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公开(公告)号：US20240191223A1
公开(公告)日：2024-06-13
申请号：US18334074
申请日：2023-06-13
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
CPC分类号： C12N15/102 , C12N9/22 , C12N15/111 , C12N15/113 , C12N15/8213 , C12N15/8509 , C12N2310/20 , C12N2800/22
摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
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公开(公告)号：US20240182928A1
公开(公告)日：2024-06-06
申请号：US18235760
申请日：2023-08-18
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College , Rutgers, The State University of New Jersey , Skolkovo Institute of Science and Technology , The United States of America, as represented by the Secretary, Dept. of Health and Human Services
发明人： Konstantin Severinov , Feng Zhang , Yuri I. Wolf , Sergey Shmakov , Ekaterina Semenova , Leonid Minakhin , Kira S. Makarova , Eugene Koonin , Silvana Konermann , Julia Joung , Jonathan S. Gootenberg , Omar O. Abudayyeh , Eric S. Lander
IPC分类号： C12N15/90 , C12N9/22 , C12N15/10 , C12N15/11 , C12N15/113 , C12N15/63 , C12N15/82 , C12N15/85
CPC分类号： C12N15/907 , C12N9/22 , C12N15/102 , C12N15/111 , C12N15/113 , C12N15/63 , C12N15/8201 , C12N15/85 , C12N2310/111 , C12N2310/20 , C12N2800/22
摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
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公开(公告)号：US20240132916A1
公开(公告)日：2024-04-25
申请号：US18276471
申请日：2022-02-09
发明人： Feng Zhang , Michael Segel , Alim Ladha , Michelle Walsh , Christopher Frangieh
CPC分类号： C12N15/907 , C12N9/22 , C12N15/11 , C12N2310/20 , C12N2800/90
摘要： Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Novel nucleic acid targeting systems comprise components of CRISPR systems and non-LTR retrotransposon elements.
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公开(公告)号：US20240110203A1
公开(公告)日：2024-04-04
申请号：US18270854
申请日：2022-01-07
发明人： Feng Zhang , Jonathan Strecker , Guilhem Faure
CPC分类号： C12N15/907 , C07K14/47 , C12N9/22 , C12N15/11 , C12N2310/20 , C12N2800/90
摘要： The present application provides systems, methods and compositions used for targeted gene modification, targeted insertion, perturbation of gene transcripts, nucleic acid editing. Novel nucleic acid targeting systems comprise components of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems and transposable elements. Specifically, the disclosure provides an engineered composition comprising: a programmable DNA-binding protein and two or more Tn7-like transposition proteins, wherein at least one of the Tn7-like transposition proteins is connected to the DNA-binding protein or otherwise capable of forming a complex with the DNA-binding protein, wherein the DNA-binding protein comprising a Cas protein including a Cas12k protein, and wherein two or more Tn7-like transposition proteins consisting of TnsB, TnsC, and TniQ.
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公开(公告)号：US20230340505A1
公开(公告)日：2023-10-26
申请号：US18128122
申请日：2023-03-29
发明人： Feng Zhang
IPC分类号： C12N15/74 , C12N15/113 , C12N9/22 , C12N15/63 , G16B20/30 , G16B30/10 , G16B20/20 , G16B20/50 , G16B20/00 , C12N15/85 , C12N15/70 , C12N15/90
CPC分类号： C12N15/746 , C12N15/113 , C12N9/22 , C12N15/63 , G16B20/30 , G16B30/10 , G16B20/20 , G16B20/50 , G16B20/00 , C12N15/85 , C12N15/70 , C12N15/74 , C12N15/8509 , C12N15/907 , C12N2310/3519 , C12N2310/531 , C12N2310/20 , C12N15/102
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
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公开(公告)号：US20230235285A1
公开(公告)日：2023-07-27
申请号：US18148886
申请日：2022-12-30
申请人： The Children's Medical Center Corporation , The Broad Institute, Inc. , Massachusetts Institute of Technology
发明人： Daniel E. Bauer , Stuat H. Orkin , Neville Sanjana , Ophir Shalem , Feng Zhang
CPC分类号： C12N5/0641 , C12N15/102 , C12N9/22 , C12N15/11 , C12N15/86 , C12N2510/00 , C12N2310/20 , C12N2740/15041
摘要： Provided herein are nucleic acid molecules that target the BCL11A enhancer functional regions, compositions comprising the nucleic acid molecules and methods for increasing fetal hemoglobin levels in a cell by disrupting BCL11A expression at the genomic level. Also provided herein are methods and compositions relating to the treatment of hemoglobinopathies by reinduction of fetal hemoglobin levels. In particular, the nucleic acid molecules target the +62, +58, and/or the +55 enhancer functional regions.
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公开(公告)号：US11597919B2
公开(公告)日：2023-03-07
申请号：US16592744
申请日：2019-10-03
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , University of Tokyo , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Silvana Konermann , Alexandro Trevino , Mark Brigham , Fei Ran , Patrick Hsu , Chie-yu Lin , Osamu Nureki , Hiroshi Nishimasu , Ryuichiro Ishitani , Feng Zhang
IPC分类号： C12N9/22 , A61K38/46 , A61K48/00 , C12N15/113 , C12N15/90 , C12Q1/6876 , A61P43/00
摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.
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公开(公告)号：US20230049737A1
公开(公告)日：2023-02-16
申请号：US17786168
申请日：2020-12-30
发明人： Feng Zhang , Jonathan Strecker , David Li
摘要： Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Novel nucleic acid targeting systems comprise components of CRISPR systems, reverse transcriptase, pegRNAs, paired pegRNAs or modified pegRNAs, DNA processing proteins, recombinases, proteins for inhibiting nucleases, and proteins for promoting ssDNA annealing.
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公开(公告)号：US20220380758A1
公开(公告)日：2022-12-01
申请号：US17773104
申请日：2020-10-30
发明人： Makoto Saito , Feng Zhang , Guilhem Faure
摘要： Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Novel nucleic acid targeting systems comprise components of CRISPR systems and transposable elements.
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公开(公告)号：US20220333208A1
公开(公告)日：2022-10-20
申请号：US17640016
申请日：2020-09-03
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , DANA-FARBER CANCER INSTITUTE, INC.
发明人： Jonathan Gootenberg , Omar Abudayyeh , Jeremy Koob , Rahul Vedula , Coleman Lindsley , Feng Zhang
IPC分类号： C12Q1/6886 , C12Q1/682
摘要： Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences, including multiplex lateral flow diagnostic devices and methods of use, including for detection of cancer markers, are provided.
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