公开(公告)号：US20240318225A1

公开(公告)日：2024-09-26

申请号：US18293305

申请日：2022-07-29

Applicant: General Electric Company ,  The Penn State Research Foundation

Inventor： Christopher Michael Puleo ,  Erik Leeming Kvam ,  Pak Kin Wong ,  Peter Torab ,  Christine Lynne Surrette

IPC: C12Q1/24 ,  C12Q1/18 ,  G01N33/49

CPC classification number: C12Q1/24 ,  C12Q1/18 ,  G01N33/491

Abstract: The subject matter of the present disclosure generally relates to techniques for isolating bacterial cells from a biological sample comprising red blood cells. Using an aggregating agent and an anticoagulant during sedimentation permits separation of bacterial pathogens in the sample from red blood cells. The separated sedimentation layer, which is enriched in any bacterial pathogens, can be centrifuged and resuspended to concentrate the bacteria for additional analysis, such as bacterial identification and/or antibiotic susceptibility tests.

公开(公告)号：US12233419B2

公开(公告)日：2025-02-25

申请号：US17259136

申请日：2019-07-11

Applicant: The Penn State Research Foundation

Inventor： Pak Kin Wong ,  Hui Li

IPC: B01L3/00 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/689

Abstract: Provided are devices and methods for detecting the presence, absence, and/or characteristics of bacteria. A microfluidic device for characterizing bacteria is provided. The device contains one or more bacteria trapping channels, and one or more pneumatic channels in contact with the bacteria trapping channels, wherein the one or more pneumatic channels are configured to reduce the height of the one or more bacteria trapping channels to thereby trap bacteria in the one or more bacteria trapping channels if said bacteria are present in a liquid biological sample that is introduced into the microfluidic device. Also provided is a method for identifying bacteria that may be used with the microfluidic device.

公开(公告)号：US11938476B2

公开(公告)日：2024-03-26

申请号：US17749720

申请日：2022-05-20

Applicant: The Penn State Research Foundation

Inventor： Pak Kin Wong ,  Tak-Sing Wong ,  Jing Wang ,  Hui Li ,  Yi Lu ,  Ying Wan

IPC: B01L3/00

CPC classification number: B01L3/5025 ,  B01L3/50273 ,  B01L2200/00 ,  B01L2300/0816 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0481

Abstract: A biochemical analysis system capable of sample preparation and processing can include at least one inlet channel having a non-fouling, slippery surface to autonomously transport a fluid sample to a chamber by a geometry of the at least one inlet channel. The at least one inlet channel can include a first end, which is open and exposed, and a second end connected to the chamber for mixing and reaction of the fluid sample, and the at least one inlet channel can include a converging or diverging angle.

公开(公告)号：US11351536B2

公开(公告)日：2022-06-07

申请号：US16174008

申请日：2018-10-29

Applicant: The Penn State Research Foundation

Inventor： Pak Kin Wong ,  Tak-Sing Wong ,  Jing Wang ,  Hui Li ,  Yi Lu ,  Ying Wan

IPC: B01L3/00

Abstract: A biochemical analysis system capable of sample preparation and processing can include at least one inlet channel having a non-fouling, slippery surface to autonomously transport a fluid sample to a chamber by a geometry of the at least one inlet channel. The at least one inlet channel can include a first end, which is open and exposed, and a second end connected to the chamber for mixing and reaction of the fluid sample, and the at least one inlet channel can include a converging or diverging angle.