公开(公告)号：US11521709B2

公开(公告)日：2022-12-06

申请号：US17325196

申请日：2021-05-19

申请人： BERKELEY LIGHTS, INC.

发明人： Darshan Thaker ,  Keith J. Breinlinger ,  Vincent Haw Tien Pai ,  Christoph Andreas Neyer ,  Thomas M. Vetterli ,  Hayley M. Bennett ,  Elisabeth Marie Walczak ,  Alexander Gerald Olson ,  Wesley Arthur Zink ,  John A. Tenney ,  Oleksandr Tokmakov ,  Igor Fastnacht ,  Yuriy Nicheporuk ,  Andriy Koval ,  Khrystyna Andres ,  Alona Kostenko

IPC分类号： G01N33/48 ,  G01N33/50 ,  G16B40/20 ,  G16B45/00 ,  G16B30/20 ,  B01L3/00

摘要： Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

公开(公告)号：US20230323433A1

公开(公告)日：2023-10-12

申请号：US18178927

申请日：2023-03-06

申请人： Berkeley Lights, Inc.

发明人： Matthew Asuka Kubit ,  Joshua David Mast ,  John Junyeon Kim ,  Alexander Gerald Olson ,  Preston Lock Ng ,  Arlvin Louis Ellefson ,  Shruthi Sreedhar Kubatur ,  Vincent Haw Tien Pai ,  Minha Park ,  Po-Yuan Tung ,  Jason C. Briggs ,  Patrick N. Ingram ,  Katrine Elise Dailey ,  Maryam Shansab ,  Jason M. McEwen ,  Adrienne T. Higa ,  Hongye Zhou ,  Zhen Hu ,  John A. Tenney

IPC分类号： C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6804

CPC分类号： C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6844 ,  C12Q2600/16

摘要： Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes.

公开(公告)号：US20230282313A1

公开(公告)日：2023-09-07

申请号：US17971400

申请日：2022-10-21

申请人： BERKELEY LIGHTS, INC.

发明人： Darshan Thaker ,  Keith J. Breinlinger ,  Vincent Haw Tien Pai ,  Christoph Andreas Neyer ,  Thomas M. Vetterli ,  Hayley M. Bennett ,  Elisabeth Marie Walczak ,  Alexander Gerald Olson ,  Wesley Arthur Zink ,  John A. Tenney ,  Oleksandr Tokmakov ,  Igor Fastnacht ,  Yuriy Nicheporuk ,  Andriy Koval ,  Khrystyna Andres ,  Alona Kostenko

IPC分类号： G16B40/20 ,  G16B45/00 ,  G16B30/20 ,  B01L3/00 ,  G01N15/14

CPC分类号： G16B40/20 ,  G16B45/00 ,  G16B30/20 ,  B01L3/502784 ,  G01N15/1463 ,  B01L2200/027

摘要： Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

公开(公告)号：US20210272654A1

公开(公告)日：2021-09-02

申请号：US17325196

申请日：2021-05-19

申请人： BERKELEY LIGHTS, INC.

发明人： Darshan Thaker ,  Keith J. Breinlinger ,  Vincent Haw Tien Pai ,  Christoph Andreas Neyer ,  Thomas M. Vetterli ,  Hayley M. Bennett ,  Elisabeth Marie Walczak ,  Alexander Gerald Olson ,  Wesley Arthur Zink ,  John A. Tenney ,  Oleksandr Tokmakov ,  Igor Fastnacht ,  Yuriy Nicheporuk ,  Andriy Koval ,  Khrystyna Andres ,  Alona Kostenko

IPC分类号： G16B40/20 ,  G16B30/20 ,  G16B45/00 ,  B01L3/00

摘要： Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).