公开(公告)号：US20140171380A1

公开(公告)日：2014-06-19

申请号：US14020776

申请日：2013-09-06

Applicant: The Trustees of Columbia University in the City of New York

Inventor： Tae-Wan Kim ,  Laura Beth Johnson McIntire ,  Natalie Landman ,  Gina Finan

IPC: G01N33/569 ,  A61K31/01 ,  A61K31/145 ,  A61K31/5375 ,  A61K31/15 ,  A61K31/7048 ,  A61K31/473 ,  A61K31/137 ,  A61K31/41

CPC classification number: G01N33/56966 ,  A61K31/01 ,  A61K31/03 ,  A61K31/085 ,  A61K31/137 ,  A61K31/145 ,  A61K31/15 ,  A61K31/41 ,  A61K31/473 ,  A61K31/5375 ,  A61K31/7048 ,  C12N5/0619 ,  C12N2501/385 ,  C12N2506/02 ,  C12N2533/50 ,  C12N2533/52 ,  G01N33/5058 ,  G01N2800/2821

Abstract: Successful CNS drug discovery requires a scalable, highly physiological neuronal model. Using directed differentiation of mouse embryonic stem (mES) cells, including mES cells isolated from a mouse model of Alzheimer's disease (AD), a highly homogeneous primary neuronal model amenable to phenotypic assays for production and synaptotoxicity of amyloid β-peptide was developed. This model furnishes a highly physiological and AD-relevant platform suitable for high throughput small molecule and functional genetic screens, providing specific small molecule compounds identified by such screens.

Abstract translation: 成功的CNS药物发现需要可扩展的，高度生理的神经元模型。 使用小鼠胚胎干（mES）细胞的定向分化，包括从阿尔茨海默氏病（AD）的小鼠模型分离的mES细胞，开发了适合淀粉样蛋白和生物肽的生产表型测定和突触毒性的高度均一的原代神经元模型。 该模型提供高生理和AD相关平台，适用于高通量小分子和功能遗传筛选，提供通过这种筛选鉴定的特异性小分子化合物。

公开(公告)号：US09696306B2

公开(公告)日：2017-07-04

申请号：US14020776

申请日：2013-09-06

Applicant: The Trustees of Columbia University in the City of New York

Inventor： Tae-Wan Kim ,  Laura Beth Johnson McIntire ,  Natalie Landman ,  Gina Finan

IPC: A61K31/137 ,  A61K31/03 ,  A61K31/085 ,  G01N33/569 ,  C12N5/0793 ,  G01N33/50 ,  A61K31/01 ,  A61K31/145 ,  A61K31/15 ,  A61K31/41 ,  A61K31/473 ,  A61K31/5375 ,  A61K31/7048

CPC classification number: G01N33/56966 ,  A61K31/01 ,  A61K31/03 ,  A61K31/085 ,  A61K31/137 ,  A61K31/145 ,  A61K31/15 ,  A61K31/41 ,  A61K31/473 ,  A61K31/5375 ,  A61K31/7048 ,  C12N5/0619 ,  C12N2501/385 ,  C12N2506/02 ,  C12N2533/50 ,  C12N2533/52 ,  G01N33/5058 ,  G01N2800/2821

Abstract: Successful CNS drug discovery requires a scalable, highly physiological neuronal model. Using directed differentiation of mouse embryonic stem (mES) cells, including mES cells isolated from a mouse model of Alzheimer's disease (AD), a highly homogeneous primary neuronal model amenable to phenotypic assays for production and synaptotoxicity of amyloid β-peptide was developed. This model furnishes a highly physiological and AD-relevant platform suitable for high throughput small molecule and functional genetic screens, providing specific small molecule compounds identified by such screens.