公开(公告)号：US20190276842A1

公开(公告)日：2019-09-12

申请号：US16422824

申请日：2019-05-24

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C07K19/00 ,  C12N15/113 ,  C12N9/22 ,  C12N15/62 ,  C12N15/52 ,  C12N9/10 ,  C12N15/74 ,  C12N15/88

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US20240167052A1

公开(公告)日：2024-05-23

申请号：US18513347

申请日：2023-11-17

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian F. Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C07K19/00 ,  C12N9/10 ,  C12N9/22 ,  C12N15/10 ,  C12N15/113 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/86 ,  C12N15/88 ,  C12N15/90

CPC classification number: C12N15/8509 ,  C07K19/00 ,  C12N9/1007 ,  C12N9/1025 ,  C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/85 ,  C12N15/86 ,  C12N15/88 ,  C12N15/902 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/06 ,  C07K2319/09 ,  C07K2319/20 ,  C12N2310/20 ,  C12N2740/13043 ,  C12N2740/16043 ,  C12N2750/14143

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US20210284981A1

公开(公告)日：2021-09-16

申请号：US17258047

申请日：2019-07-23

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian F. Banfield ,  David Burstein ,  Steven C. Strutt ,  Lucas Benjamin Harrington

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  A61K48/00

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RKAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US11795472B2

公开(公告)日：2023-10-24

申请号：US16422824

申请日：2019-05-24

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/113 ,  C12N15/86 ,  C07K19/00 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/88 ,  C12N15/10 ,  C12N15/90 ,  C12N9/10

CPC classification number: C12N15/8509 ,  C07K19/00 ,  C12N9/1007 ,  C12N9/1025 ,  C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/85 ,  C12N15/86 ,  C12N15/88 ,  C12N15/902 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/06 ,  C07K2319/09 ,  C07K2319/20 ,  C12N2310/20 ,  C12N2740/13043 ,  C12N2740/16043 ,  C12N2750/14143

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US10570415B2

公开(公告)日：2020-02-25

申请号：US16054513

申请日：2018-08-03

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/113 ,  C07K19/00 ,  C12N15/52 ,  C12N15/62 ,  C12N15/88 ,  C12N9/10 ,  C12N15/74

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US20230295616A1

公开(公告)日：2023-09-21

申请号：US18127853

申请日：2023-03-29

Applicant: National Technology & Engineering Solutions of Sandia, LLC ,  The Regents of the University of California

Inventor： Oscar Negrete ,  Jennifer A. Doudna ,  Steven C. Strutt

IPC: C12N15/11 ,  C12N9/22 ,  C12Q1/6888 ,  A61K31/7088 ,  A61K38/46

CPC classification number: C12N15/11 ,  C12N9/22 ,  C12Q1/6888 ,  A61K31/7088 ,  A61K38/465 ,  C12N2800/80 ,  C12N2310/20

Abstract: The present invention relates to a CRISPR-Cas based system for targeting nucleic acid sequences. In part, the invention relates to synthetic guiding components for targeting single-stranded sequences, as well as design principles for constructing such components. Also described herein are methods of employing such components, e.g., to repress or activate a desired target within the subject.

公开(公告)号：US11661599B1

公开(公告)日：2023-05-30

申请号：US16219779

申请日：2018-12-13

Applicant: National Technology & Engineering Solutions of Sandia, LLC ,  The Regents of the University of California

Inventor： Oscar Negrete ,  Jennifer A. Doudna ,  Steven C. Strutt

IPC: C12N15/11 ,  C12N9/22 ,  C12Q1/6888 ,  A61K31/7088 ,  A61K38/46

CPC classification number: C12N15/11 ,  A61K31/7088 ,  A61K38/465 ,  C12N9/22 ,  C12Q1/6888 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present invention relates to a CRISPR-Cas based system for targeting nucleic acid sequences. In part, the invention relates to synthetic guiding components for targeting single-stranded sequences, as well as design principles for constructing such components. Also described herein are methods of employing such components, e.g., to repress or activate a desired target within the subject.

公开(公告)号：US11873504B2

公开(公告)日：2024-01-16

申请号：US16335516

申请日：2017-09-28

Applicant: The Regents of the University of California

Inventor： Jennifer A Doudna ,  Jillian F. Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  C12N15/86 ,  C12N15/90 ,  C07K19/00 ,  C12N9/10 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/88

CPC classification number: C12N15/8509 ,  C07K19/00 ,  C12N9/1007 ,  C12N9/1025 ,  C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/85 ,  C12N15/86 ,  C12N15/88 ,  C12N15/902 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/06 ,  C07K2319/09 ,  C07K2319/20 ,  C12N2310/20 ,  C12N2740/13043 ,  C12N2740/16043 ,  C12N2750/14143

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US20200017879A1

公开(公告)日：2020-01-16

申请号：US16335516

申请日：2017-09-28

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian F. Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/52 ,  C12N15/113 ,  C12N15/74 ,  C12N15/86 ,  C12N15/88 ,  C12N15/90 ,  C12N9/10 ,  C12N9/22

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US20180346927A1

公开(公告)日：2018-12-06

申请号：US16054513

申请日：2018-08-03

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/88 ,  C12N15/113 ,  C12N15/74 ,  C12N15/62 ,  C12N15/52 ,  C12N9/10 ,  C07K19/00

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.