the 6-position of a monosaccharide having a hydroxyl group on carbon at the 6-position and being capable of forming an oligosaccharide or a glycoconjugate.
摘要:
This invention provides to a novel gene encoding a protein aving the activity of &bgr;-galactoside-&agr;2,6-sialyltransferase. The gene of the invention encodes a protein which having the amino acid sequence of SEQ ID NO:1, or a protein having the amino acid sequence of SEQ ID NO:1 which have been modified by deletion, substitution or addition of at least one amino acid residue in said sequence, while maintaining substantially the same &bgr;-galactoside-&agr;2,6-sialyltransferase activity. The present invention uses said gene to further provide a vector for expressing a protein having the &bgr;-galactoside-&agr;2,6-sialyltransferase activity, host cells and a recombinant protein. The protein encoded by the gene of the invention does not have any substantial homology with known sialyltransferases, and in addition, the membrane binding region is located in the C-terminal unlike known sialyltransferases.
摘要翻译:本发明提供了一种新型的编码β-半乳糖苷-α2,6-唾液酸转移酶活性蛋白质的基因。本发明的基因编码具有SEQ ID NO:1的氨基酸序列的蛋白质,或具有 通过在所述序列中缺失,取代或添加至少一个氨基酸残基而修饰的SEQ ID NO:1的氨基酸序列,同时保持基本上相同的β-半乳糖苷-α2,6-唾液酸转移酶活性。本发明使用 所述基因进一步提供用于表达具有β-半乳糖苷-α2,6-唾液酸转移酶活性的蛋白质的载体,宿主细胞和重组蛋白。 由本发明的基因编码的蛋白质与已知的唾液酸转移酶不具有任何实质的同源性,此外,与已知的唾液酸转移酶不同,膜结合区位于C末端。
摘要:
The present inventions relate to novel .alpha.-agarase which selectively hydrolyzes the 1,3-bond of an oligosaccharide derived from agarose; a process for producing oligosaccharides and/or monosaccharides, characterized by that a .beta.-agarase which acts on both polysaccharides and oligosaccharides derived from agarose and an .alpha.-agarase which acts specifically on oligosaccharides derived from agarose are allowed to react with a substrate which contains agarose or an oligosaccharide derived from agarose; and a process for producing oligosaccharides and/or monosaccharides, characterized by that the above .alpha.-agarase is allowed to react with a substrate which contains an oligosaccharide derived from agarose.
摘要:
To obtain a nonwoven fabric which is excellent in the heat resistance and the chemical resistance, of which the fiber diameter is small, and which is excellent in the mechanical strength at a temperature at which it is used; and an electrolyte membrane which is excellent in the dimensional stability when it is swollen by water, and of which an increase in the resistance by a reinforcing material is suppressed.A nonwoven fabric 28 containing fibers 26 of an ethylene/tetrafluoroethylene copolymer having a storage elastic modulus E′ at 25° C. of at least 8×108 Pa and a melt viscosity measured at 300° C. of higher than 60 Pa·s and at most 300 Pa·s, wherein the average fiber diameter of the fibers is from 0.01 to 3 μm; and an electrolyte membrane reinforced by the nonwoven fabric 28.
摘要:
A process cartridge detachably mountable to a main assembly of an electrophotographic image forming apparatus which includes an openable closing member, a first main assembly guide movable in interrelation with opening and closing action of the closing member, and a second main assembly guide, the process cartridge include a photosensitive drum; a first cartridge frame portion extending in a cartridge mounting direction to the main assembly, at one axial end portion of the drum; a first cartridge guide projected from the first cartridge frame portion, the first cartridge guide moving the cartridge toward a cartridge mounting position by movement of the first main assembly guide with the cartridge being supported on first main assembly guide, upon mounting of the cartridge to the main assembly; a second cartridge frame portion extended in the mounting direction at the other axial end portion of the drum; a second cartridge guide projected from the second cartridge frame portion, the second cartridge guide moving the cartridge toward a cartridge mounting position by movement of the second main assembly guide with the cartridge being supported on the second main assembly guide; when the cartridge is mounted to the main assembly of the apparatus; a first cartridge positioning portion for engaging with a first main assembly positioning portion provided in the main assembly to position the cartridge relative to the main assembly, the first cartridge positioning portion being projected outwardly from the first cartridge frame portion and coaxially with the drum at the one axial end of the drum; and a second cartridge positioning portion for engaging with a second main assembly positioning portion to position the cartridge relative to the main assembly when the cartridge is mounted to the main assembly, the second cartridge positioning portion being projected outwardly from the second cartridge frame portion and coaxially with the drum at the other axial end of the drum.
摘要:
An electrophotographic image forming apparatus onto which a process cartridge is detachably mountable, for forming an image on a recording medium, has (a) a mounting member for detachably mounting a process cartridge having an electrophotographic photosensitive member, a charging member for charging the electrophotographic photosensitive member, a cartridge charge bias contact for receiving a charging bias to be impressed on the charging member from the main body of the apparatus when the process cartridge is mounted on the main body of the apparatus, and a cartridge-earth contact for connecting the electrophotographic photosensitive member to ground with the main body of the apparatus when the process cartridge is mounted on the main body of the apparatus, (b) a main-body-charge bias contact to be electrically connected to the cartridge-charge-bias contact when the process cartridge is mounted on the mounting member, (c) a main-body earth contact to be electrically connected to the cartridge-earth contact when the process cartridge is mounted on the mounting member, (d) a detecting member for detecting that the process cartridge is not mounted in a cartridge-mounting position by detecting that at least one of the cartridge-charge-bias contact and the cartridge-earth contact is not electrically connected to a corresponding one of the main-body-charge bias contact and the main-body earth contact and (e) a conveying member for conveying the recording medium.
摘要:
An apparatus for producing deionized water consisting essentially of an electrodialyzer having cation exchange membranes and anion exchange membranes alternately arranged between a cathode and an anode to form demineralizing compartments and concentrating compartments, and ion exchangers accommodated in the demineralizing compartments, wherein a pressure of from 0.1 to 20 kg/cm.sup.2 is exerted between the ion exchangers accommodated in the demineralizing compartments and the cation exchange membranes and anion exchange membranes defining the demineralizing compartments.
摘要:
A porous ion exchanger which has a porous structure having ion exchange resin particles bound by a binder polymer, and which has a water permeability of at least 30 kg.multidot.cm.sup.-1 .multidot.hr.sup.-1 under a pressure of 0.35 kg/cm.sup.2.
摘要:
An anion exchanger consisting essentially of an aromatic polysulfone block copolymer of the formula: ##STR1## wherein Ar is ##STR2## or ##STR3## X is --SO.sub.2 --, ##STR4## --S-- or --O--, Y is --SO.sub.2 --, --S-- or --O--, Z is a single bond, --O--, --S--, --SO.sub.2 --, ##STR5## each of R.sub.1 to R.sub.9 which may be the same or different is a hydrocarbon group having from 1 to 8 carbon atoms, each of a to d is an integer of from 0 to 4, e is an integer of from 0 to 3, (f+g) is from 0 to 7, (h+i) is from 0 to 5, each of R.sub.10 and R.sub.11 is a hydrogen atom or a hydrocarbon group having from 1 to 6 carbon atoms, and each of m and n is an integer of from 2 to 200, provided m/n=100/1-1/10, and having anion exchange groups introduced to the aromatic rings of the copolymer.