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    Production of vitamin B.sub.6 by fermentation
    4.
    发明授权
    Production of vitamin B.sub.6 by fermentation 失效
    通过发酵生产维生素B6

    公开(公告)号：US5766894A

    公开(公告)日：1998-06-16

    申请号：US709068

    申请日：1996-09-06

    申请人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

    发明人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

    IPC分类号： C12P17/12 ,  C12R1/41

    CPC分类号： C12P17/12

    摘要： A process for producting vitamin B.sub.6 comprises cultivating a microorganism belonging to the genus Rhizobium and being capable of producing vitamin B.sub.6 in a culture medium under aerobic conditions, and separating the resulting vitamin B.sub.6 from the fermentation broth. As well as containing assimilable carbon sources, digestible nitrogen sources, inorganic salts and other nutrients necessary for the growth of the microorganism, the culture medium preferably additionally contains pyruvate, D-glyceraldehyde, glycolaldehyde, glycine, 1-deoxy-D-threo-pentulose, 4-hydroxy-L-threonine or a mixture thereof. This process affords high yields of vitamin B.sub.6, a vitamin essential for the nutrition of animals, plants and microorganisms and useful as a medicine and in foodstuffs.

    摘要翻译： 生产维生素B6的方法包括培养属于根瘤菌属的微生物并且在需氧条件下能够在培养基中产生维生素B6，并将所得到的维生素B6与发酵液分离。 除了含有可吸收的碳源，可消化的氮源，无机盐和微生物生长所必需的其他营养物之外，培养基优选另外含有丙酮酸，D-甘油醛，乙二醇醛，甘氨酸，1-脱氧-D-苏糖 - ，4-羟基-L-苏氨酸或其混合物。 该方法提供高产量的维生素B6，维生素B6是维持动物，植物和微生物营养所必需的维生素，可用作药物和食品。

    Process of producing antibiotic SM-173B with Streptomyces chromofuscus
    6.
    发明授权
    Process of producing antibiotic SM-173B with Streptomyces chromofuscus 失效
    生产抗生素SM-173B与链霉菌（Streptomyces chromofuscus）的方法

    公开(公告)号：US4304861A

    公开(公告)日：1981-12-08

    申请号：US92402

    申请日：1979-11-08

    申请人： Akiko Fujiwara ,  Mitsuhiko Fujiwara ,  Tatsuo Hoshino ,  Yuzuru Sekine ,  Masaaki Tazoe

    发明人： Akiko Fujiwara ,  Mitsuhiko Fujiwara ,  Tatsuo Hoshino ,  Yuzuru Sekine ,  Masaaki Tazoe

    IPC分类号： C12P29/00 ,  A61K31/65 ,  A61P31/04 ,  C07G11/00 ,  C12P15/00

    CPC分类号： C07G11/00 ,  Y10S435/886

    摘要： A novel antibiotic having the ascribed formula ##STR1## is provided. They physical characteristics are as follows: (a) Elementary analysis: Calculated (for C.sub.20 H.sub.16 O.sub.7): C,65.22; H,4.35%. Found: C,64.60; H,4.34%.(b) Molecular weight (mass-spectrometric): 368.(c) Melting point: 225.degree.-226.degree. C.(d) Specific rotation: [.alpha.].sub.D .sup.20 =+123.5.degree. (c=0.2 in chloroform).(e) Ultraviolet absorption spectrum:.lambda..sub.max .sup.methanol =235 nm (.epsilon.=28600); 262 nm (.epsilon.=21900); 434-435 nm (.epsilon.=11300);.lambda..sub.max .sup.0.1-N NaOH/methanol =246 nm (.epsilon.=22400); 256 nm (.epsilon.=23200); 523-524 nm (.epsilon.=9600).(f) Infrared absorption spectrum: 3500, 1710, 1672, 1628, 1600, 1575, 1472, 1452, 1420, 1390, 1295, 1255, 1218, 1200, 1182, 1105, 1025, 1005, 962, 938, 848, 765, 752, 730 cm.sup.-1.(g) Solubility: Soluble in methanol, ethanol, butanol, acetone, chloroform, ethyl acetate and in water under alkaline conditions; poorly soluble in n-hexane; insoluble in petroleum ether and water.(h) Color reactions: Positive with sulfuric acid and iodine; negative with ninhydrin and anisaldehyde.(i) NMR spectrum in DC.Cl.sub.3 : 1.42 ppm (3H, singlet), 3.02 ppm (1H, quartet), 3.43 ppm (3H, singlet), 3.62 ppm (1H, triplet), 3.64 ppm (1H, singlet), 3.93 ppm (1H, triplet), 7.31-3.41 ppm (4H, olefinic protons), 12.0 ppm (1H, singlet), 12.5 ppm (1H, singlet).Also provided is a process for the antibiotic's production.

    摘要翻译： 提供具有归属公式的新型抗生素。 它们的物理特性如下：（a）元素分析：计算值（C 20 H 16 O 7）：C，65.22; H，4.35％。 实测值：C，64.60; H，4.34％。 （b）分子量（质谱）：368.（c）熔点：225°-226℃。（d）比旋光度：αD20 = + 123.5°（氯仿中c = 0.2）。 （e）紫外吸收光谱：λmax甲醇= 235nm（ε= 28600）; 262nm（ε= 21900）; 434-435nm（ε= 11300）; λmax 0.1-N NaOH /甲醇= 246nm（ε= 22400）; 256nm（ε= 23200）; 523-524nm（ε= 9600）。 （f）红外吸收光谱：3500,1710,1672,1628,1600,1575,1472,1452,1420,1390,1295,1255,1218,1200,1122,1105,1025,1005，962，938,848,765 ，752,730cm-1。 （g）溶解性：在碱性条件下，溶于甲醇，乙醇，丁醇，丙酮，氯仿，乙酸乙酯和水; 难溶于正己烷; 不溶于石油醚和水。 （h）显色反应：用硫酸和碘阳性; 用茚三酮和茴香醛阴性。 （i）DC中的NMR光谱：3.43ppm（3H，单峰），3.02ppm（1H，四重峰），3.43ppm（3H，单峰），3.62ppm（1H，三重峰） 3.93ppm（1H，三重峰），7.31-3.41ppm（4H，烯属质子），12.0ppm（1H，单峰），12.5ppm（1H，单峰）。 还提供了抗生素生产的过程。

    Gene encoding vitamin b6 phosphate phosphatase and use thereof
    7.
    发明授权
    Gene encoding vitamin b6 phosphate phosphatase and use thereof 有权
    编码维生素B6磷酸磷酸酶的基因及其用途

    公开(公告)号：US07598066B2

    公开(公告)日：2009-10-06

    申请号：US10528845

    申请日：2003-09-23

    申请人： Tatsuo Hoshino ,  Yoshie Nagahashi ,  Masaaki Tazoe

    发明人： Tatsuo Hoshino ,  Yoshie Nagahashi ,  Masaaki Tazoe

    IPC分类号： C12N9/16 ,  C12N15/70 ,  C12N1/21 ,  C12N1/16 ,  C12P21/06 ,  C12P17/00 ,  C12P17/12 ,  C07H21/04

    CPC分类号： C12N9/16 ,  C12P17/12

    摘要： Disclosed is an isolated DNA encoding vitamin B6 phosphate phosphatase selected from the group consisting of: (a) a DNA sequence represented in SEQ ID NO:9; (b) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and hybridizes under standard conditions to the DNA sequence defined in (a) or a fragment of thereof; (c) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity, wherein said polypeptide is at least 70% identical to the amino acid sequence represented in SEQ ID NO:10; (d) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and is at least 70% identical to the DNA sequence represented in SEQ ID NO:9; (e) a degenerate DNA sequence of any one of (a) to (c).

    摘要翻译： 公开了编码选自以下的维生素B6磷酸磷酸酶的分离DNA：（a）SEQ ID NO：9所示的DNA序列; （b）编码具有维生素B6磷酸酯酶活性的多肽的DNA序列，并在标准条件下与（a）中定义的DNA序列或其片段杂交; （c）编码具有维生素B6磷酸酯酶活性的多肽的DNA序列，其中所述多肽与SEQ ID NO：10所示氨基酸序列至少70％相同; （d）编码具有维生素B6磷酸酯酶活性并与SEQ ID NO：9所示DNA序列至少70％相同的多肽的DNA序列; （e）（a）至（c）中任一项的简并DNA序列。

    Gene encoding vitamin b6 phosphate phosphatase and use thereof
    8.
    发明申请
    Gene encoding vitamin b6 phosphate phosphatase and use thereof 有权
    编码维生素B6磷酸磷酸酶的基因及其用途

    公开(公告)号：US20060263862A1

    公开(公告)日：2006-11-23

    申请号：US10528845

    申请日：2003-09-23

    申请人： Tatsuo Hoshino ,  Yoshie Nagahashi ,  Masaaki Tazoe

    发明人： Tatsuo Hoshino ,  Yoshie Nagahashi ,  Masaaki Tazoe

    IPC分类号： C12P17/00 ,  C12N9/16 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/16 ,  C12P17/12

    摘要： Disclosed is an isolated DNA encoding vitamin B6 phosphate phosphatase selected from the group consisting of: (a) a DNA sequence represented in SEQ ID NO:9; (b) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and hybridizes under standard conditions to the DNA sequence defined in (a) or a fragment of thereof; (c) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity, wherein said polypeptide is at least 70% identical to the amino acid sequence represented in SEQ ID NO: 10; (d) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and is at least 70% identical to the DNA sequence represented in SEQ ID NO:9; (e) a degenerate DNA sequence of any one of (a) to (c).

    摘要翻译： 公开了编码选自以下的维生素B 6磷酸磷酸酶的分离DNA：（a）SEQ ID NO：9所示的DNA序列; （b）编码具有维生素B 6磷酸磷酸酶活性的多肽的DNA序列，并在标准条件下与（a）中定义的DNA序列或其片段杂交; （c）编码具有维生素B 6磷酸磷酸酶活性的多肽的DNA序列，其中所述多肽与SEQ ID NO：10所示氨基酸序列至少70％相同; （d）编码具有维生素B 6磷酸磷酸酶活性的多肽的DNA序列，与SEQ ID NO：9所示的DNA序列至少70％相同; （e）（a）至（c）中任一项的简并DNA序列。

    Genetically engineered L-sorbose reductase-deficient mutants
    9.
    发明授权
    Genetically engineered L-sorbose reductase-deficient mutants 有权
    遗传工程L-山梨糖还原酶缺陷突变体

    公开(公告)号：US06664082B1

    公开(公告)日：2003-12-16

    申请号：US09266320

    申请日：1999-03-11

    申请人： Tatsuo Hoshino ,  Takahide Kon ,  Masako Shinjoh ,  Masaaki Tazoe

    发明人： Tatsuo Hoshino ,  Takahide Kon ,  Masako Shinjoh ,  Masaaki Tazoe

    IPC分类号： C12P1902

    CPC分类号： C12N9/0004 ,  C12N15/102

    摘要： The present invention provides a genetically engineered microorganism belonging to the genus Gluconobacter or Acetobacter, which has an engineered gene for the biological activity of reducing L-sorbose which is more than 90% non-functional in developing the said biological activity. The engineered microorganism is derived from a microorganism belonging to the genus Gluconobacter or Acetobacter, and has the biological activity for reducing L-sorbose that is less than 10% of the amount of the activity of the wild type organism. The present invention further provides a method for producing the genetically engineered microorganism by mutating the gene of a microorganism of the genus Gluconobacter or Acetobacter which encodes a protein having the L-sorbose reductase activity, determining the L-sorbose reductase activity of the resulting microorganism with the mutated gene and selecting the genetically engineered microorganism having L-sorbose reductase activity that is less than 10% of the amount of the activity of the wild type microorganism.

    摘要翻译： 本发明提供属于葡萄糖酸杆菌属或醋杆菌属的遗传工程微生物，其具有用于降低L-山梨糖的生物活性的工程化基因，其在发展所述生物活性时超过90％不起作用。 工程微生物来自属于葡糖杆菌属或醋杆菌属的微生物，具有小于野生型生物活性量的10％的L-山梨糖的生物活性。 本发明还提供了通过使编码具有L-山梨糖还原酶活性的蛋白质的葡糖酸杆菌属或醋酸杆菌属的微生物的基因突变来生产遗传工程微生物的方法，用所得的微生物测定所得微生物的L-山梨糖还原酶活性 突变基因并选择具有小于野生型微生物活性量的10％的L-山梨糖还原酶活性的遗传工程微生物。

    Production of vitamin B.sub.6 with an enzyme-containing cell extract
    10.
    发明授权
    Production of vitamin B.sub.6 with an enzyme-containing cell extract 失效
    用含酶细胞提取物生产维生素B6

    公开(公告)号：US6060267A

    公开(公告)日：2000-05-09

    申请号：US291718

    申请日：1999-04-14

    申请人： Tatsuo Hoshino ,  Masaaki Tazoe

    发明人： Tatsuo Hoshino ,  Masaaki Tazoe

    IPC分类号： C12P17/12 ,  A23L1/30 ,  A61K51/00 ,  C12N1/00 ,  C12P1/00 ,  C12P1/04

    CPC分类号： C12P17/12 ,  Y10S435/822

    摘要： A process for the enzymatic production of vitamin B.sub.6 which includes incubating 1-deoxy-D-threo-pentulose and 4-hydroxy-L-threonine with an enzyme system that is cell free or essentially cell free and prepared from the cells of a microorganism belonging to the genus Rhizobium, Sinorhizobium, Flavobacterium, Chryseobacterium, Lactobacillus, Arthrobacter, Bacillus, Klebsiella, Escherichia, Pseudomonas, Stenotrophomonas, Enterobacter, Serratia, Corynebacterium, Brevibacterium, Exiguobacterium, Saccharomyces, Yamadazyma, Pichia or Candida, in the presence of NADP.sup.+, NAD.sup.+, ATP. Manganese and magnesium ions stimulate the above reaction. This process affords high yields of vitamin B.sub.6, a vitamin essential for the nutrition of animals, plants and microorganisms, and which is also useful as a medicine or food additive. In addition, an enzyme reaction system for producing vitamin B.sub.6 and a process for making the enzyme reaction system are also provided.

    摘要翻译： 用于酶促生产维生素B6的方法，其包括用无细胞或基本上不含细胞的酶系统温育1-脱氧-D-苏糖 - 戊酮糖和4-羟基-L-苏氨酸并由所属微生物的细胞制备 在NADP +，NAD +的存在下，根瘤菌属，根瘤菌属，中华根瘤菌属，黄杆菌属，巧克力杆菌属，乳杆菌属，节杆菌属，芽孢杆菌属，克雷伯菌属，埃希氏杆菌属，假单胞菌属，嗜麦芽寡养单胞菌属，肠杆菌属，沙雷氏菌属，棒状杆菌属，短杆菌属，精氨酸杆菌属，酵母属，Yamadazyma，毕赤酵母或假丝酵母属， ，ATP。 锰和镁离子刺激上述反应。 该方法提供高产量的维生素B6，维生素B6是动物，植物和微生物营养必需的维生素，也可用作药物或食品添加剂。 此外，还提供了用于生产维生素B6的酶反应体系和制备酶反应体系的方法。