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1.发明申请公开(公告)号：US20210340477A1
公开(公告)日：2021-11-04
申请号：US17246028
申请日：2021-04-30
发明人： Jonathan R. Coppeta , Hesham Azizgolshani , Brian P. Cain , Brett C. Isenberg , Joseph L. Charest , Else M. Vedula , Ashley L. Gard , Ryan S. Maloney , Jeffrey T. Borenstein , Rebeccah Lulu
摘要： The present disclosure describes systems and methods for providing culturing of a number of various tissue types in an air-liquid configuration in a high-throughput format and allowing co-culture of cells as well as application of physiologically relevant flow. A microfluidic cell culturing device is provided that includes a first channel having a first inlet port and a second inlet port, the first channel defined in a first layer. The microfluidic cell culturing device includes a membrane layer having a first surface coupled to the first layer defining the first channel, the membrane layer comprising semipermeable membrane that forms at least a portion of a surface of the first channel. The microfluidic cell culturing device includes a chamber defined in a second layer that exposes a portion the membrane layer to an external environment, wherein the chamber overlaps a portion of the first channel across the membrane layer.
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公开(公告)号：US20190310260A1
公开(公告)日：2019-10-10
申请号：US16430787
申请日：2019-06-04
IPC分类号： G01N33/574 , G01N33/58 , C12Q1/6834 , G01N33/543 , C12Q1/68
摘要： A multiplex-able, regeneratable nucleic-acid linked immunoassay method and system for the detection of a single specific, or multiple, soluble analytes in solution and regeneratable biosensor devices for same are described.
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公开(公告)号：US11701652B2
公开(公告)日：2023-07-18
申请号：US17031146
申请日：2020-09-24
发明人： Hesham Azizgolshani
IPC分类号： C12N15/10 , B01L3/00 , B01L7/00 , F28F3/12 , G01N35/10 , B29C45/16 , B29K25/00 , B29K105/00 , B29L31/00
CPC分类号： B01L3/502707 , B29C45/1671 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2300/042 , B01L2300/123 , B01L2300/168 , B29C2045/1673 , B29K2025/06 , B29K2105/0085 , B29L2031/756
摘要： A method for manufacturing a microfluidic device can include providing a base component to define a first portion of the microfluidic device. A cap component of the microfluidic device can be fabricated with a sealing lip extending a first distance from a first side of the cap component and a support portion extending a second distance, less than the first distance, from the first side of the cap component. The method can include positioning the cap component and the base component within a mold to bring the sealing lip of the cap component in contact with the base component. The base component, the support portion of the cap component, and the sealing lip of the cap component together can define a cavity. The method can include injecting a polymer material into the mold to cause the polymer material to fill the cavity.
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公开(公告)号：US20210162416A1
公开(公告)日：2021-06-03
申请号：US17108475
申请日：2020-12-01
发明人： Jeffrey Borenstein , Nathan Moore , Daniel Doty , Timothy Haggerty , Joseph Charest , Alla Gimbel , Vienna Mott , Brett Isenberg , Hesham Azizgolshani , Brian Cain , Mark Mescher
摘要： A microfluidic device for modeling a tumor-immune microenvironment can include a multiwell plate defining a plurality of microenvironment units fluidically coupled with a plurality of wells. Each microenvironment unit of the plurality of microenvironment units can include one or more compartments. Each microenvironment unit can include a trapping feature positioned within the one or more compartments. The trapping feature can be defined by a portion of at least one of a sidewall or a floor of the one or more compartments. The trapping feature can restrict movement of a tissue sample introduced into the one or more compartments and to allow fluid to flow past the tissue sample. The microfluidic device can include a plurality of micropumps each coupled with a respective well and configured to control movement of a respective fluid sample through each respective well.
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公开(公告)号：US20210086178A1
公开(公告)日：2021-03-25
申请号：US17031146
申请日：2020-09-24
发明人： Hesham Azizgolshani
摘要： A method for manufacturing a microfluidic device can include providing a base component to define a first portion of the microfluidic device. A cap component of the microfluidic device can be fabricated with a sealing lip extending a first distance from a first side of the cap component and a support portion extending a second distance, less than the first distance, from the first side of the cap component. The method can include positioning the cap component and the base component within a mold to bring the sealing lip of the cap component in contact with the base component. The base component, the support portion of the cap component, and the sealing lip of the cap component together can define a cavity. The method can include injecting a polymer material into the mold to cause the polymer material to fill the cavity.
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公开(公告)号：US20230073620A1
公开(公告)日：2023-03-09
申请号：US17821144
申请日：2022-08-19
摘要： Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.
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公开(公告)号：US20220259536A1
公开(公告)日：2022-08-18
申请号：US17650765
申请日：2022-02-11
发明人： Samuel Kann , Joseph L. Charest , Else M. Vedula , Erin M. Shaughnessey , Hesham Azizgolshani , Brett Isenberg , Jonathan R. Coppeta
摘要： A microchannel cell culture device is disclosed. The microchannel cell culture device includes a well plate defining an array of tissue modeling environments. A cell culture system including the microchannel cell culture device is also disclosed. The cell culture system includes a plurality of optical sensors, a platform, and a light source. A method of high throughput screening cell biological activity with the microchannel cell culture device is disclosed. A method of measuring oxygen consumption rate of cells in the microchannel cell culture device is disclosed. A method of facilitating drug development with the microchannel cell culture device is also disclosed.
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公开(公告)号：US20180149656A1
公开(公告)日：2018-05-31
申请号：US15826494
申请日：2017-11-29
IPC分类号： G01N33/574 , C12Q1/68 , G01N33/58
CPC分类号： G01N33/57488 , C07K2317/32 , C12Q1/68 , C12Q1/6834 , C12Q2521/543 , C12Q2525/173 , C12Q2565/518 , G01N33/54353 , G01N33/58 , C12Q2565/607
摘要： A multiplex-able, regeneratable nucleic-acid linked immunoassay method and system for the detection of a single specific, or multiple, soluble analytes in solution and regeneratable biosensor devices for same are described.
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